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    Novel Vector
    1.
    发明申请
    Novel Vector 审中-公开
    小说矢量

    公开(公告)号：US20070277264A1

    公开(公告)日：2007-11-29

    申请号：US10559072

    申请日：2004-06-03

    申请人： Kazuya Nanto ,  Keiko Watanabe ,  Hiroyasu Ebinuma

    发明人： Kazuya Nanto ,  Keiko Watanabe ,  Hiroyasu Ebinuma

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/8209 ,  C12N15/821

    摘要： In order to provide a vector and a method for introducing one copy of a desired gene to a predetermined position of a host DNA at a high frequency without any bad influences upon the host cell and a vector and a method for deleting or inverting a host DNA at a predetermined position without a crossing step and without any bad influences upon the host cell. A vector which has an introduction cassette inserted between two site-specific recombinase recognition sequences and a site-specific recombinase gene which recognizes these site-specific recombinase recognition sequences, wherein the recombinase gene is positioned outside the introduction cassette, is used, and this is introduced into a host cell having a DNA in which one or two site-specific recombinase recognition sequences are present. A recombinant of interest can be efficiently obtained by positioning a lethal induction gene or a morphological abnormality induction gene outside the introduction cassette, together with the site-specific recombinase gene.

    摘要翻译： 为了提供一种将所需基因的一个拷贝以高频率引入宿主DNA的预定位置的载体和方法，对宿主细胞和载体没有任何不良影响，以及用于删除或翻译宿主DNA的方法 在没有交叉步骤的预定位置处，并且对宿主细胞没有任何不良影响。 使用具有插入两个位点特异性重组酶识别序列之间的引物盒和识别这些位点特异性重组酶识别序列的位点特异性重组酶基因的载体，其中重组酶基因位于引入盒外部，这是 引入具有其中存在一个或两个位点特异性重组酶识别序列的DNA的宿主细胞。 通过将导入盒外的致死诱导基因或形态异常诱导基因与位点特异性重组酶基因一起定位，可以有效地获得感兴趣的重组体。

    Method for efficiently producing transgenic plant using auxin precursor
    2.
    发明授权
    Method for efficiently producing transgenic plant using auxin precursor 失效
    使用生长素前体有效生产转基因植物的方法

    公开(公告)号：US07294761B2

    公开(公告)日：2007-11-13

    申请号：US10626609

    申请日：2003-07-25

    申请人： Etsuko Matsunaga ,  Koichi Sugita ,  Hiroyasu Ebinuma

    发明人： Etsuko Matsunaga ,  Koichi Sugita ,  Hiroyasu Ebinuma

    IPC分类号： C12N15/82 ,  C12N15/83 ,  C12N15/84 ,  C12N15/31 ,  C12N15/54 ,  C12N15/55

    CPC分类号： C12N15/821

    摘要： A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.

    摘要翻译： 一种生产转基因植物的方法，其包括：（A）将载体导入植物细胞，其中所述载体是用于将基因导入植物的载体，并且包含：所需基因和选择标记基因，所述选择标记基因包含编码 从生长素前体合成生长素的酶; （B）在生长素前体和/或其类似物的存在下培养载体引入基因的植物细胞，由此制备再分化组织，并检测并选择再分化组织; （C）培养在（B）中选择的再分化组织以重新分化植物个体和将基因导入植物的载体，其包含：所需基因和包含吲哚乙酰胺水解酶，iaaH基因和 异戊烯基转移酶，ipt，基因，并且不含色氨酸单加氧酶，iaaM基因。

    DNA encoding a transcription factor controlling phenylpropanoid biosynthesis pathway
    4.
    发明授权
    DNA encoding a transcription factor controlling phenylpropanoid biosynthesis pathway 有权
    编码控制苯丙素生物合成途径的转录因子的DNA

