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公开(公告)号：US5654495A
公开(公告)日：1997-08-05
申请号：US383756
申请日：1995-02-02
CPC分类号： A23D9/00 , C11B1/00 , C12N15/8247 , C12N9/16
摘要： By this invention, methods to produce C14 fatty acids in plant seed oils are provided. In a first embodiment, this invention relates to particular C14 preferring acyl-ACP thioesterase sequences from Cuphea palustris, camphor and nutmeg, and to DNA constructs for the expression of these thioesterases in host cells for production of C14 fatty acids. Other aspects of this invention relate to methods for using other plant medium-chain thioesterases or medium-chain thioesterases from non-plant sources to provide C14 fatty acids in plant cells. In this regard, the production of C14 fatty acids in plant cells as the result of expression from an elm medium chain acyl-ACP thioesterase and a bacterial luxD gene is provided.
摘要翻译： 通过本发明，提供了在植物种子油中生产C14脂肪酸的方法。 在第一个实施方案中，本发明涉及来自Cuphea palustris，樟脑和肉豆蔻的特定C14优选的酰基-ACP硫酯酶序列，以及用于在宿主细胞中表达这些硫酯酶以产生C14脂肪酸的DNA构建体。 本发明的其它方面涉及使用来自非植物来源的其它植物中链硫酯酶或中链硫酯酶在植物细胞中提供C14脂肪酸的方法。 在这方面，提供了作为从榆中链酰基-ACP硫酯酶和细菌luxD基因表达的结果在植物细胞中产生C14脂肪酸。
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公开(公告)号：US6093568A
公开(公告)日：2000-07-25
申请号：US231196
申请日：1994-04-21
申请人： Huw Maelor Davies , Deborah Hawkins , Janet Nelsen
发明人： Huw Maelor Davies , Deborah Hawkins , Janet Nelsen
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants are considered.
摘要翻译： 本发明涉及植物LPAAT，鉴定此类蛋白质的方法，与此类蛋白质相关的氨基酸和核酸序列，以及获得，制备和/或使用此类植物LPAAT的方法。 净化，特别是植物膜的去除以及远离其它植物蛋白质的大量分离，并且提供了植物LPAAT的使用，包括使用蛋白质作为用于生物技术应用的基因分离的工具。 此外，提供了编码LPAAT蛋白质区域的核酸序列，并且考虑了这些序列用于从植物中分离LPAAT基因的用途。
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公开(公告)号：US5910630A
公开(公告)日：1999-06-08
申请号：US327451
申请日：1994-10-21
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are considered.
摘要翻译： 本发明涉及植物LPAAT，鉴定此类蛋白质的方法，与此类蛋白质相关的氨基酸和核酸序列，以及获得，制备和/或使用此类植物LPAAT的方法。 净化，特别是植物膜的去除以及远离其它植物蛋白质的大量分离，并且提供了植物LPAAT的使用，包括使用蛋白质作为用于生物技术应用的基因分离的工具。 另外，提供了编码LPAAT蛋白质区域的核酸序列，并且考虑了这样的序列用于从植物中分离LPAAT基因和修饰植物甘油三酯组合物的用途。
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公开(公告)号：US5824858A
公开(公告)日：1998-10-20
申请号：US254404
申请日：1994-06-06
申请人： Huw Maelor Davies , Deborah Hawkins , Janet Nelsen
发明人： Huw Maelor Davies , Deborah Hawkins , Janet Nelsen
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are considered.
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公开(公告)号：US5639790A
公开(公告)日：1997-06-17
申请号：US142473
申请日：1993-11-18
IPC分类号： A01H5/00 , A01H5/10 , A23D9/00 , C07K14/415 , C11B1/00 , C11C3/00 , C12N5/10 , C12N9/16 , C12N15/09 , C12N15/29 , C12N15/82 , C12P7/64 , A61K31/23 , A01H1/04
CPC分类号： A23D9/00 , C07K14/415 , C12N15/8247 , C12N9/16 , C12P7/6463 , C12P7/6472
摘要： By this invention, further properties and uses of plant medium-chain thioesterases are provided. In a first embodiment, this invention relates to plant seed and oil derived from that seed, which normally do not contain laurate, but now are found to contain laurate. In yet a different embodiment, this invention relates to a particular medium-chain thioesterase sequence, the Bay medium-chain thioesterase DNA sequence and to DNA constructs for the expression of this enzyme in a host cell. Other aspects of this invention relate to methods for using a plant medium-chain thioesterase. Expression of a plant medium-chain thioesterase in a bacterial cell to produce medium-chain fatty acids is provided. Methods to produce an unsaturated medium-chain thioesterase by the use of a plant medium-chain thioesterase are also described herein.
