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1.发明申请公开(公告)号：US20120122130A1
公开(公告)日：2012-05-17
申请号：US11886885
申请日：2006-03-27
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％以下麦芽糖的活性; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集连接到FAD以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：US20090176262A1
公开(公告)日：2009-07-09
申请号：US12396724
申请日：2009-03-03
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12Q1/54
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。
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3.发明授权公开(公告)号：US08691547B2
公开(公告)日：2014-04-08
申请号：US11886885
申请日：2006-03-27
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％以下麦芽糖的活性; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集连接到FAD以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：US07514250B2
公开(公告)日：2009-04-07
申请号：US10540025
申请日：2003-12-24
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12N9/02
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。
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公开(公告)号：US20190040440A1
公开(公告)日：2019-02-07
申请号：US16145168
申请日：2018-09-28
申请人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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6.发明授权Method of determining 1,5-anhydroglucitol, and reagent composition for determining 1,5-anhydroglucitol 有权1,5-脱水葡萄糖醇的测定方法和1,5-脱水葡萄糖醇的试剂组合物公开(公告)号：US08945864B2
公开(公告)日：2015-02-03
申请号：US12305941
申请日：2007-06-22
申请人： Emi Ishimaru , Hirokazu Sanada , Hironori Omura , Hideki Yoshioka , Shuhei Tsukamoto , Minoru Masuda
发明人： Emi Ishimaru , Hirokazu Sanada , Hironori Omura , Hideki Yoshioka , Shuhei Tsukamoto , Minoru Masuda
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/66 , G01N33/6893 , G01N2800/042
摘要： The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.
摘要翻译： 本发明的目的是提供一种测定1,5-脱水葡萄糖醇的方法，包括使用（a）由SEQ ID NO：2的氨基酸序列组成的蛋白质; （b）由在SEQ ID NO：2的氨基酸序列中具有缺失，取代和/或添加一个或多个氨基酸残基并具有山梨糖脱氢酶活性的氨基酸序列组成的蛋白质; 或（c）由与SEQ ID NO：2的氨基酸序列具有至少60％的同源性并具有山梨糖脱氢酶活性的氨基酸序列组成的蛋白质。
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公开(公告)号：US20100297743A1
公开(公告)日：2010-11-25
申请号：US12851668
申请日：2010-08-06
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
IPC分类号： C12M1/34
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。
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公开(公告)号：US20090255656A1
公开(公告)日：2009-10-15
申请号：US12225434
申请日：2007-03-30
CPC分类号： F28F9/02 , B23K1/0012 , B23K1/16 , B23K2101/14 , F28D1/0391 , F28D2021/007 , F28F9/0243 , Y10T29/49378 , Y10T29/49389
摘要： A method of manufacturing a brazed pipe comprises steps A to D. In the step A, on an upper surface of the right-hand side edge portion of a material plate 20 having a brazing material layer over opposite surfaces thereof, a first slant surface 21 is formed such that it inclines from the upper side toward the lower side while approaching the right end, and a first flat surface 22 is formed between the first slant surface 21 and the lower surface. In the step B, on a lower surface of the left-hand side edge portion of the material plate 20, a second slant surface 24 is formed such that it inclines from the lower side toward the upper side while approaching the left end, and a second flat surface 25 is formed between the second slant surface 24 and the lower surface. In the step C, the material plate 20 is formed into a tubular shape such that the slant surfaces 21 and 24 are in surface contact with each other, and the flat surfaces 22 and 25 abut against each other, whereby a brazed-pipe tubular body 34 is obtained. In the step D, the slant surfaces 21 and 24 and the flat surfaces 22 and 25 of the opposite side edge portions of the material plate 20 are respectively brazed together by making use of the brazing material layer of the material plate 20. According to the method of the present invention, a brazed pipe which has no step on the outer surface can be provided.
摘要翻译： 制造钎焊管的方法包括步骤A至D.在步骤A中，在其相对表面上具有钎料层的材料板20的右侧边缘部分的上表面上具有第一倾斜表面21 形成为使其从靠近右端侧的上侧向下侧倾斜，在第一倾斜面21与下表面之间形成有第一平坦面22。 在步骤B中，在材料板20的左侧边缘部分的下表面上形成第二倾斜表面24，使得其在靠近左端时从下侧向上侧倾斜，并且 第二平坦表面25形成在第二倾斜表面24和下表面之间。 在步骤C中，材料板20形成为使得倾斜表面21和24彼此表面接触的管状形状，并且平坦表面22和25彼此抵接，由此钎焊管状体 34。 在步骤D中，通过利用材料板20的钎料层将材料板20的相对侧边缘部分的倾斜表面21和24以及平坦表面22和25分别钎焊在一起。根据 可以提供本发明的方法，在外表面上没有台阶的钎焊管。
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公开(公告)号：US20060063217A1
公开(公告)日：2006-03-23
申请号：US10540025
申请日：2003-12-24
申请人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Tetsunari Morita , Mika Kuyama , Tokuji Ikeda , Kenji Kano , Seiya Tsujimura
CPC分类号： C12N9/0004 , C12Q1/32 , C12Q1/54
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
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