    公开(公告)号：US06303847B1

    公开(公告)日：2001-10-16

    申请号：US09282146

    申请日：1999-03-31

    申请人： Akiyoshi Kawaoka ,  Hiroyasu Ebinuma

    发明人： Akiyoshi Kawaoka ,  Hiroyasu Ebinuma

    IPC分类号： A01H500

    CPC分类号： C07K14/415 ,  C12N15/8243

    摘要： The present invention relates to an isolated and purified DNA having a nucleotide sequence which comprises SEQ ID NO:1, and encodes a transcription factor controlling a phenylpropanoid biosynthesis pathway; a recombinant vector comprising the DNA; the recombinant vector, further comprising a promoter to which the DNA is operably fused; the recombinant vector, wherein the DNA is operably fused to the promoter in the sense or antisense direction; a plant cell into which the DNA has been introduced; a plant regenerated from the plant cell; and an isolated and purified DNA which encodes a protein having the amino acid sequence of SEQ ID NO:2.

    摘要翻译： 本发明涉及具有包含SEQ ID NO：1的核苷酸序列的分离和纯化的DNA，并编码控制苯丙素类生物合成途径的转录因子; 包含该DNA的重组载体; 所述重组载体还包含可操作地融合所述DNA的启动子; 重组载体，其中DNA在正义或反义方向上可操作地与启动子融合; 已经引入DNA的植物细胞; 从植物细胞再生的植物; 以及编码具有SEQ ID NO：2的氨基酸序列的蛋白质的分离和纯化的DNA。

    METHOD FOR PRODUCTION OF PLANT CELL HAVING CHROMOSOME LOSS
    5.
    发明申请
    METHOD FOR PRODUCTION OF PLANT CELL HAVING CHROMOSOME LOSS 审中-公开
    用于生产染色体损失的植物细胞的方法

    公开(公告)号：US20090320164A1

    公开(公告)日：2009-12-24

    申请号：US11721273

    申请日：2005-12-08

    申请人： Kazuya Nanto ,  Hiroyasu Ebinuma

    发明人： Kazuya Nanto ,  Hiroyasu Ebinuma

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  C12N15/82

    摘要： The present invention provides a simple aneuploid production process, applicable to all species, without producing unexpected damage to chromosomes other than a chromosome which is to be disappeared. A vector comprising two site-specific recognition sequences oriented in the opposite direction, or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence, and a recombinase gene is introduced into a plant cells or a vector comprising two site-specific recognition sequences oriented in the opposite directions or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence is introduced into a plant cell; a recombinase is allowed to act transiently in the cell during at least growth of the cell; and the cell is cultured and grown; and a cell in which a predetermined chromosome is disappeared is selected.

    摘要翻译： 本发明提供了一种适用于所有物种的简单的非整倍体生产方法，而不会对将要消失的染色体以外的染色体产生意外的损害。 将包含相反方向取向的两个位点特异性识别序列或包含点对称核苷酸序列的一个位点特异性重组酶识别序列和重组酶基因的载体导入植物细胞或包含两个位点特异性识别的载体 将指向相反方向的序列或包含点对称核苷酸序列的一个位点特异性重组酶识别序列引入植物细胞中; 允许重组酶在细胞的至少生长期间暂时在细胞中起作用; 并培养和培养细胞; 并且选择其中预定染色体消失的细胞。

    PROCESS FOR PRODUCING PLANT STORAGE ORGAN WITH HIGH PRODUCTION OF RECOMBINANT PROTEIN AND NOVEL RECOMBINANT PROTEIN
    6.
    发明申请
    PROCESS FOR PRODUCING PLANT STORAGE ORGAN WITH HIGH PRODUCTION OF RECOMBINANT PROTEIN AND NOVEL RECOMBINANT PROTEIN 审中-公开
    生产重组蛋白和新型重组蛋白的植物储存制剂的生产工艺

    公开(公告)号：US20110203016A1

    公开(公告)日：2011-08-18

    申请号：US12765711

    申请日：2010-04-22

    申请人： Koichi SUGITA ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Fumio Takaiwa

    发明人： Koichi SUGITA ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Fumio Takaiwa

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N15/63

    CPC分类号： C12N15/8257 ,  C07K14/605 ,  C12N15/8216

    摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.