摘要翻译： PCT No.PCT / US92 / 04332 Sec。 371日期：1993年11月18日 102（e）1993年11月18日日期PCT 1992年5月21日提交PCT提供了植物中链硫酯酶的进一步的性质和用途。 在第一个实施方案中，本发明涉及来自该种子的植物种子和油，其通常不含月桂酸酯，但现在发现含有月桂酸酯。 在另一个不同的实施方案中，本发明涉及特定的中链硫酯酶序列，Bay中链硫酯酶DNA序列和用于在宿主细胞中表达该酶的DNA构建体。 本发明的其它方面涉及使用植物中链硫酯酶的方法。 提供植物中链硫酯酶在细菌细胞中的表达以产生中链脂肪酸。 本文还描述了通过使用植物中链硫酯酶生产不饱和中链硫酯酶的方法。
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公开(公告)号：US5968791A
公开(公告)日：1999-10-19
申请号：US458109
申请日：1995-06-01
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This invention relates to plant LPAATs, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, and methods to obtain, make and/or use such plant LPAATs. Purification, especially the removal of plant membranes and the substantial separation away from other plant proteins, and use of the plant LPAAT is provided, including the use of the protein as a tool in gene isolation for biotechnological applications. In addition, nucleic acid sequences encoding LPAAT protein regions are provided, and uses of such sequences for isolation of LPAAT genes from plants and for modification of plant triglyceride compositions are described.
摘要翻译： 本发明涉及植物LPAAT，鉴定此类蛋白质的方法，与此类蛋白质相关的氨基酸和核酸序列，以及获得，制备和/或使用此类植物LPAAT的方法。 净化，特别是植物膜的去除以及远离其它植物蛋白质的大量分离，并且提供了植物LPAAT的使用，包括使用蛋白质作为用于生物技术应用的基因分离的工具。 另外，提供了编码LPAAT蛋白质区域的核酸序列，并描述了这些序列用于从植物中分离LPAAT基因和修饰植物甘油三酯组合物的用途。
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7.发明授权Plant thioesterases and use for modification of fatty acid composition in plant seed oils 失效植物硫酯酶，用于植物种子油中脂肪酸组成的改性
公开(公告)号：US5807893A
公开(公告)日：1998-09-15
申请号：US469203
申请日：1995-06-06
CPC分类号： C12N15/8247 , C12N9/16
摘要： By this invention, further properties and uses of plant medium chain thioesterases are provided. In particular, it is now seen that plant seed which would not normally contain medium-chain fatty acid, either as free fatty acids or incorporated into triglyceride molecules, can be found to contain such medium-chain fatty acids. By seed which would not normally contain medium-chain fatty acid is meant seed which contains less than 0.1 mole percent of a given medium-chain fatty acid in total fatty acids. Thus, any plant seed containing a minimum of 1.0 mole percent of a given medium-chain fatty acid in total fatty acids is significantly modified. In one embodiment, this invention relates to plant seed and oil derived from that seed, which normally do not contain laurate, but now are found to contain laurate.
摘要翻译： 通过本发明，提供了植物中链硫酯酶的进一步的性质和用途。 特别地，现在可以看出，通常不含有中链脂肪酸（作为游离脂肪酸或掺入甘油三酯分子）的植物种子可以被发现含有这种中链脂肪酸。 通常不含中链脂肪酸的种子是指在总脂肪酸中含有小于0.1摩尔％的给定中链脂肪酸的种子。 因此，在总脂肪酸中含有至少1.0摩尔％的给定中链脂肪酸的任何植物种子被显着地修饰。 在一个实施方案中，本发明涉及来自该种子的植物种子和油，其通常不含有月桂酸酯，但现在发现含有月桂酸酯。
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公开(公告)号：US5667997A
公开(公告)日：1997-09-16
申请号：US424406
申请日：1995-07-05
IPC分类号： A01H5/00 , A01H5/10 , A23D9/00 , C07K14/415 , C11B1/00 , C11C3/00 , C12N5/00 , C12N5/10 , C12N9/16 , C12N15/09 , C12N15/29 , C12N15/55 , C12N15/82 , C12P7/64 , C12R1/91 , C12N15/63
CPC分类号： A23D9/00 , C07K14/415 , C12N15/8247 , C12N9/16 , C12P7/6418
摘要： By this invention, further plant medium-chain acyl-ACP thioesterases are provided, as well as uses of long-chain thioesterase sequences in conjunction with medium-chain thioesterase sequences. In a first embodiment, this invention relates to particular medium-chain thioesterase sequences from elm and Cuphea, and to DNA constructs for the expression of these thioesterases in host cells for production of C8 and C10 fatty acids. Other aspects of this invention relate to methods for using plant medium-chain thioesterases or medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells. As a further aspect, uses of long-chain thioesterase sequences for anti-sense methods in plant cells in conjunction with expression of medium-chain thioesterases in plant cells is described.
摘要翻译： PCT No.PCT / US93 / 10814 Sec。 371日期：1995年7月5日 102（e）日期1995年7月5日PCT提交1993年10月29日PCT公布。 公开号WO94 / 10288 日本1994年5月11日本发明提供了另外的植物中链酰基-ACP硫酯酶，以及长链硫酯酶序列与中链硫酯酶序列的用途。 在第一个实施方案中，本发明涉及来自elm和Cuphea的特定中链硫酯酶序列，以及用于在宿主细胞中表达这些硫酯酶以生产C8和C10脂肪酸的DNA构建体。 本发明的其它方面涉及使用植物中链硫酯酶或来自非植物来源的中链硫酯酶在植物细胞中提供中链脂肪酸的方法。 作为另一方面，描述了长链硫酯酶序列在植物细胞中用于反义方法与植物细胞中中链硫酯酶表达的用途。
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