公开(公告)号：US20090017068A1

公开(公告)日：2009-01-15

申请号：US12220971

申请日：2008-07-29

申请人： Alphonsus G.C.M. UytdeHaag ,  Govert Johan Schouten ,  Jaap Goudsmit

发明人： Alphonsus G.C.M. UytdeHaag ,  Govert Johan Schouten ,  Jaap Goudsmit

IPC分类号： A61K39/12 ,  C12N7/04 ,  C12N5/08 ,  A61P31/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2770/24134 ,  C12N2770/24163

摘要： Described are vaccines containing (whole-inactivated) West Nile Viruses and/or West Nile viral proteins derived therefrom, produced on human cells, wherein the human cells comprise a sequence encoding at least one early region-1 (E1) gene product of an adenovirus. The cells are preferably cultured in suspension to very high densities and under serum-free conditions. Herein, it is disclosed that use of such cells results in high titers of West Nile Virus produced.

摘要翻译： 描述了在人细胞上产生的含有（全失活的）西尼罗病毒和/或由其衍生的西尼罗病毒蛋白质的疫苗，其中人细胞包含编码腺病毒的至少一个早期区域-1（E1）基因产物的序列 。 细胞优选在悬浮液中以非常高的密度和无血清条件培养。 在本文中，公开了使用这种细胞导致西尼罗病毒产生的高滴度。

公开(公告)号：US20090324645A1

公开(公告)日：2009-12-31

申请号：US12380095

申请日：2009-02-24

申请人： Maria G. Pau ,  Alphonsus G.C.M. UytdeHaag ,  Govert J. Schouten

发明人： Maria G. Pau ,  Alphonsus G.C.M. UytdeHaag ,  Govert J. Schouten

IPC分类号： A61K39/145

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/70 ,  C12N7/00 ,  C12N2720/12351 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2770/20051 ,  C12N2770/24051 ,  C12N2770/24151

摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.

公开(公告)号：US20090239287A1

公开(公告)日：2009-09-24

申请号：US12454095

申请日：2009-05-12

申请人： Maria Grazia Pau ,  Alphonsus G.C.M. UytdeHaag

发明人： Maria Grazia Pau ,  Alphonsus G.C.M. UytdeHaag

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  C12N2710/10343 ,  C12N2760/16134 ,  C12N2760/16152

摘要： Means and methods are provided for the production of mammalian viruses comprising: infecting a culture of immortalized human cells with the virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages are that human cells of the present invention can be cultured under defined serum free conditions, and the cells show improved capability for propagating virus. In particular, methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity to use whole chicken embryos for the production of influenza vaccines. The method provides also for the continuous or batchwise removal of culture media. As such, the invention allows the large-scale, continuous production of viruses to a high titer.

摘要翻译： 提供了用于生产哺乳动物病毒的方法和方法，包括：用病毒感染永生化人细胞的培养物，将感染病毒的培养物培养以在允许病毒生长的条件下繁殖病毒，并形成含病毒 培养基，并除去含病毒的培养基。 可以收获病毒并用于生产疫苗。 本发明的人细胞的优点在于能够在规定的无血清条件下进行培养，细胞表现出增强的病毒能力。 特别地，提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。 该方法消除了使用全鸡胚胎生产流感疫苗的必要性。 该方法还用于连续或间歇地除去培养基。 因此，本发明允许大规模，连续的病毒产生高滴度。