-
公开(公告)号：US08598143B2
公开(公告)日：2013-12-03
申请号：US12273673
申请日：2008-11-19
IPC分类号： A01N43/04
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/11 , C12N2310/113 , C12N2310/321 , C12N2320/50 , C12Q1/6897 , C12N2310/3521
摘要： The present invention relates to the discovery of a method for inhibiting RNA silencing in a target sequence-specific manner. RNA silencing requires a set of conserved cellular factors to suppress expression of gene-encoded polypeptide. The invention provides compositions for sequence-specific inactivation of the RISC component of the RNA silencing pathway, and methods of use thereof. The RISC inactivators of the present invention enable a variety of methods for identifying and characterizing miRNAs and siRNAs, RISC-associated factors, and agents capable of modulating RNA silencing. Therapeutic methods and compositions incorporating RISC inactivators and therapeutic agents identified through use of RISC inactivators are also featured.
摘要翻译： 本发明涉及以目标序列特异性方式发现抑制RNA沉默的方法。 RNA沉默需要一组保守的细胞因子来抑制基因编码的多肽的表达。 本发明提供了用于RNA沉默途径的RISC组分的序列特异性失活的组合物及其使用方法。 本发明的RISC灭活剂能够用于鉴定和表征miRNA和siRNA，RISC相关因子和能调节RNA沉默的多种方法。 还列出了通过使用RISC灭活剂鉴定的包含RISC灭活剂和治疗剂的治疗方法和组合物。
-
公开(公告)号：US20100184827A1
公开(公告)日：2010-07-22
申请号：US12748689
申请日：2010-03-29
IPC分类号： A61K31/7052 , C07H21/02 , C12N15/63 , C12N5/071 , A61P43/00
CPC分类号： C12N15/11 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/113 , C12N2310/111 , C12N2310/14 , C12N2310/331 , C12N2310/333 , C12N2310/336 , C12N2320/51
摘要： The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
-
3.发明授权公开(公告)号：US07691995B2
公开(公告)日：2010-04-06
申请号：US10195034
申请日：2002-07-12
IPC分类号： C07H21/04
CPC分类号： C12N15/113 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N15/111 , C12N15/8509 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/30 , C12N2330/30 , C12N2330/51 , C12N2517/02 , C12N2799/021 , C12N2830/008
摘要： The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.
摘要翻译： 本发明提供了工程化的RNA前体，其在细胞中表达时被细胞加工以产生靶向小干扰RNA（siRNA），其使用细胞自身的RNA干扰（RNAi）途径选择性沉默靶基因（通过切割特异性mRNA）。 通过将具有合适的调节序列将这些工程化RNA前体编码的核酸分子引入细胞体内，工程化RNA前体的表达可以在时间和空间上选择性地控制，即在特定时间和/或特别是组织，器官或 细胞。
-
4.发明授权Methods and compositions for enhancing the efficacy and specificity of RNA silencing 有权用于增强RNA沉默的功效和特异性的方法和组合物公开(公告)号：US08304530B2
公开(公告)日：2012-11-06
申请号：US12729892
申请日：2010-03-23
CPC分类号： C12N15/11 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/113 , C12N2310/111 , C12N2310/14 , C12N2310/331 , C12N2310/333 , C12N2310/336 , C12N2320/51
摘要： The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
摘要翻译： 本发明提供增强RNA沉默的功效和特异性的方法。 本发明还提供介导RNA沉默的组合物。 特别地，本发明提供了siRNA，siRNA样分子，shRNA，载体和转基因，其具有改善的靶基因沉默的特异性和功效。 还介绍了治疗方法。
-
公开(公告)号：US20100184826A1
公开(公告)日：2010-07-22
申请号：US12729892
申请日：2010-03-23
IPC分类号： A61K31/713 , C07H21/02 , A61P31/00 , A61P35/00 , A61P37/00
CPC分类号： C12N15/11 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/113 , C12N2310/111 , C12N2310/14 , C12N2310/331 , C12N2310/333 , C12N2310/336 , C12N2320/51
摘要： The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
-
公开(公告)号：US20100173407A1
公开(公告)日：2010-07-08
申请号：US12526612
申请日：2008-01-30
申请人： Krzysztof Wypijewski , Christophe Lacomme , Gyorgy Hutvagner , Csaba Hornyik , Jane Shaw , Jennifer Stephens
发明人： Krzysztof Wypijewski , Christophe Lacomme , Gyorgy Hutvagner , Csaba Hornyik , Jane Shaw , Jennifer Stephens
CPC分类号： C12N15/63
摘要： There is provided a method of inhibiting gene expression by locating a 5′-5 donor splicing site sequence in the 3′ UTR of a gene or within coding sequence containing the stop codon of a gene. Stability of homologous mRNA in trans is also adversely affected leading to a reduction in expression. A polynucleotide containing a 5′-donor splicing site sequence in either coding sequence containing the stop codon or the 3′ UTR is also provided and in one embodiment is in the form of a vector. The 5′-donor splicing site sequence can be present in multiple copies, for example as a tandem repeat. In one embodiment the 5-donor splicing site sequence has the sequence 5′-MAGGTRAGTA-3′ where M is A or C and R is A or G.
