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公开(公告)号:US09181530B2
公开(公告)日:2015-11-10
申请号:US13978839
申请日:2012-01-10
申请人: Suzanne U. Emerson , Priyanka Shukla , Hanh T. Nguyen , Robert H. Purcell , Harry R. Dalton , Richard Bendall
发明人: Suzanne U. Emerson , Priyanka Shukla , Hanh T. Nguyen , Robert H. Purcell , Harry R. Dalton , Richard Bendall
IPC分类号: A61K39/29 , A61K39/12 , C07H21/00 , C12N7/00 , C12N15/86 , C12Q1/70 , C07K14/005 , A61K39/00
CPC分类号: C12N7/00 , A61K39/00 , C07K14/005 , C07K14/47 , C12N15/86 , C12N2770/28121 , C12N2770/28122 , C12N2770/28134 , C12N2770/28143 , C12N2770/28151 , C12N2770/28152 , C12Q1/707
摘要: The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.
摘要翻译: 本发明涉及从长期感染的患者中发现HEV毒株。 病毒在许多细胞培养物中异常生长。 因此,本发明提供了基于病毒的细胞培养物,载体和疫苗组合物。 本发明部分地涉及鉴定新型HEV基因型3病毒株。 使用从慢性感染患者中分离的HEV菌株Kernow-C1(基因型3),用于鉴定允许感染的人,猪和鹿细胞系。 将Kernow-C 1菌株适应于人肝癌细胞中生长,其选择含有人核糖体蛋白基因的174个核糖核苷酸(58个氨基酸)和另外的突变的稀有病毒重组体。
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公开(公告)号:US20130302790A1
公开(公告)日:2013-11-14
申请号:US13978839
申请日:2012-01-10
申请人: Suzanne U. Emerson , Priyanka Shukla , Hanh T. Nguyen , Robert H. Purcell , Harry R. Dalton , Richard Bendall
发明人: Suzanne U. Emerson , Priyanka Shukla , Hanh T. Nguyen , Robert H. Purcell , Harry R. Dalton , Richard Bendall
CPC分类号: C12N7/00 , A61K39/00 , C07K14/005 , C07K14/47 , C12N15/86 , C12N2770/28121 , C12N2770/28122 , C12N2770/28134 , C12N2770/28143 , C12N2770/28151 , C12N2770/28152 , C12Q1/707
摘要: The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations.
摘要翻译: 本发明涉及从长期感染的患者中发现HEV毒株。 病毒在许多细胞培养物中异常生长。 因此,本发明提供了基于病毒的细胞培养物,载体和疫苗组合物。 本发明部分地涉及鉴定新型HEV基因型3病毒株。 使用从慢性感染患者中分离的HEV菌株Kernow-C1(基因型3),用于鉴定允许感染的人,猪和鹿细胞系。 将Kernow-C 1菌株适应于人肝癌细胞中生长,其选择含有人核糖体蛋白基因的174个核糖核苷酸(58个氨基酸)和另外的突变的稀有病毒重组体。
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