公开(公告)号：US08530222B2

公开(公告)日：2013-09-10

申请号：US12515602

申请日：2007-03-27

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12N1/20

CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

公开(公告)号：US07691619B2

公开(公告)日：2010-04-06

申请号：US11660959

申请日：2005-08-23

申请人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/02 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12P19/24 ,  C12N9/90

摘要： The invention relates to an isolated protein including an amino acid sequence represented by SEQ ID NO:2 and having an L-rhamnose isomerase activity. This novel enzyme has a higher reaction efficiency between D-psicose and D-allose and is excellent in thermal stability.

摘要翻译： 本发明涉及包含SEQ ID NO：2所示的氨基酸序列并具有L-鼠李糖异构酶活性的分离蛋白质。 该新型酶具有较高的D-木糖和D-丙氨酸的反应效率，热稳定性优异。

公开(公告)号：US20090325231A1

公开(公告)日：2009-12-31

申请号：US12515596

申请日：2007-03-26

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P21/00 ,  C12N9/90 ,  C12N1/21 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定的L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

公开(公告)号：US08227232B2

公开(公告)日：2012-07-24

申请号：US12515596

申请日：2007-03-26

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12N9/90 ,  C12N1/00 ,  C12P19/24 ,  C07H1/00 ,  C07H1/06

CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定性L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

公开(公告)号：US08748589B2

公开(公告)日：2014-06-10

申请号：US11817774

申请日：2006-03-03

申请人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

发明人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

IPC分类号： C07H1/00

CPC分类号： C12P19/02 ,  C12P19/24

摘要： A complex crystalline sugar comprising D-psicose and D-allose and a method for producing the same are disclosed. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.

摘要翻译： 公开了一种复合结晶糖，其包含D-木糖和D-阿洛糖及其制备方法。 糖中D-灵糖和D-阿洛糖的组成比为约1:1至1:4。 一种制备复合结晶糖的方法，该复合结晶糖包括D-木糖和D-阿洛糖，该方法包括由含有D-木糖和对 - 阿洛糖的糖溶液制备包含D-灵敏蛋白和D-阿洛糖的复合结晶糖并收集复合结晶糖 糖。 用于生产复合结晶糖的糖溶液的溶剂是水或水和乙醇的混合物。 通过包括使D-灵芝糖与L-鼠李糖异构酶反应以将D-灵芝蛋白转化为D-丙氨酸的方法产生含有D-灵敏蛋白和D-阿洛糖的糖溶液。 L-鼠李糖异构酶衍生自属于假单胞菌（Strepomonas stutzeri）的菌株（IPOD FERM BP-08593）。

公开(公告)号：US20090068710A1

公开(公告)日：2009-03-12

申请号：US11817774

申请日：2006-03-03

申请人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

发明人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

IPC分类号： C12P19/24 ,  C07H1/00

CPC分类号： C12P19/02 ,  C12P19/24

摘要： Disclosed is a process for producing a crystalline sugar comprising D-psicose and D-allose. Also disclosed is a process for producing the sugar.A complex crystalline sugar comprising D-psicose and D-allose. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.

摘要翻译： 公开了一种生产包含D-木糖和D-阿洛糖的结晶糖的方法。 还公开了生产糖的方法。 一种复合结晶糖，其包含D-木糖和D-丙氨酸。 糖中D-灵糖和D-阿洛糖的组成比为约1:1至1:4。 一种制备复合结晶糖的方法，该复合结晶糖包括D-木糖和D-阿洛糖，该方法包括由含有D-木糖和对 - 阿洛糖的糖溶液制备包含D-灵敏蛋白和D-阿洛糖的复合结晶糖并收集复合结晶糖 糖。 用于生产复合结晶糖的糖溶液的溶剂是水或水和乙醇的混合物。 通过包括使D-灵芝糖与L-鼠李糖异构酶反应以将D-灵芝蛋白转化为D-丙氨酸的方法产生含有D-灵敏蛋白和D-阿洛糖的糖溶液。 L-鼠李糖异构酶衍生自属于假单胞菌（Strepomonas stutzeri）的菌株（IPOD FERM BP-08593）。

公开(公告)号：US20090004694A1

公开(公告)日：2009-01-01

申请号：US11660959

申请日：2005-08-23

申请人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

IPC分类号： C12P19/24 ,  C07H21/04 ,  C12N9/90 ,  C12N15/63 ,  C12N1/00 ,  C12P21/00

CPC分类号： C12P19/24 ,  C12N9/90

摘要： [PROBLEMS] To provide the sequence of a thermotolerant L-rhamnose isomerase gene. [MEANS FOR SOLVING PROBLEMS] A DNA comprising the base sequence represented by SEQ ID NO:1. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A protein originating in Bacillus pallidus strain 14a (FERM AP-20172) and having an L-rhamnose isomerase activity. A protein having an L-rhamnose isomerase activity which is specified as having the following characteristics: optimum temperature and working temperature: showing the maximum enzymatic activity at 80° C. (the optimum temperature) and working temperature ranging from 30 to 80° C.; heat stability: concerning the effect of temperature on the enzymatic activity, being stable at up to 50° C. in the case of heating for 1 hour; and catalyzing the isomerization from D-psicose to D-allose. A method of producing D-allose which comprises treating a solution containing D-psicose with the above protein having the L-rhamnose isomerase activity as a catalyst at 35 to 80° C. to thereby convert D-psicose into D-allose.

摘要翻译： [问题]提供耐热L-鼠李糖异构酶基因的序列。 解决问题的方法包含SEQ ID NO：1所示的碱基序列的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种起源于苍白球菌菌株14a（FERM AP-20172）并具有L-鼠李糖异构酶活性的蛋白质。 具有L-鼠李糖异构酶活性的蛋白质被指定为具有以下特征：最佳温度和工作温度：在80℃（最佳温度）和工作温度范围为30至80℃时显示最大酶活性。 ; 热稳定性：关于温度对酶活性的影响，在加热1小时的情况下在高达50℃下是稳定的; 并催化从D-灵芝糖到D-丙氨酸的异构化。 一种D-丙氨酸的制造方法，其特征在于，在35〜80℃下，将上述具有L-鼠李糖异构酶活性的蛋白质作为催化剂处理含有D-灵芝糖的溶液，由此将D-木糖转化成D-阿洛糖。

公开(公告)号：US20100167350A1

公开(公告)日：2010-07-01

申请号：US12515602

申请日：2007-03-27

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P19/00 ,  C07H3/08 ,  C12N1/20

CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。