公开(公告)号：US5981701A

公开(公告)日：1999-11-09

申请号：US474691

申请日：1995-06-07

Applicant: David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

IPC: C12N15/09 ,  A61K38/00 ,  A61P9/00 ,  A61P37/06 ,  A61P37/08 ,  C07H21/04 ,  C07K1/16 ,  C07K1/20 ,  C07K14/00 ,  C07K14/47 ,  C07K14/52 ,  C07K14/525 ,  C07K14/715 ,  C07K16/00 ,  C07K16/18 ,  C07K16/24 ,  C07K16/28 ,  C07K19/00 ,  C12P21/00 ,  C12P21/08 ,  C12R1/19 ,  C07K14/705

CPC classification number: C07K14/7151 ,  C07K16/2866 ,  A61K38/00

Abstract: Tumor Necrosis Factor (TNF) Inhibitory Protein is isolated and substantially purified. It has the ability to inhibit: (a) the binding of TNF to its receptors, and (b) the cytotoxic effect of TNF. TNF Inhibitory Protein and salts, functional derivatives and active fractions thereof can be used to antagonize the deleterious effects of TNF.

Abstract translation: 肿瘤坏死因子（TNF）抑制蛋白被分离并基本纯化。 它具有抑制：（a）TNF与其受体的结合，和（b）TNF的细胞毒性作用。 TNF抑制蛋白及其盐，功能衍生物及其活性成分可用于拮抗TNF的有害作用。

公开(公告)号：US5811261A

公开(公告)日：1998-09-22

申请号：US126016

申请日：1993-09-24

Applicant: David Wallach ,  Yaron Nophar ,  Oliver Kemper ,  Hartmut Engelmann ,  Cord Brakebusch ,  Dan Aderka

Inventor： David Wallach ,  Yaron Nophar ,  Oliver Kemper ,  Hartmut Engelmann ,  Cord Brakebusch ,  Dan Aderka

IPC: A61K38/00 ,  C07K14/715 ,  C07K16/28 ,  C12N15/85 ,  C12P21/06

CPC classification number: C07K14/7151 ,  C07K16/2866 ,  C12N15/85 ,  A61K38/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/55

Abstract: Tumor Necrosis Factor Binding Protein I (TBP-I), precursors and analogs thereof, are produced by transfecting eukaryotic cells with an expression vector comprising al DNA molecule encoding the whole human type I TNF receptor or a soluble domain thereof, and culturing the transfected cells, whereby the soluble proteins are secreted into the medium.

Abstract translation: 通过用包含编码整个人I型TNF受体的al DNA分子或其可溶性结构域的表达载体转染真核细胞产生肿瘤坏死因子结合蛋白I（TBP-1），其前体及其类似物，并培养转染的细胞 ，由此将可溶性蛋白质分泌到培养基中。

公开(公告)号：US5621077A

公开(公告)日：1997-04-15

申请号：US329785

申请日：1994-10-27

Applicant: Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

Inventor： Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

IPC: G01N33/53 ,  A61K38/00 ,  A61K38/21 ,  A61K39/395 ,  A61P35/00 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/555 ,  C07K14/565 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C12N15/12 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC classification number: C07K14/715 ,  A61K38/215 ,  C07K14/7155 ,  Y10S530/834

Abstract: Human natural IFN-.beta.2/IL-6 receptor and its soluble extracellular fragment are provided in substantially purified form and are shown to stimulate and to enhance beneficial effects of IFN-.beta.2/IL-6, such as its antiproliferative activity. Polyclonal and monoclonal antibodies raised against the soluble fragment of the receptor are also provided.

