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    PLANT ARTIFICIAL CHROMOSOME COMPOSITIONS AND METHODS
    4.
    发明申请
    PLANT ARTIFICIAL CHROMOSOME COMPOSITIONS AND METHODS 失效
    植物人造革色素组合物和方法

    公开(公告)号：US20060003449A1

    公开(公告)日：2006-01-05

    申请号：US10161849

    申请日：2002-08-06

    Applicant: Daphne Preuss ,  Gregory Copenhaver

    Inventor： Daphne Preuss ,  Gregory Copenhaver

    IPC: A01H1/00 ,  C12N15/87 ,  C12N5/04

    CPC classification number: C12N15/82

    Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.

    Abstract translation: 本发明提供了拟南芥中功能性植物着丝粒的鉴定和克隆。 这将允许构建稳定遗传的植物人工染色体（PLAC），其可以用作构建转基因植物和动物细胞的载体。 此外，关于这些区域的结构和功能的信息将有助于分离来自其他物种的额外着丝粒和着丝粒相关的遗传元件和多肽。

    Plant artificial chromosome compositions and methods
    6.
    发明授权
    Plant artificial chromosome compositions and methods 失效
    植物人工染色体组成及方法

    公开(公告)号：US6156953A

    公开(公告)日：2000-12-05

    申请号：US90051

    申请日：1998-06-03

    Applicant: Daphne Preuss ,  Gregory Copenhaver

    Inventor： Daphne Preuss ,  Gregory Copenhaver

    IPC: C12N1/21 ,  C12N15/82 ,  C12N15/87 ,  A01H9/00 ,  A01H11/00 ,  A01H5/00 ,  A01H1/00

    CPC classification number: C07K14/415 ,  C12N15/82 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8258 ,  C12N15/8261

    Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.

    Abstract translation: 本发明提供了拟南芥中功能性植物着丝粒的鉴定和克隆。 这将允许构建稳定遗传的植物人工染色体（PLAC），其可以用作构建转基因植物和动物细胞的载体。 此外，关于这些区域的结构和功能的信息将有助于分离来自其他物种的额外着丝粒和着丝粒相关的遗传元件和多肽。

    Use of methylated nucleic acid segments for isolating centromere DNA
    8.
    发明授权
    Use of methylated nucleic acid segments for isolating centromere DNA 失效
    甲基化核酸片段用于分离着丝粒DNA的用途

    公开(公告)号：US06649347B2

    公开(公告)日：2003-11-18

    申请号：US09888220

    申请日：2001-06-22

    Applicant: Song Luo ,  Gregory Copenhaver ,  Kevin Keith ,  Daphne Preuss

    Inventor： Song Luo ,  Gregory Copenhaver ,  Kevin Keith ,  Daphne Preuss

    IPC: C12Q168

    CPC classification number: C12N15/1034 ,  C12N15/82 ,  C12Q1/6837 ,  C12Q2521/331

    Abstract: The invention provides efficient methods for the isolation of centromeres from potentially any organism. The methods may comprise the steps of: a) preparing a first sample of genomic DNA from a selected species; b) obtaining a plurality of methylated nucleic acid segments from the genomic DNA; and c) screening the methylated nucleic acid segments to identify a centromere nucleic acid sequence.

    Abstract translation: 本发明提供了将着丝粒从潜在的任何生物体中分离的有效方法。 所述方法可以包括以下步骤：a）从所选物种制备基因组DNA的第一样品; b）从基因组DNA获得多个甲基化核酸片段; 和c）筛选甲基化核酸片段以鉴定着丝粒核酸序列。

    Plants Modified With Mini-Chromosomes
    10.
    发明申请
    Plants Modified With Mini-Chromosomes 有权
    用小染色体修饰的植物

    公开(公告)号：US20080060093A1

    公开(公告)日：2008-03-06

    申请号：US10590546

    申请日：2005-02-23

    Applicant: Helge Zieler ,  James Jin ,  Jennifer Mach ,  Gary Rudgers ,  Daphne Preuss ,  Mich Hein ,  Gregory Copenhaver ,  Kevin Keith

    Inventor： Helge Zieler ,  James Jin ,  Jennifer Mach ,  Gary Rudgers ,  Daphne Preuss ,  Mich Hein ,  Gregory Copenhaver ,  Kevin Keith

    IPC: A01H1/00 ,  A01H5/00 ,  C07K14/00 ,  C12N15/64 ,  C12N9/00

    CPC classification number: C12N15/82

    Abstract: The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

    Abstract translation: 本发明通常涉及用新型自主小型染色体转化的植物的方法。 具有新组合物和结构的微型染色体用于转化依次用于产生植物的植物细胞。 用于产生植物的方法包括将微型染色体递送到植物细胞以转化细胞的方法，用于选择转化细胞的方法，以及分离用微型染色体转化的植物的方法。 本发明产生的植物含有通过整合入现有染色体而引入其基因组的新基因。

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