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    Enzymatic cycling assays for homocysteine and cystathionine
    2.
    发明授权
    Enzymatic cycling assays for homocysteine and cystathionine 有权
    同型半胱氨酸和胱硫醚的酶循环试验

    公开(公告)号：US06867014B2

    公开(公告)日：2005-03-15

    申请号：US10651183

    申请日：2003-08-28

    申请人： Glenn Kawasaki ,  Heather Kay Webb ,  Jeffrey Owens ,  Raymond Liedtke ,  Doreen Forest ,  Mark Legaz ,  Sobomabo Lawson

    发明人： Glenn Kawasaki ,  Heather Kay Webb ,  Jeffrey Owens ,  Raymond Liedtke ,  Doreen Forest ,  Mark Legaz ,  Sobomabo Lawson

    IPC分类号： C12Q1/00 ,  C12N9/00 ,  C12N15/52 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/527 ,  G01N33/53 ,  G01N33/68 ,  C12Q1/34 ,  C12Q1/48

    CPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/527 ,  G01N33/6815 ,  Y10S435/968 ,  Y10S435/975

    摘要： The present invention provides an enzymatic cycling assay for assessing the amount of homocysteine and/or cystathionine in a solution such as blood, blood derivatives, or urine. The assay comprises the steps of contacting the solution containing homocysteine and/or cystathionine to form a reaction mixture, with CBS, or a derivative thereof, L-serine, and CBL, or a derivative thereof, for a time period sufficient to catalyze the cyclical conversion of homocysteine form to cystathionine and the reconversion of cystathionine to homocysteine with the production of pyruvate and ammonia; determining the amount of homocysteine and/or ammonia present in the reaction mixture; and determining the amount of homocysteine and/or cystathionine present in the solution based on the amount of pyruvate and/or ammonia formed. Expression vectors and isolation procedures for CBS, or derivatives thereof, and CBL, or derivatives thereof, are also provided as well as test kits for carrying out the assay. In preferred embodiments, the assays can be conducted in 15 minutes or less, with a minimum of enzyme usage.

    摘要翻译： 本发明提供了用于评估溶液如血液，血液衍生物或尿液中高半胱氨酸和/或胱硫醚的量的酶循环测定。 该测定包括以下步骤：使含有同型半胱氨酸和/或胱硫醚的溶液与CBS或其衍生物，L-丝氨酸和CBL或其衍生物反应一段足以催化周期性 同型半胱氨酸形式转化为胱硫醚，并产生丙酮酸和氨，将胱硫醚转化为同型半胱氨酸; 测定反应混合物中存在的同型半胱氨酸和/或氨的量; 并且基于形成的丙酮酸盐和/或氨的量确定溶液中存在的同型半胱氨酸和/或胱硫醚的量。 还提供用于CBS或其衍生物，CBL或其衍生物的表达载体和分离程序以及用于进行测定的测试试剂盒。 在优选的实施方案中，测定可以在15分钟或更短时间内进行，酶用量最小。

    Enzymatic cycling assays for homocysteine and cystathionine
    3.
    发明授权
    Enzymatic cycling assays for homocysteine and cystathionine 有权
    同型半胱氨酸和胱硫醚的酶循环试验

    公开(公告)号：US06664073B1

    公开(公告)日：2003-12-16

    申请号：US09704036

    申请日：2000-11-01

    申请人： Glenn Kawasaki ,  Heather Kay Webb ,  Jeffrey Owens ,  Raymond Liedtke ,  Doreen Forest ,  Mark Legaz ,  Sobomabo Lawson

    发明人： Glenn Kawasaki ,  Heather Kay Webb ,  Jeffrey Owens ,  Raymond Liedtke ,  Doreen Forest ,  Mark Legaz ,  Sobomabo Lawson

    IPC分类号： C12Q126

    CPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/527 ,  Y10S435/968 ,  Y10S435/975

    摘要： The present invention provides an enzymatic cycling assay for assessing the amount of homocysteine and/or cystathionine in a solution such as blood, blood, derivatives, or urine. The assay comprises the steps of contacting the solution containing homocysteine and/or cystathionine to form a reaction mixture, with CBS, or a derivative thereof, L-serine, and CBL, or a derivative thereof, for a time period sufficient to catalyze the cyclical conversion of homocysteine form to cystathionine and the reconversion of cystathionine to homocysteine with the production of pyruvate and ammonia; determining the amount of homocysteine and/or ammonia present in the reaction mixture; and determining the amount of homocysteine and/or cystathionine present in the solution based on the amount of pyruvate and/or ammonia formed. Expression vectors and isolation procedures for CBS, or derivatives thereof, and CBL, or derivatives thereof, are also provided as well as test kits for carrying out the assay. In preferred embodiments, the assays can be conducted in 15 minutes or less, with a minimum of enzyme usage.

