公开(公告)号：US20050095640A1

公开(公告)日：2005-05-05

申请号：US11013179

申请日：2004-12-15

申请人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

发明人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q2545/101

摘要： Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

公开(公告)号：US20060115851A1

公开(公告)日：2006-06-01

申请号：US11339364

申请日：2006-01-25

申请人： Hrissi Samartzidou ,  Thomas Houts ,  Wen Yang ,  Son Bui ,  Timothy Harkins

发明人： Hrissi Samartzidou ,  Thomas Houts ,  Wen Yang ,  Son Bui ,  Timothy Harkins

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6888 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2545/101 ,  C12Q2545/113

摘要： Method of producing universal controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from intergenic or intronic regions of a genome. Twenty-three specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

公开(公告)号：US06943242B2

公开(公告)日：2005-09-13

申请号：US10140545

申请日：2002-05-07

申请人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

发明人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q2545/101

摘要： Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

摘要翻译： 生产用于基因表达分析系统如大阵列，实时PCR，Northern印迹，SAGE和微阵列的对照的方法。 对照是从DNA的近随机序列或基因组的内部或内部区域产生的。 还公开了10个特异性控制序列。 还提出了使用这些对照的方法，包括作为阴性对照，阳性对照和作为基因表达分析系统的校准物。