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    System and Method of Modular Cloning
    1.
    发明公开
    System and Method of Modular Cloning 审中-公开

    公开(公告)号：US20230257757A1

    公开(公告)日：2023-08-17

    申请号：US18150272

    申请日：2023-01-05

    申请人： Icon Genetics GmbH

    发明人： Ernst Weber ,  Stefan Werner ,  Carola Engler ,  Ramona Grutzner ,  Sylvestre Marillonnet

    IPC分类号： C12N15/66 ,  C12N15/10 ,  C40B40/10

    CPC分类号： C12N15/66 ,  C12N15/1093 ,  C40B40/10

    摘要： System for producing a nucleic acid construct of interest, said system comprising:



    a set of n entry DNAs numbered 1 to n, n being an integer of at least 2,
    each of said n entry DNAs comprising in this order:
    (i) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof;
    (ii) a sequence portion linking the cleavage site of said recognition site of item (i) with the cleavage site of the recognition site of the following item (iii), and
    (iii) a cleavage site of a further type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site;

    the cleavage sites of the type IIs restriction endonuclease recognition sites of item (iii) of entry DNAs 1 to n−1 are complementary to the cleavage sites of the type IIs restriction endonuclease recognition sites of item (i) of entry DNAs 2 to n, respectively;
    the cleavage site of the type IIs restriction endonuclease recognition site of item (iii) of entry DNA n is complementary to the cleavage site of the type IIs restriction endonuclease recognition site of item (i) of entry DNA 1 for allowing annealing of complementary single-stranded overhangs formed by restriction at recognition site (i) of entry DNA 1 and at recognition site (iii) of entry DNA n;
    said system further comprising a destination vector comprising in this order:
    (I) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof;
    (II) a vector backbone preferably comprising a selectable marker gene, said vector backbone linking the cleavage sites of said recognition sites of items (I) and the following item (III);
    (III) a further cleavage site of a type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site, and
    (IV) optionally, an insert between the recognition sites of item (III) and item (I);
    said cleavage sites of items (I) and (III) being different and non-complementary, said recognition sites of items (I) and (III) being preferably recognitions sites of the same endonuclease.

    METHODS OF MODULATING N-GLYCOSYLATION SITE OCCUPANCY OF PLANT-PRODUCED GLYCOPROTEINS AND RECOMBINANT GLYCOPROTEINS
    3.
    发明申请
    METHODS OF MODULATING N-GLYCOSYLATION SITE OCCUPANCY OF PLANT-PRODUCED GLYCOPROTEINS AND RECOMBINANT GLYCOPROTEINS 审中-公开
    调制植物生产的糖蛋白和重组蛋白糖蛋白的N-糖苷化位点的方法

    公开(公告)号：US20160115498A1

    公开(公告)日：2016-04-28

    申请号：US14895350

    申请日：2014-06-03

    申请人： ICON GENETICS GMBH

    发明人： Franziska Jarczowski ,  Romy Kandzia ,  Frank Thieme ,  Victor Klimyuk ,  Yuri Gleba

    IPC分类号： C12N15/82 ,  C12N9/10 ,  C07K16/40

    CPC分类号： C12N15/8257 ,  C07K16/2887 ,  C07K16/32 ,  C07K2317/41 ,  C12N9/1051 ,  C12N15/8258 ,  C12Y204/01

    摘要： A process of producing a recombinant glycoprotein in a plant, in cells of a plant, or in plant cells, comprising expressing in said plant, in cells of said plant or in said plant cells a nucleic acid sequence encoding a polypeptide, said polypeptide having an N-glycosylation site of consensus sequence Asn-X-Ser or Asn-X-Thr, X being any standard amino acid residue, wherein, if the Asn residue of said N-glycosylation site is assigned amino acid sequence position 0, (a) the amino acid residue at position +3 is selected from Thr, Ser, Gly, Leu, Ile, Val and Met; or (b) the amino acid residue at position −1 is selected from Glu and Asp.