    摘要翻译： 本发明提供了用于评估溶液如血液，血液，衍生物或尿液中的同型半胱氨酸和/或胱硫醚的量的酶循环试验。 该测定包括以下步骤：使含有同型半胱氨酸和/或胱硫醚的溶液与CBS或其衍生物，L-丝氨酸和CBL或其衍生物反应一段足以催化周期性 同型半胱氨酸形式转化为胱硫醚，并产生丙酮酸和氨，将胱硫醚转化为同型半胱氨酸; 确定反应混合物中存在的同型半胱氨酸和/或氨的量; 并且基于形成的丙酮酸盐和/或氨的量确定溶液中存在的同型半胱氨酸和/或胱硫醚的量。 还提供用于CBS或其衍生物，CBL或其衍生物的表达载体和分离程序以及用于进行测定的测试试剂盒。 在优选的实施方案中，测定可以在15分钟或更短时间内进行，酶用量最小。

    MAXQ microcontroller
    5.
    发明申请
    MAXQ microcontroller 有权
    MAXQ微控制器

    公开(公告)号：US20060206690A1

    公开(公告)日：2006-09-14

    申请号：US11436801

    申请日：2006-05-18

    申请人： Jeffrey Owens ,  Edward Ma ,  Don Loomis ,  Tom Chenot

    发明人： Jeffrey Owens ,  Edward Ma ,  Don Loomis ,  Tom Chenot

    IPC分类号： G06F9/30

    CPC分类号： G06F9/3824 ,  G06F9/3001 ,  G06F9/30032 ,  G06F9/30043 ,  G06F9/30101 ,  G06F9/30112 ,  G06F9/30134 ,  G06F9/30138 ,  G06F9/30145 ,  G06F9/30163 ,  G06F9/30167 ,  G06F9/30181 ,  G06F9/30185 ,  G06F9/342

    摘要： A microcontroller includes a program memory, data memory, central processing unit, at least one register module, a memory management unit, and a transport network. Instructions are executed in one clock cycle via an instruction word. The instruction word indicates the source module from which data is to be retrieved and the destination module to which data is to be stored. The address/data capability of an instruction word may be extended via a prefix module. If an operation is performed on the data, the source module or the destination module may perform the operation during the same clock cycle in which the data is transferred.

    摘要翻译： 微控制器包括程序存储器，数据存储器，中央处理单元，至少一个寄存器模块，存储器管理单元和传输网络。 指令通过指令字在一个时钟周期内执行。 指令字表示要从中检索数据的源模块和要存储数据的目标模块。 指令字的地址/数据能力可以通过前缀模块来扩展。 如果对数据执行操作，则源模块或目标模块可以在传送数据的相同时钟周期内执行操作。

    Electromyography system
    7.
    发明申请
    Electromyography system 有权
    电子照相系统

    公开(公告)号：US20080064977A1

    公开(公告)日：2008-03-13

    申请号：US11982238

    申请日：2007-10-31

    申请人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    发明人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    IPC分类号： A61B5/05 ,  A61B5/04

    CPC分类号： A61B5/04001 ,  A61B5/0488 ,  A61B5/0492 ,  A61B5/4836 ,  A61B5/4893 ,  A61B17/00234 ,  A61B2017/00022 ,  A61B2017/00039 ,  A61N1/08

    摘要： Systems for determining structural integrity of a bone within the spine of a patient, the bone having a first aspect and a second aspect, wherein the second aspect separated from the first aspect by a width and located adjacent to a spinal nerve. A stimulator is configured to generate an electrical stimulus to be applied to the first aspect of the bone. A monitor is configured to electrically monitor a muscle myotome associated with the spinal nerve to detect if an onset neuro-muscular response occurs in response to the application of the electrical stimulus to the first aspect of the bone. An adjuster is configured to automatically increase the magnitude of the electrical stimulus to until the onset neuro-muscular response is detected. Lastly, a communicator is configured to communicate to a user via at least one of visual and audible means information representing the magnitude of the electrical stimulus which caused the onset neuro-muscular response.

    摘要翻译： 用于确定患者脊柱内骨骼的结构完整性的系统，所述骨具有第一方面和第二方面，其中所述第二方面与所述第一方面分开宽度并且位于脊神经附近。 刺激器被配置为产生要施加到骨的第一方面的电刺激。 监视器被配置为电监测与脊髓神经相关联的肌肉肌肉移植片，以检测是否发生神经 - 肌肉反应发生，以响应于对骨的第一方面的电刺激的应用。 调节器被配置为自动地增加电刺激的大小，直到发现神经肌肉反应被检测到。 最后，通信器被配置为经由视觉和听觉装置中的至少一个与用户通信，该信息表示引起神经肌肉反应发作的电刺激的大小。

    Strap silencer
    8.
    发明申请
    Strap silencer 失效
    表带消声器

    公开(公告)号：US20060283659A1

    公开(公告)日：2006-12-21

    申请号：US11155132

    申请日：2005-06-16

    申请人： Jeffrey Owens ,  Shantelle Owens ,  Donald Miller ,  Susan Miller

    发明人： Jeffrey Owens ,  Shantelle Owens ,  Donald Miller ,  Susan Miller

    IPC分类号： F16F7/00 ,  F16F15/00

    CPC分类号： F16F7/006 ,  F16F7/00

    摘要： There is a noise reduction device for reducing noise from a vibrating strap. There is a securing member including a strip of material having a first surface including hook material and a second surface including loop material, wherein the securing member is configured to removably attach to the vibrating strap by wrapping about the strap and attaching a portion of the first surface to a portion of the second surface. There is a damping member including a plurality of strands of fibers configured to dampen vibratory motion. There is an internal coupling member configured to couple the securing member to the damping member and configured to bundle the plurality of strands of fibers.