Abstract translation: 以天然IFN-β2/ IL-6受体及其可溶性细胞外片段提供基本上纯化的形式，并显示刺激和增强IFN-β2/ IL-6的有益效果，如其抗增殖活性。 还提供针对受体的可溶性片段产生的多克隆和单克隆抗体。

公开(公告)号：US5359037A

公开(公告)日：1994-10-25

申请号：US563151

申请日：1990-08-06

Applicant: David Wallach ,  Hartmut Engelmann ,  Dan Aderka

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka

IPC: A61K38/00 ,  C07K14/715 ,  C07K16/28 ,  C07K15/00

CPC classification number: C07K16/2866 ,  C07K14/7151 ,  A61K38/00

Abstract: Antibodies to TNF Binding Protein I (TBP-I) are provided that can be used in diagnostic assays and as pharmaceutical agents for either inhibiting or mimicking the effects of TNF on cells.

Abstract translation: 提供了TNF结合蛋白I（TBP-1）的抗体，其可用于诊断测定和用作抑制或模拟TNF对细胞的作用的药物。

公开(公告)号：US08138323B2

公开(公告)日：2012-03-20

申请号：US10319536

申请日：2002-12-16

Applicant: David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Daniela Novick ,  Menachem Rubinstein

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Daniela Novick ,  Menachem Rubinstein

IPC: C12N15/28 ,  C12N15/79 ,  C12N15/74 ,  C12N5/10 ,  C12N1/21

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151

Abstract: An isolated DNA molecule has the sequence of encoding a fragment of encoding Tumor Necrosis Factor (TNF) Binding Protein II. It has a length sufficient to serve as an immunogen for raising antibodies against a polypeptide that is a fragment of TNF Binding Protein II. The DNA may be used to produce replicable expression vehicles and prokaryotic or eukaryotic expression host cells. Such host cells may be used to produce polypeptides encoded by such DNA molecules.

Abstract translation: 分离的DNA分子具有编码肿瘤坏死因子（TNF）结合蛋白II的片段的序列。 其长度足以用作用于产生针对作为TNF结合蛋白II的片段的多肽的抗体的免疫原。 DNA可用于产生可复制的表达载体和原核或真核表达宿主细胞。 这样的宿主细胞可用于产生由这样的DNA分子编码的多肽。

公开(公告)号：US06262239B1

公开(公告)日：2001-07-17

申请号：US08476862

申请日：1995-06-07

Applicant: David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

Inventor： David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

IPC: C07K1628

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151 ,  C07K16/4258 ,  C07K2319/00

Abstract: Antibodies to Tumor Necrosis Factor receptors (TNF-Rs) which inhibit the cytocidal effect of TNF but not its binding to the TNF-Rs, and ligands interacting with other receptors of the TNF/NGF family, are provided together with methods of producing them. The antibodies preferably bind to the fourth cysteine rich domain of the p75 TNF receptor or to the region between said fourth cysteine rich domain and the cell membrane.

公开(公告)号：US5695953A

公开(公告)日：1997-12-09

申请号：US876828

申请日：1992-04-30

Applicant: David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

IPC: C12N15/09 ,  A61K38/00 ,  A61P9/00 ,  A61P37/06 ,  A61P37/08 ,  C07H21/04 ,  C07K1/16 ,  C07K1/20 ,  C07K14/00 ,  C07K14/47 ,  C07K14/52 ,  C07K14/525 ,  C07K14/715 ,  C07K16/00 ,  C07K16/18 ,  C07K16/24 ,  C07K16/28 ,  C07K19/00 ,  C12P21/00 ,  C12P21/08 ,  C12R1/19 ,  C12P21/06 ,  C07H21/00

CPC classification number: C07K14/7151 ,  C07K16/2866 ,  A61K38/00

Abstract: Tumor Necrosis Factor (TNF) Inhibitory Protein is isolated and substantially purified and the DNA that encodes the TNF inhibitory protein, vectors, host cells, and a recombinant method for producing the encoded protein are also set forth. It has the ability to inhibit: (a) the binding of TNF to its receptors, and (b) the cytotoxic effect of TNF. TNF Inhibitory Protein and salts, functional derivatives and active fractions thereof can be used to antagonize the deleterious effects of TNF.

