-
公开(公告)号：US5856123A
公开(公告)日：1999-01-05
申请号：US465986
申请日：1995-06-06
IPC分类号： C12N15/09 , C07H21/04 , C07K14/555 , C07K14/56 , C07K14/575 , C07K14/61 , C07K14/62 , C07K14/655 , C12N1/16 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C07K14/555 , C07K14/56 , C07K14/57581 , C07K14/61 , C07K14/62 , C07K14/655 , C12P21/02 , Y10S435/942
摘要： DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.
-
公开(公告)号：US5618676A
公开(公告)日：1997-04-08
申请号：US474333
申请日：1995-06-07
IPC分类号： C12N15/09 , C07H21/04 , C07K14/555 , C07K14/56 , C07K14/575 , C07K14/61 , C07K14/62 , C07K14/655 , C12N1/16 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/865 , C12N1/18 , C12N15/11 , C12P19/34 , C12P21/04
CPC分类号： C12N15/81 , C07K14/555 , C07K14/56 , C07K14/57581 , C07K14/61 , C07K14/62 , C07K14/655 , C12P21/02 , Y10S435/942
摘要： A DNA expression vector capable, in a transformant strain of yeast, of expressing a biologically competent polypeptide ordinarily exogenous to yeast under the control of a genetically distinct yeast promoter, the polypeptide not being required for the growth of the transformant; the process of forming the transformant strain of yeast; and the transformant strain of yeast.
-
公开(公告)号：US5919651A
公开(公告)日：1999-07-06
申请号：US465927
申请日：1995-06-06
IPC分类号： C12N15/09 , C07H21/04 , C07K14/555 , C07K14/56 , C07K14/575 , C07K14/61 , C07K14/62 , C07K14/655 , C12N1/16 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C07K14/555 , C07K14/56 , C07K14/57581 , C07K14/61 , C07K14/62 , C07K14/655 , C12P21/02 , Y10S435/942
摘要： DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.
摘要翻译： 公开了在转基因酵母菌株中能够在遗传上不同的酵母启动子控制下表达多肽的DNA表达载体，形成酵母和转化酵母菌株的转化菌株的方法。
-
公开(公告)号：US06475489B1
公开(公告)日：2002-11-05
申请号：US08482156
申请日：1995-06-07
IPC分类号： A61K3929
CPC分类号： C07K14/005 , A61K39/00 , C12N15/81 , C12N2730/10122 , Y10S530/806 , Y10S530/824 , Y10S530/826
摘要： The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBSAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAq particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
摘要翻译： 本发明涉及酵母中HBsAg的合成。 已经构建了包含酵母启动子ADH1的酵母表达载体。 编码S蛋白的HBV基因组区域（不包括可能的163个氨基酸的前序列）已被转移到酵母表达载体中。 使用描述的酵母载体，已经实现了通过酵母成功合成HBsAg。 该产物具有抗原性（与抗HBsAg反应），并且发现大部分与电子显微镜外观相同的颗粒与HBV感染患者和亚历山大细胞血清中发现的颗粒相关，但具有较小的粒径直径。 由酵母合成的HBSAg与通过蔗糖梯度沉降测量的从Alexander细胞纯化的天然存在的HBsAq颗粒相同的沉淀行为。 本发明证明了通过在微生物中表达异源DNA编码区而产生的更有序的多组分结构的合成和装配。
-
公开(公告)号：US5854018A
公开(公告)日：1998-12-29
申请号：US465931
申请日：1995-06-06
IPC分类号： C12N15/09 , C07H21/04 , C07K14/555 , C07K14/56 , C07K14/575 , C07K14/61 , C07K14/62 , C07K14/655 , C12N1/16 , C12N1/19 , C12N1/21 , C12N15/00 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C07K14/555 , C07K14/56 , C07K14/57581 , C07K14/61 , C07K14/62 , C07K14/655 , C12P21/02 , Y10S435/942
摘要： DNA expression vectors capable, in a transformant strain of yeast, of expressing a polypeptide under the control of a genetically distinct yeast promoter, processes of forming transformant strains of yeast and transformed yeast strains are disclosed.