    摘要翻译： 有一个降噪装置，用于减少振动带的噪音。 存在包括材料条的固定构件，其具有包括钩材料的第一表面和包括环材料的第二表面，其中所述固定构件构造成通过围绕所述带缠绕并附接所述第一部分的一部分而可移除地附接到所述振动带 表面到第二表面的一部分。 存在包括被配置为抑制振动的多束纤维的阻尼构件。 存在内部联接构件，其构造成将固定构件联接到阻尼构件并且构造成捆扎多束纤维束。

    Electromyography system
    9.
    发明申请
    Electromyography system 审中-公开
    电子照相系统

    公开(公告)号：US20080071191A1

    公开(公告)日：2008-03-20

    申请号：US11981893

    申请日：2007-10-31

    申请人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    发明人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    IPC分类号： A61B5/05 ,  A61B5/04

    CPC分类号： A61B5/04001 ,  A61B5/0488 ,  A61B5/0492 ,  A61B5/4836 ,  A61B5/4893 ,  A61B17/00234 ,  A61B2017/00022 ,  A61B2017/00039 ,  A61N1/08

    摘要： A method of determining relative neuro-muscular response onset value thresholds for a plurality of spinal nerves, comprising: depolarizing a portion of the patient's cauda equina; and measuring the current intensity level at which a neuro-muscular response to the depolarization of the cauda equina is detected in each of the plurality of spinal nerves. A method for detecting the presence of a nerve adjacent the distal end of at least one probe, comprising: determining relative neuro-muscular response onset values for a plurality of spinal nerves; emitting a stimulus pulse from a probe or surgical tool; detecting neuro-muscular responses to the stimulus pulse in each of the plurality of spinal nerves; and concluding that the electrode disposed on the distal end of the at least one probe is positioned adjacent to a first spinal nerve when the neuro-muscular response detected in the first spinal nerve is detected as a current intensity level less than or equal to a corresponding neuro-muscular response onset value of the first spinal nerve.

    摘要翻译： 一种确定多个脊神经的相对神经 - 肌肉反应起始值阈值的方法，包括：使所述患者马尾的一部分去极化; 并且测量在所述多个脊神经的每一个中检测到所述马尾神经去极化的神经肌肉反应的当前强度水平。 一种用于检测与至少一个探针的远端相邻的神经的存在的方法，包括：确定多个脊神经的相对神经 - 肌肉反应起始值; 从探头或手术工具发出刺激脉冲; 检测所述多个脊神经中的每一个中的刺激脉冲的神经 - 肌肉反应; 并且得出当在第一脊神经中检测到的神经肌肉反应被检测为当前强度水平小于或等于相应的第一脊神经时，设置在所述至少一个探针的远端上的电极定位成与第一脊神经相邻 神经肌肉反应起始值第一脊髓神经。

    Electromyography system
    10.
    发明申请
    Electromyography system 有权
    电子照相系统

    公开(公告)号：US20080064976A1

    公开(公告)日：2008-03-13

    申请号：US11981889

    申请日：2007-10-31

    申请人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    发明人： Brian Kelleher ,  James Marino ,  Corbett Stone ,  Robin Vaughn ,  Jeffrey Owens

    IPC分类号： A61N1/05

    CPC分类号： A61B5/04001 ,  A61B5/0488 ,  A61B5/0492 ,  A61B5/4836 ,  A61B5/4893 ,  A61B17/00234 ,  A61B2017/00022 ,  A61B2017/00039 ,  A61N1/08

    摘要： Methods for determining structural integrity of a bone within the spine of a patient, the bone having a first aspect and a second aspect, wherein the second aspect separated from the first aspect by a width and located adjacent to a spinal nerve. The methods involve (a) applying an electrical stimulus to the first aspect of the bone; (b) electrically monitoring a muscle myotome associated with the spinal nerve to detect if an onset neuro-muscular response occurs in response to the application of the electrical stimulus to the first aspect of the bone; (c) automatically increasing the magnitude of the electrical stimulus to until the onset neuro-muscular response is detected; and (d) communicating to a user via at least one of visual and audible means information representing the magnitude of the electrical stimulus which caused the onset neuro-muscular response.

    摘要翻译： 用于确定患者脊柱内骨骼的结构完整性的方法，所述骨具有第一方面和第二方面，其中所述第二方面与所述第一方面分开宽度并且位于脊神经附近。 所述方法包括（a）对骨骼的第一方面施加电刺激; （b）电性监测与脊髓神经相关的肌肉肌肉注射器，以检测是否发生神经 - 肌肉反应，以响应于电刺激对骨的第一方面的应用; （c）自动增加电刺激的大小，直到发现神经肌肉反应发生; 以及（d）通过视觉和听觉手段中的至少一个与用户通信，该信息表示导致发作神经 - 肌肉反应的电刺激的大小。