摘要翻译： 提供了通过将5'-5供体剪接位点序列定位在基因的3'UTR中或在含有基因终止密码子的编码序列内来抑制基因表达的方法。 同源mRNA在反式中的稳定性也受到不利影响，导致表达降低。 还提供了含有包含终止密码子或3'UTR的编码序列中的5'-供体剪接位点序列的多核苷酸，在一个实施方案中为载体形式。 5'供体剪接位点序列可以以多个拷贝存在，例如作为串联重复。 在一个实施方案中，5-供体剪接位点序列具有序列5'-MAGGTRAGTA-3'，其中M为A或C，R为A或G.
-
7.发明申请公开(公告)号：US20060009402A1
公开(公告)日：2006-01-12
申请号：US10195034
申请日：2002-07-12
IPC分类号： A61K48/00
CPC分类号： C12N15/113 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N15/111 , C12N15/8509 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/30 , C12N2330/30 , C12N2330/51 , C12N2517/02 , C12N2799/021 , C12N2830/008
摘要： The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.
摘要翻译： 本发明提供了工程化的RNA前体，其在细胞中表达时被细胞加工以产生靶向小干扰RNA（siRNA），其使用细胞自身的RNA干扰（RNAi）途径选择性沉默靶基因（通过切割特异性mRNA）。 通过将具有合适的调节序列将这些工程化RNA前体编码的核酸分子引入细胞体内，工程化RNA前体的表达可以在时间和空间上选择性地控制，即在特定时间和/或特别是组织，器官或 细胞。
-
8.发明授权公开(公告)号：US08557785B2
公开(公告)日：2013-10-15
申请号：US12729360
申请日：2010-03-23
IPC分类号： C12N15/11
CPC分类号： C12N15/113 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N15/111 , C12N15/8509 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/30 , C12N2330/30 , C12N2330/51 , C12N2517/02 , C12N2799/021 , C12N2830/008
摘要： The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.
摘要翻译： 本发明提供了工程化的RNA前体，其在细胞中表达时被细胞加工以产生靶向小干扰RNA（siRNA），其使用细胞自身的RNA干扰（RNAi）途径选择性沉默靶基因（通过切割特异性mRNA）。 通过将具有合适的调节序列将这些工程化RNA前体编码的核酸分子引入细胞体内，工程化RNA前体的表达可以在时间和空间上选择性地控制，即在特定时间和/或特别是组织，器官或 细胞。
-
公开(公告)号：US08309705B2
公开(公告)日：2012-11-13
申请号：US12748937
申请日：2010-03-29
CPC分类号： C12N15/11 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/113 , C12N2310/111 , C12N2310/14 , C12N2310/331 , C12N2310/333 , C12N2310/336 , C12N2320/51
摘要： The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured.
-
10.发明申请公开(公告)号：US20110207224A1
公开(公告)日：2011-08-25
申请号：US13031522
申请日：2011-02-21
CPC分类号： C12N15/113 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N15/111 , C12N15/8509 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2320/30 , C12N2330/30 , C12N2330/51 , C12N2517/02 , C12N2799/021 , C12N2830/008
摘要： The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vitro with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.
摘要翻译： 本发明提供了工程化的RNA前体，其在细胞中表达时被细胞加工以产生靶向小干扰RNA（siRNA），其使用细胞自身的RNA干扰（RNAi）途径选择性沉默靶基因（通过切割特异性mRNA）。 通过将具有合适调控序列的这些工程化RNA前体编码的核酸分子引入体外，工程改造的RNA前体的表达可以在时间上和空间上选择性地控制，即在特定时间和/或特别是组织，器官或 细胞。
-
-
-
-
-
-
-
-
-
搜索结果
22 条记录 (耗时 0.031 秒)