    摘要翻译： 本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。 通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素 相关基因和耐药基因存在于可以与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置 元件。 通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

    Plant and Plant Storage Organ Having Glp-1 Derivative Accumulated Therein and Method of Producing the Same
    7.
    发明申请
    Plant and Plant Storage Organ Having Glp-1 Derivative Accumulated Therein and Method of Producing the Same 失效
    含有Glp-1衍生物的植物和植物储存器官及其生产方法

    公开(公告)号：US20080292732A1

    公开(公告)日：2008-11-27

    申请号：US11662650

    申请日：2004-09-14

    申请人： Koichi Sugita ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Fumio Takaiwa ,  Hiroshi Yasuda ,  Takahito Jomori ,  Yuji Hayashi ,  Akira Tashita

    发明人： Koichi Sugita ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Fumio Takaiwa ,  Hiroshi Yasuda ,  Takahito Jomori ,  Yuji Hayashi ,  Akira Tashita

    IPC分类号： A61K36/00 ,  C12N15/82 ,  A01H5/00 ,  A61P31/10

    CPC分类号： A61K38/26 ,  A23L33/105 ,  C12N15/8257

    摘要： The present invention is to provide a plant and a plant storage organ thereof in which GLP-1 derivatives are accumulated, and a method of producing them in order to develop a method for ingesting orally GLP-1 derivatives at a low cost and to make use of it in diabetic treatment. A transgenic plant or a plant storage organ thereof in which GLP-1 derivatives are accumulated cleavable with a digestive enzyme is produced by a method comprising: integrating into a vector a linked GLP-1s-DNA which comprises tandem repeated “n” DNAs (“n” being an integer of 3 or more) encoding a GLP-1 derivative consisting of GLP-1 (7-36) or of an amino acid sequence of GLP-1 (7-36) in which one or a few amino acids are deleted, substituted and/or added, and C-terminal consists of Arg or Lys, and having GLP-1 activity; introducing the vector into a plant cell; and redifferentiating the obtained transformant. The transgenic plant and the plant storage organ are useful as a pharmaceutical composition or a food or drink for treating or preventing diabetes.

    摘要翻译： 本发明提供一种其中积聚有GLP-1衍生物的植物和植物贮藏器官及其生产方法，以便开发低成本摄取口服GLP-1衍生物的方法并使用 的糖尿病治疗。 GLP-1衍生物可以用消化酶积聚可切割的转基因植物或植物贮藏器官是通过以下方法产生的：将连接的GLP-1s-DNA整合到载体中，所述连接的GLP-1s-DNA包含串联重复的“n”DNA（“ 编号由GLP-1（7-36）或GLP-1（7-36）的氨基酸序列组成的GLP-1衍生物的n“为3以上的整数，其中一个或几个氨基酸为 缺失，取代和/或添加，C-末端由Arg或Lys组成，具有GLP-1活性; 将载体导入植物细胞; 并对所得转化体进行重新分化。 转基因植物和植物贮藏器官可用作药物组合物或用于治疗或预防糖尿病的食物或饮料。

    Vector for gene transfer into plant allowing optional deletion of marker gene
    8.
    发明授权
    Vector for gene transfer into plant allowing optional deletion of marker gene 失效
    用于基因转移到植物中的载体，允许标记基因的任选缺失

    公开(公告)号：US06326192B1

    公开(公告)日：2001-12-04

    申请号：US09147241

    申请日：1998-11-09

    申请人： Koichi Sugita ,  Mikiko Uesugi ,  Etsuko Matsunaga ,  Hiroyasu Ebinuma

    发明人： Koichi Sugita ,  Mikiko Uesugi ,  Etsuko Matsunaga ,  Hiroyasu Ebinuma

    IPC分类号： C12N1582

    CPC分类号： C12N15/8205 ,  C12N15/8201 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8242