    摘要翻译： 一种在植物，植物细胞或植物细胞中产生重组糖蛋白的方法，包括在所述植物的细胞中或在所述植物细胞中表达编码多肽的核酸序列，所述多肽具有 共有序列Asn-X-Ser或Asn-X-Thr的N-糖基化位点，X是任何标准氨基酸残基，其中如果所述N-糖基化位点的Asn残基被指定为氨基酸序列位置0，（a） 位置+3处的氨基酸残基选自Thr，Ser，Gly，Leu，Ile，Val和Met; 或（b）位置-1处的氨基酸残基选自Glu和Asp。

    SYSTEM AND METHOD OF MODULAR CLONING
    9.
    发明申请
    SYSTEM AND METHOD OF MODULAR CLONING 审中-公开

    公开(公告)号：US20170369889A1

    公开(公告)日：2017-12-28

    申请号：US15634803

    申请日：2017-06-27

    申请人： Icon Genetics GmbH

    发明人： Ernst Weber ,  Stefan Werner ,  Carola Engler ,  Ramona Grutzner ,  Sylvestre Marillonnet

    IPC分类号： C12N15/66 ,  C12N15/10

    CPC分类号： C12N15/66 ,  C12N15/1093

    摘要： System for producing a nucleic acid construct of interest, said system comprising: a set of n entry DNAs numbered 1 to n, n being an integer of at least 2, each of said n entry DNAs comprising in this order: (i) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof; (ii) a sequence portion linking the cleavage site of said recognition site of item (i) with the cleavage site of the recognition site of the following item (iii), and (iii) a cleavage site of a further type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site; the cleavage sites of the type IIs restriction endonuclease recognition sites of item (iii) of entry DNAs 1 to n−1 are complementary to the cleavage sites of the type IIs restriction endonuclease recognition sites of item (i) of entry DNAs 2 to n, respectively; the cleavage site of the type IIs restriction endonuclease recognition site of item (iii) of entry DNA n is complementary to the cleavage site of the type IIs restriction endonuclease recognition site of item (i) of entry DNA 1 for allowing annealing of complementary single-stranded overhangs formed by restriction at recognition site (i) of entry DNA 1 and at recognition site (iii) of entry DNA n; said system further comprising a destination vector comprising in this order: (I) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof; (II) a vector backbone preferably comprising a selectable marker gene, said vector backbone linking the cleavage sites of said recognition sites of items (I) and the following item (III); (III) a further cleavage site of a type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site, and (IV) optionally, an insert between the recognition sites of item (III) and item (I); said cleavage sites of items (I) and (III) being different and non-complementary, said recognition sites of items (I) and (III) being preferably recognitions sites of the same endonuclease.

    NICOTIANA BENTHAMIANA PLANTS DEFICIENT IN FUCOSYLTRANSFERASE ACTIVITY
    10.
    发明申请
    NICOTIANA BENTHAMIANA PLANTS DEFICIENT IN FUCOSYLTRANSFERASE ACTIVITY 审中-公开
    尼古丁BENTHAMIANA植物缺乏在FUCOSYLTRANSFERASE活动

    公开(公告)号：US20150080553A1

    公开(公告)日：2015-03-19

    申请号：US14347752

    申请日：2012-10-04

    申请人： ICON GENETICS GMBH

    发明人： Koen Weterings ,  Gerben Van Eldik

    IPC分类号： C12P21/00 ,  C07K16/00 ,  C12N15/82

    CPC分类号： C12P21/005 ,  C07K16/00 ,  C12N9/1051 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/8257 ,  C12Y204/01214

    摘要： The invention provides methods for reducing the levels of alfa (1,3)-fucosylated N-glycans on glycoproteins produced in plants or plant cells. In addition, the invention provides alfa(1,3)-fucosyltransferase genes from Nicotiana benthamiana, and mutant N. benthamiana plants in which the levels of alfa(1,3)-fucosylated N-glycans are reduced.

    摘要翻译： 本发明提供降低在植物或植物细胞中产生的糖蛋白上的α（1,3） - 岩藻糖基化N-聚糖水平的方法。 此外，本发明提供了本发明烟草属的本发明烟草（1,3） - 岩藻糖基转移酶基因和其中α（1,3） - 岩藻糖基化N-聚糖水平降低的突变体本氏烟草植物。