公开(公告)号：US20050095640A1

公开(公告)日：2005-05-05

申请号：US11013179

申请日：2004-12-15

申请人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

发明人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q2545/101

摘要： Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

公开(公告)号：US20060115851A1

公开(公告)日：2006-06-01

申请号：US11339364

申请日：2006-01-25

申请人： Hrissi Samartzidou ,  Thomas Houts ,  Wen Yang ,  Son Bui ,  Timothy Harkins

发明人： Hrissi Samartzidou ,  Thomas Houts ,  Wen Yang ,  Son Bui ,  Timothy Harkins

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6888 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2545/101 ,  C12Q2545/113

摘要： Method of producing universal controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from intergenic or intronic regions of a genome. Twenty-three specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

公开(公告)号：US06943242B2

公开(公告)日：2005-09-13

申请号：US10140545

申请日：2002-05-07

申请人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

发明人： Hrissi Samartzidou ,  Leah Turner ,  Steven Daniel ,  Thomas Houts

IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q2545/101

摘要： Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

摘要翻译： 生产用于基因表达分析系统如大阵列，实时PCR，Northern印迹，SAGE和微阵列的对照的方法。 对照是从DNA的近随机序列或基因组的内部或内部区域产生的。 还公开了10个特异性控制序列。 还提出了使用这些对照的方法，包括作为阴性对照，阳性对照和作为基因表达分析系统的校准物。

公开(公告)号：US20060234270A1

公开(公告)日：2006-10-19

申请号：US11379223

申请日：2006-04-18

申请人： Thomas Houts

发明人： Thomas Houts

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  G16B25/00 ,  C12Q2545/114 ,  C12Q2527/137

摘要： The invention provides methods for obtaining an expected concentration for each of a plurality of housekeeping genes and using these values to obtain normalized concentrations for genes in an experimental sample using both external transcripts and internal housekeeping genes. A standard calibration curve is prepared using intensity values and concentrations of external transcripts. The standard calibration curve is used to obtain an expected concentration for each housekeeping gene. A calibration curve in an experimental sample is used to obtain preliminary concentrations for housekeeping genes and a correction factor is calculated from the expected concentrations. The correction factor is used to normalize values for genes in the experimental sample.

摘要翻译： 本发明提供了获得多个管家基因中的每一个的预期浓度的方法，并且使用这些值使用外部转录物和内部管理基因来获得实验样品中的基因的归一化浓度。 使用强度值和外部转录物浓度制备标准校准曲线。 标准校正曲线用于获得每个管家基因的预期浓度。 使用实验样品中的校准曲线获得持家基因的初步浓度，并根据预期浓度计算校正因子。 校正因子用于标准化实验样品中基因的值。