Abstract translation: 分离并基本纯化肿瘤坏死因子（TNF）抑制蛋白，并且还阐述了编码TNF抑制蛋白的DNA，载体，宿主细胞和用于产生编码蛋白质的重组方法。 它具有抑制：（a）TNF与其受体的结合，和（b）TNF的细胞毒性作用。 TNF抑制蛋白及其盐，功能衍生物及其活性成分可用于拮抗TNF的有害作用。

公开(公告)号：US07642046B2

公开(公告)日：2010-01-05

申请号：US10492520

申请日：2002-10-14

Applicant: Hartmut Engelmann

Inventor： Hartmut Engelmann

IPC: C12Q1/00 ,  G01N33/53 ,  G01N33/68

CPC classification number: G01N33/6872

Abstract: The present invention relates to an immunological method and kit for monitoring recruitment of a protein to the intracellular domain of a receptor in intact cells.

Abstract translation: 本发明涉及用于监测蛋白质在完整细胞中的受体的细胞内结构域的募集的免疫学方法和试剂盒。

公开(公告)号：US20050245731A1

公开(公告)日：2005-11-03

申请号：US11085057

申请日：2005-03-22

Applicant: David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Menachem Rubinstein

IPC: C12N15/09 ,  A61K38/00 ,  A61P9/00 ,  A61P37/06 ,  A61P37/08 ,  C07H21/04 ,  C07K1/16 ,  C07K1/20 ,  C07K14/00 ,  C07K14/47 ,  C07K14/52 ,  C07K14/525 ,  C07K14/715 ,  C07K16/00 ,  C07K16/18 ,  C07K16/24 ,  C07K16/28 ,  C07K19/00 ,  C12P21/00 ,  C12P21/08 ,  C12R1/19

CPC classification number: C07K14/7151 ,  A61K38/00 ,  C07K16/2866

Abstract: Recombinant DNA encoding Tumor Necrosis Factor (TNF) Inhibitory Protein, or an active fragment thereof, is obtained. The TNF Inhibitory Protein has the ability to inhibit: (a) the binding of TNF to its receptors, and (b) the cytotoxic effect of TNF. TNF Inhibitory Protein and salts, functional derivatives and active fractions thereof can be used to antagonize the deleterious effects of TNF by eliminating TNF from the body, and to reduce the cytotoxic activity of TNF by binding to TNF and thereby to inhibit the binding of TNF to its receptors. The DNA may be in an expression vector. Host cells transfected with such an expression vector may be used to produce the TNF Inhibitory Protein.

Abstract translation: 获得编码肿瘤坏死因子（TNF）抑制蛋白或其活性片段的重组DNA。 TNF抑制蛋白具有抑制（a）TNF与其受体的结合的能力，和（b）TNF的细胞毒性作用。 TNF抑制蛋白及其盐，功能衍生物及其活性成分可用于通过从体内消除TNF来拮抗TNF的有害作用，并通过与TNF结合而降低TNF的细胞毒活性，从而抑制TNF与TNF结合 其受体。 DNA可以是表达载体。 用这种表达载体转染的宿主细胞可用于产生TNF抑制蛋白。

公开(公告)号：US5216128A

公开(公告)日：1993-06-01

申请号：US530580

申请日：1990-05-30

Applicant: Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

Inventor： Daniela Novick ,  Louise Chen ,  Hartmut Engelmann ,  Michel Revel ,  Menachem Rubinstein ,  David Wallach

IPC: G01N33/53 ,  A61K38/00 ,  A61K38/21 ,  A61K39/395 ,  A61P35/00 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/555 ,  C07K14/565 ,  C07K14/705 ,  C07K14/715 ,  C07K16/00 ,  C12N15/12 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC classification number: C07K14/715 ,  A61K38/215 ,  C07K14/7155 ,  Y10S530/834

Abstract: Human natural IFN-.beta.2/IL-6 receptor and its soluble extracellular fragment are provided in substantially purified form and are shown to stimulate and to enhance beneficial effects of IFN-.beta.2/IL-6, such as its antiproliferative activity. Polyclonal and monoclonal antibodies raised against the soluble fragment of the receptor are also provided.