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公开(公告)号：US11439956B2
公开(公告)日：2022-09-13
申请号：US16864234
申请日：2020-05-01
发明人： Charles Heise , Tibor Nagy
摘要： Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means for mixing at least two liquids comprising a multiple inlet flow-controller, the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device configured in single-pass mode.
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公开(公告)号：US11306323B2
公开(公告)日：2022-04-19
申请号：US15838868
申请日：2017-12-12
发明人： Fay Louise Saunders , Anna Louise Dodds , Adeline Marie Geraldine Bayard , Bhupendra Vallabh Kara
摘要： A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.
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公开(公告)号：US09873891B2
公开(公告)日：2018-01-23
申请号：US14787669
申请日：2014-04-29
发明人： Fay Louise Saunders , Anna Louise Dodds , Adeline Marie Geraldine Bayard , Bhupendra Vallabh Kara
CPC分类号： C12N15/85 , C07K16/065 , C07K16/3092 , C07K2317/14 , C07K2319/02
摘要： A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.
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公开(公告)号：US09845475B2
公开(公告)日：2017-12-19
申请号：US14880671
申请日：2015-10-12
CPC分类号： C12N15/70 , C12N15/625
摘要： An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.
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公开(公告)号：US11685765B2
公开(公告)日：2023-06-27
申请号：US17152874
申请日：2021-01-20
发明人： Charles Heise , Tibor Nagy
IPC分类号： C07K1/34
CPC分类号： C07K1/34
摘要： A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.
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公开(公告)号：US20220275024A1
公开(公告)日：2022-09-01
申请号：US17634672
申请日：2020-08-06
发明人： Tibor Nagy , Jonathan Haigh , Charles Heise , Andrew Topping , James Pullen
IPC分类号： C07K1/36 , C07K1/34 , C07K1/22 , C07K1/18 , C07K16/06 , B01D15/14 , B01D15/18 , B01D61/14 , B01D15/38 , B01D15/36
摘要： A process for purifying a liquid feedstock comprising a monoclonal antibody and impurities, the process comprising passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified monoclonal antibody and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v) which include a multiple inlet flow-controller comprising two or more variable flow inlet valves for in situ production of a bioprocessing liquid by combining at least two liquids in a desired ratio. One of the units performs chromatography and another performs viral inactivation. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.
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公开(公告)号：US20210033574A1
公开(公告)日：2021-02-04
申请号：US16969690
申请日：2019-02-11
发明人： Charles Heise , Jonathan Haigh , Tibor Nagy , James Pullen , Andrew Topping
摘要： Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided. The apparatus comprises at least a first and a second means for carrying out a unit operation, each means for carrying out a unit operation comprising a feed for a liquid in fluid connection with the inlet of a flow-controller comprising a variable flow inlet valve and an outlet, wherein at least one of the means for carrying out a unit operation comprises feeds for at least two liquids, the feeds being in fluid connection with the inlets of a multiple inlet flow-controller comprising two or more variable flow inlet valves for dosing the at least two liquids, the flow-controller also comprising an outlet; a feed for a liquid feedstock comprising the target substance in fluid connection with the outlet from the flow-controller thereby to enable combination of the feed for a liquid comprising the target substance with the mixed bioprocessing liquids to produce a device feed; a device for achieving a processing operation comprising a device inlet and a device outlet, the device inlet being in fluid connection with the device feed; and a means for imparting flow through the flow controller and from the feed for the liquid feedstock through the processing device via the device inlet; and wherein the feed for a liquid feedstock comprising the target substance for the second means for carrying out a unit operation comprises the outlet from the first means for carrying out a unit operation.
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公开(公告)号：US20190010189A1
公开(公告)日：2019-01-10
申请号：US16068387
申请日：2016-12-19
发明人： Charles Heise , Tibor Nagy
IPC分类号： C07K1/34
摘要： A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.
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公开(公告)号：US20180127777A1
公开(公告)日：2018-05-10
申请号：US15838868
申请日：2017-12-12
发明人： Fay Louise Saunders , Anna Louise Dodds , Adeline Marie Geraldine Bayard , Bhupendra Vallabh Kara
CPC分类号： C12N15/85 , C07K16/065 , C07K16/3092 , C07K2317/14 , C07K2319/02
摘要： A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.
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公开(公告)号：US09677103B2
公开(公告)日：2017-06-13
申请号：US13966600
申请日：2013-08-14
IPC分类号： C12P21/00 , C12N15/70 , C12N15/72 , C12N15/78 , C12N15/81 , C12N15/85 , C12N15/73 , C12R1/19
CPC分类号： C12P21/00 , C12N15/70 , C12N15/72 , C12N15/73 , C12N15/78 , C12N15/81 , C12N15/85 , C12R1/19
摘要： A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.
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