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公开(公告)号：US07964198B2
公开(公告)日：2011-06-21
申请号：US12380095
申请日：2009-02-24
IPC分类号： A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/70 , C12N7/00 , C12N2720/12351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2770/20051 , C12N2770/24051 , C12N2770/24151
摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.
摘要翻译： 用于生产哺乳动物病毒的方法和方法，所述方法包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下孵育感染病毒的培养物繁殖病毒，并形成含病毒的培养基， 并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 优点包括本发明的人细胞可以在规定的无血清条件下进行培养，并且细胞显示出增强的病毒传播能力。 提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还提供了连续或间歇地除去培养基。 因此，本发明允许病毒的大规模连续生产达到高滴度。
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公开(公告)号：US20090324645A1
公开(公告)日：2009-12-31
申请号：US12380095
申请日：2009-02-24
IPC分类号： A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/70 , C12N7/00 , C12N2720/12351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2770/20051 , C12N2770/24051 , C12N2770/24151
摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.
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公开(公告)号：US07521220B2
公开(公告)日：2009-04-21
申请号：US11256352
申请日：2005-10-21
IPC分类号： C12N7/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/70 , C12N7/00 , C12N2720/12351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2770/20051 , C12N2770/24051 , C12N2770/24151
摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.
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4.发明授权Means and methods for fibroblast-like or macrophage-like cell transduction 有权成纤维细胞样或巨噬细胞样细胞转导的方法和方法公开(公告)号：US06869936B1
公开(公告)日：2005-03-22
申请号：US09517898
申请日：2000-03-03
CPC分类号： C12N15/86 , A61K38/45 , A61K48/00 , C12N2710/10343 , C12N2710/10345 , C12N2810/6018
摘要： The invention provides a nucleic acid delivery vehicle with or having been provided with at least a tissue tropism for fibroblast-like or macrophage-like cells, preferably synoviocytes. In one aspect the nucleic acid delivery vehicle is a virus capsid or a functional part, derivative and/or analogue thereof. Preferably, the virus capsid is an adenovirus capsid. Preferably, the adenovirus is a subgroup B adenovirus, preferably adenovirus 16. Preferably, the tissue tropism is provided by at least a tissue tropism determining part of an adenovirus fiber protein or a functional derivative and/or analogue thereof. The invention further presents methods for the treatment of diseases, preferably joint related diseases.
摘要翻译： 本发明提供具有或已经提供至少一种成纤维细胞样细胞或巨噬细胞样细胞的组织嗜性的核酸递送载体，优选滑膜细胞。 一方面，核酸递送载体是病毒衣壳或其功能部分，衍生物和/或类似物。 优选地，病毒衣壳是腺病毒衣壳。 优选地，腺病毒是亚组B腺病毒，优选腺病毒16.优选地，组织向性由至少组织嗜性确定部分腺病毒纤维蛋白或其功能衍生物和/或类似物提供。 本发明还提供了用于治疗疾病，优选关节相关疾病的方法。
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