    摘要： The present invention relates to a vector for introducing a desired gene into a plant, wherein a selectable marker gene introduced into a plant cell along with a desired gene is optionally removable from the DNA such as chromosome or the like where it exists and functions, then disappeared the function thereof after its expression, and the expression of the selectable marker gene and the disappearance of the function thereof are detectable by morphological change of the tissue derived from the plant cell into which the selectable marker gene is introduced. Also, the present invention constitutes a vector using a morphological abnormality induction gene as a selectable marker gene, while putting a removable DNA element under control of an inducible promoter, wherein the morphological abnormality induction gene is positioned such that it behaves integrally with the removable DNA element, and wherein a desired gene is positioned such that it does not behave integrally with the removable DNA element.

    摘要翻译： 本发明涉及用于将期望的基因导入植物中的载体，其中与所需基因一起导入植物细胞的选择性标记基因可任选地从其存在并起作用的染色体等DNA脱除，然后 其表达后的功能消失，并且可选择标记基因的表达及其功能的消失可以通过导入选择性标记基因的植物细胞衍生的组织的形态学变化来检测。 此外，本发明构成使用形态异常诱导基因作为选择性标记基因的载体，同时将可移除的DNA元件置于诱导型启动子的控制下，其中形态异常诱导基因定位成使其与可除去的DNA整体地起作用 元件，并且其中将期望的基因定位成使得其不与可移除的DNA元件整体表现。

    Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same
    10.
    发明授权
    Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same 失效
    蓄积有GLP-1衍生物的植物贮藏器具及其制造方法

    公开(公告)号：US07947876B2

    公开(公告)日：2011-05-24

    申请号：US11662650

    申请日：2004-09-14

    申请人： Koichi Sugita ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Humio Takaiwa ,  Hiroshi Yasuda ,  Takahito Jomori ,  Yuji Hayashi ,  Akira Tashita

    发明人： Koichi Sugita ,  Saori Kasahara ,  Hiroyasu Ebinuma ,  Humio Takaiwa ,  Hiroshi Yasuda ,  Takahito Jomori ,  Yuji Hayashi ,  Akira Tashita

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： A61K38/26 ,  A23L33/105 ,  C12N15/8257

    摘要： The present invention is regarding plants and plant storage organs thereof in which GLP-1 derivatives are accumulated, and methods of producing them. The transgenic plants and plant storage organs thereof accumulate tandem repeated GLP-1 derivatives cleavable with intestinal digestive enzyme to monomeric molecules and are produced by methods comprising: integrating into vectors linked DNAs which comprise tandem repeated DNAs encoding the GLP-1 derivative with trypsin resistance in which the amino acid in the 26th position is Gln, the amino acid in the 34th position is Asn or Asp, and C-terminal consists of Arg or Lys to produce monomeric molecules; introducing the vectors into plant cells; and redifferentiating the obtained transformants. The edible transgenic plants and plant storage organs are useful for treating diabetes and can be ingested by diabetic patients.

    摘要翻译： 本发明涉及其中积累GLP-1衍生物的植物和植物贮藏器官及其生产方法。 其转基因植物和植物储存器官将可与肠消化酶切割的串联重复的GLP-1衍生物积聚到单体分子中，并通过以下方法产生：包括：将包含编码GLP-1衍生物的串联重复DNA与胰蛋白酶抗性的连锁DNA连接 其中第26位的氨基酸是Gln，第34位的氨基酸是Asn或Asp，C末端由Arg或Lys组成以产生单体分子; 将载体导入植物细胞; 并重新分化获得的转化体。 可食用的转基因植物和植物贮藏器官可用于治疗糖尿病并可被糖尿病患者摄入。