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    Production of vaccines
    2.
    发明授权
    Production of vaccines 有权
    生产疫苗

    公开(公告)号：US07964198B2

    公开(公告)日：2011-06-21

    申请号：US12380095

    申请日：2009-02-24

    申请人： Maria G. Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert J. Schouten

    发明人： Maria G. Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert J. Schouten

    IPC分类号： A61K39/145

    CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/70 ,  C12N7/00 ,  C12N2720/12351 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2770/20051 ,  C12N2770/24051 ,  C12N2770/24151

    摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.

    摘要翻译： 用于生产哺乳动物病毒的方法和方法，所述方法包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下孵育感染病毒的培养物繁殖病毒，并形成含病毒的培养基， 并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 优点包括本发明的人细胞可以在规定的无血清条件下进行培养，并且细胞显示出增强的病毒传播能力。 提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还提供了连续或间歇地除去培养基。 因此，本发明允许病毒的大规模连续生产达到高滴度。

    Production methods
    4.
    发明授权
    Production methods 有权
    生产方式

    公开(公告)号：US09163249B2

    公开(公告)日：2015-10-20

    申请号：US12674069

    申请日：2008-08-19

    申请人： Mark Uden ,  Ekaterini Kotsopoulou

    发明人： Mark Uden ,  Ekaterini Kotsopoulou

    IPC分类号： C12P21/06 ,  C12N15/67 ,  C07K16/00 ,  C07K16/18 ,  A61K39/12 ,  C12N7/02 ,  A61K39/00

    CPC分类号： C12N15/85 ,  A61K39/12 ,  A61K2039/525 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/41 ,  C07K2317/56 ,  C12N7/02 ,  C12N15/67 ,  C12N2770/24034 ,  C12N2770/24051 ,  C12N2800/60 ,  C12N2830/40

    摘要： The present invention provides methods of reducing the levels of a titratable selectable pressure required, the number of amplification cycles, and the time taken to generate protein expressing cell lines by altering the codons of the desired open-reading-frames. Through the use of codon adaptation for this purpose the methods of the invention consistently provide sufficient yields in faster time frames saving many weeks in cell line development activities. Furthermore the methods of the invention also generate cell lines with lower concentrations of selection and amplification agent than previously achievable. Accordingly lower levels of selection and amplification marker in the final cells lines are observed.

    摘要翻译： 本发明提供了通过改变期望的开放阅读框的密码子来降低所需的可滴定可选择压力水平，扩增循环次数以及产生蛋白质表达细胞系所花费的时间的方法。 通过为此目的使用密码子适配，本发明的方法在更快的时间内始终提供足够的产量，从而在细胞系发育活动中节省了许多个星期。 此外，本发明的方法还产生具有比先前可实现的更低浓度的选择和扩增剂的细胞系。 因此，观察到最终细胞系中选择和扩增标记的较低水平。

    Production of vaccines
    5.
    发明授权
    Production of vaccines 有权
    生产疫苗

    公开(公告)号：US07527961B2

    公开(公告)日：2009-05-05

    申请号：US11271368

    申请日：2005-11-11

    申请人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert Johan Schouten

    发明人： Maria Grazia Pau ,  Alphonsus G. C. M. UytdeHaag ,  Govert Johan Schouten

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5252 ,  A61K2039/70 ,  C12N2720/12351 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2770/20051 ,  C12N2770/24051 ,  C12N2770/24151

    摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.

    摘要翻译： 用于生产哺乳动物病毒的方法和方法，所述方法包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下孵育感染病毒的培养物繁殖病毒，并形成含病毒的培养基， 并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 优点包括本发明的人细胞可以在规定的无血清条件下进行培养，并且细胞显示出增强的病毒传播能力。 提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还提供了连续或间歇地除去培养基。 因此，本发明允许病毒的大规模连续生产达到高滴度。

    Method for in vitro culture of viruses of the togaviridae and flaviviridae families and uses
    8.
    发明申请
    Method for in vitro culture of viruses of the togaviridae and flaviviridae families and uses 审中-公开
    披膜病毒和黄病毒科的病毒体外培养方法及用途

    公开(公告)号：US20040229336A1

    公开(公告)日：2004-11-18

    申请号：US10776617

    申请日：2004-02-12

    申请人： Bio Merieux ,  Institut National De La Sante Et De La Recherche Medicale

    发明人： Patrice Andre ,  Vincent Lotteau ,  Glaucia Paranhos-Baccala ,  Florence Komurian-Pradel

    IPC分类号： C12N007/00

    CPC分类号： C12N7/00 ,  C12N2770/24051 ,  G01N33/56983 ,  G01N2333/18 ,  Y02A50/60

    摘要： The invention concerns a method of culturing, propagating and replicating in vitro viruses of the Togaviridae and Flaviviridae families which consists in: providing at least a fraction of LVP's obtained from serum or plasma of a patient infected with a least a virus of the Togaviridae and Flaviviridae families; contacting said fraction, for a predetermined time interval in an appropriate culture medium, with permissive cells having an endocytosis path relayed by at least one receptor of the lipoproteins and modulated inter alia by an activating agent selected among an unsaturated fatty acid or an unsaturated fatty acid derivative comprising 16 to 20 carbon atoms or their mixture. The invention also concerns the uses of viral particles and polypeptides obtained by said method.

    摘要翻译： 本发明涉及一种培养，繁殖和复制沙眼病毒科和黄病毒科的体外病毒的方法，该方法包括：提供至少一部分血清或血浆中获得的至少一种感染了披属病毒科和黄病毒科的病毒的患者的LVP 家庭; 在合适的培养基中使所述级分接触预定的时间间隔，其中容许细胞具有由至少一种脂蛋白受体中和的内吞途径，并特别通过选自不饱和脂肪酸或不饱和脂肪酸的活化剂调节 包含16-20个碳原子的衍生物或它们的混合物。 本发明还涉及通过所述方法获得的病毒颗粒和多肽的用途。