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公开(公告)号：US20190227051A1
公开(公告)日：2019-07-25
申请号：US16219464
申请日：2018-12-13
IPC分类号： G01N33/487 , G01N15/12
摘要： A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A first salt buffer solution with a first osmolarity is flowed over a cell in the nanopore based sequencing chip to substantially fill a well in the cell with the first salt buffer solution. A lipid and solvent mixture is flowed over the cell to deposit a lipid membrane over the well that encloses the first salt buffer solution in the well. A second salt buffer solution with a second osmolarity is flowed above the well to reduce the thickness of the lipid membrane, wherein the second osmolarity is a lower osmolarity than the first osmolarity such that an osmotic imbalance is created between a first volume inside the well and a second volume outside the well.
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公开(公告)号：US10330633B2
公开(公告)日：2019-06-25
申请号：US15380721
申请日：2016-12-15
发明人： Roger J. A. Chen
IPC分类号： G01N27/327 , G01N33/487 , C12Q1/6874 , G01N27/447
摘要： A system for communicating information from an array of sensors is disclosed. The system comprises a sensor array that includes a plurality of sensors, wherein each sensor senses a physical property of a material that is in communication with the sensor. The system further comprises signal processing circuitry associated with each sensor that integrates the output of the sensor over time and compares the integrated output to a threshold. The system further comprises a communication network coupled to the signal processing circuitry that outputs information indicating that the integrated output corresponding to a given sensor has reached the threshold.
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公开(公告)号：US10260093B2
公开(公告)日：2019-04-16
申请号：US14355203
申请日：2012-11-01
发明人： Timothy Geiser , Randall Davis , Roger Chen
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6874 , C12Q1/6869 , B82Y15/00
摘要： Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.
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公开(公告)号：US20190085386A1
公开(公告)日：2019-03-21
申请号：US15809725
申请日：2017-11-10
发明人： Randall DAVIS , Roger CHEN
IPC分类号： C12Q1/6869 , G01N33/487 , G01N33/543 , C12N15/11
摘要： The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.
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公开(公告)号：US10234446B2
公开(公告)日：2019-03-19
申请号：US15699542
申请日：2017-09-08
发明人： Robert A. Yuan
IPC分类号： G01N27/447 , G01N33/487 , C12Q1/6869
摘要： A nanopore based sequencing system is disclosed. The system includes a plurality of nanopore sensors, each nanopore sensor having a top portion for receiving a fluid. The system further includes an inlet delivering the fluid into the nanopore based sequencing system and an outlet delivering the fluid out of the nanopore based sequencing system. The system includes a fluid chamber that comprises one or more fluid flow channels above top portions of the nanopore sensors; wherein the fluid chamber includes at least one divider that limits the width of the one or more fluid flow channels. In some embodiments, the at least one divider limits the width of the one or more fluid flow channel based on whether the surface tension and adhesive forces between the fluid and the fluid flow channel surfaces are sufficient to prevent the fluid from collapsing within the fluid flow channel.
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公开(公告)号：US20190004028A1
公开(公告)日：2019-01-03
申请号：US16021664
申请日：2018-06-28
发明人： Jennifer Hovis , Hui Tian , Roger J.A. Chen
IPC分类号： G01N33/487
CPC分类号： G01N33/48721
摘要： A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.
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7.发明申请公开(公告)号：US20190002968A1
公开(公告)日：2019-01-03
申请号：US15770922
申请日：2016-08-26
发明人： Frank BERGMANN , Christoph SEIDEL , Andrew TRANS , Dmitriy GREMYACHINSKIY , Hannes KUCHELMEISTER , Lars HILLRINGHAUS
IPC分类号： C12Q1/6869 , C07H19/10 , C07H19/20
摘要： The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.
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公开(公告)号：US20190002509A1
公开(公告)日：2019-01-03
申请号：US16134179
申请日：2018-09-18
摘要： The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein α-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.
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公开(公告)号：US10156541B2
公开(公告)日：2018-12-18
申请号：US15819994
申请日：2017-11-21
发明人： Roger J. A. Chen
IPC分类号： G01N27/327 , G01N33/487 , C12Q1/6874 , G01N27/447
摘要： A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.
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公开(公告)号：US20180328910A1
公开(公告)日：2018-11-15
申请号：US16046931
申请日：2018-07-26
IPC分类号： G01N33/487 , C12Q1/6869 , B82Y15/00
摘要： A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.
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