-
公开(公告)号：US06544757B1
公开(公告)日：2003-04-08
申请号：US07209504
申请日：1988-06-21
IPC分类号： C12P2106
CPC分类号： C07K14/005 , A61K39/00 , C12N15/81 , C12N2730/10122 , Y10S530/806 , Y10S530/824 , Y10S530/826
摘要： The present invention relates to synthesis of HBSAg in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBSAg by yeast has been achieved. The product is antigenic (reactive with anti-HBSAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBSAg synthesized by yeast has identical sedimentation behavior to purified, naturally occurring HBSAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
摘要翻译： 本发明涉及酵母中HBSAg的合成。 已经构建了包含酵母启动子ADH1的酵母表达载体。 编码S蛋白的HBV基因组区域（不包括可能的163个氨基酸的前序列）已被转移到酵母表达载体中。 使用描述的酵母载体，已经实现了酵母成功合成HBSAg。 该产物具有抗原性（与抗HBSAg反应），并且发现大部分与电子显微镜外观相同的颗粒与HBV感染患者和亚历山大细胞血清中发现的颗粒相关，但具有较小的粒径直径。 由酵母合成的HBSAg与通过蔗糖梯度沉降测量的从亚历山大细胞纯化的天然存在的HBSAg颗粒具有相同的沉淀行为。 本发明证明了通过在微生物中表达异源DNA编码区而产生的更有序的多组分结构的合成和装配。
-
7.发明授权DNA-sequence encoding biosynthetic insulin precursors and process for preparing the insulin precursors and human insulin 失效编码生物合成胰岛素前体的DNA序列和制备胰岛素前体和人胰岛素的方法
公开(公告)号：US4916212A
公开(公告)日：1990-04-10
申请号：US739123
申请日：1985-05-29
申请人： Jan Markussen , Niels Fiil , Mogens T. Hansen , Kjeld Norris , Gustav Ammerer , Lars Thim , Hans O. Voigt
发明人： Jan Markussen , Niels Fiil , Mogens T. Hansen , Kjeld Norris , Gustav Ammerer , Lars Thim , Hans O. Voigt
IPC分类号： C07K14/575 , C07K14/62 , C12N1/16 , C12N1/19 , C12N15/00 , C12N15/09 , C12N15/17 , C12N15/62 , C12N15/69 , C12N15/81 , C12P21/02 , C12R1/865
CPC分类号： C12N15/69 , C07K14/62 , C12N15/62 , C12N15/81 , C07K2319/02 , Y10S435/942
摘要： Human insulin precursors containing the peptide chain B(1-29)-A(1-21) of human insulin and derivatives thereof with a bridging chain connecting the carboxyl terminus of the B(1-29)-chain with the amino terminus of the A(1-21)-chain are prepared by culturing a yeast host transformed with a replicable expression vehicle capable of expressing a DNA-sequence encoding the insulin precursor. The bridging chain is preferably relatively short and contains preferably from 2 to 8 amino acid residues. The bridging chain must not contain two adjacent basic amino acid residues (Lys or Arg) and has one Lys or Arg connected to the amino terminus of the A(1-21)-chain. Human insulin is prepared from the insulin precursors by in vitro conversion.
摘要翻译： 含有人胰岛素的肽链B（1-29）-A（1-21）及其衍生物的人胰岛素前体与将B（1-29）链的羧基末端与氨基末端连接的桥连链 通过培养用能够表达编码胰岛素前体的DNA序列的可复制表达载体转化的酵母宿主制备A（1-21）链。 桥连链优选相对较短，优选含有2-8个氨基酸残基。 桥连链不能包含两个相邻的碱性氨基酸残基（Lys或Arg），并且具有连接到A（1-21）链氨基末端的一个Lys或Arg。 通过体外转化从胰岛素前体制备人胰岛素。
-
公开(公告)号：US4769238A
公开(公告)日：1988-09-06
申请号：US821146
申请日：1985-12-12
CPC分类号： C07K14/005 , C12N15/81 , A61K39/00 , C12N2730/10122 , Y10S530/806 , Y10S530/824 , Y10S530/826
摘要： The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADHl, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector.Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBsAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
摘要翻译： 本发明涉及酵母中HBsAg的合成。 已经构建了包含酵母启动子ADH1的酵母表达载体。 编码S蛋白的HBV基因组区域（不包括可能的163个氨基酸的前序列）已被转移到酵母表达载体中。 使用描述的酵母载体，已经实现了通过酵母成功合成HBsAg。 该产物具有抗原性（与抗HBsAg反应），并且发现大部分与电子显微镜外观相同的颗粒与HBV感染患者和亚历山大细胞血清中发现的颗粒相关，但具有较小的粒径直径。 由酵母合成的HBsAg与通过蔗糖梯度沉降测量的从亚历山大细胞纯化的天然存在的HBsAg颗粒具有相同的沉淀行为。 本发明证明了通过在微生物中表达异源DNA编码区而产生的更有序的多组分结构的合成和装配。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.011 秒)
