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21 条记录 (耗时 0.024 秒)

    HEPATOCYTE GROWTH FACTOR FRAGMENTS THAT FUNCTION AS POTENT MET RECEPTOR AGONISTS AND ANTAGONISTS
    1.
    发明申请
    HEPATOCYTE GROWTH FACTOR FRAGMENTS THAT FUNCTION AS POTENT MET RECEPTOR AGONISTS AND ANTAGONISTS 有权
    HEPATOCYTE生长因子片段功能作为MET受体激动剂和拮抗剂

    公开(公告)号：US20130171068A1

    公开(公告)日：2013-07-04

    申请号：US13636053

    申请日：2011-03-21

    申请人： Jennifer R. Cochran ,  Douglas S. Jones, II ,  Ping-Chuan Tsai

    发明人： Jennifer R. Cochran ,  Douglas S. Jones, II ,  Ping-Chuan Tsai

    IPC分类号： C07K14/475

    CPC分类号： C07K14/4753 ,  A61K38/00

    摘要： The NK1 fragment of hepatocyte growth factor (HGF) binds to and activates the Met receptor, a transmembrane receptor tyrosine kinase that plays a critical role in embryonic development and organ formation. The instant application discloses NK1 variant polypeptides which act as agonists or antagonists of HGF. Further disclosed are covalently linked NK1 variant polypeptides. Many of the disclosed variant polypeptides possess improved stability characteristics.

    摘要翻译： 肝细胞生长因子（HGF）的NK1片段结合并激活Met受体，一种跨膜受体酪氨酸激酶，在胚胎发育和器官形成中起关键作用。 本申请公开了作为HGF的激动剂或拮抗剂的NK1变体多肽。 进一步公开的是共价连接的NK1变体多肽。 所公开的变体多肽中的许多具有改善的稳定性特征。

    Mutant epidermal growth factor polypeptides, nucleic acids, and uses therefor
    4.
    发明授权
    Mutant epidermal growth factor polypeptides, nucleic acids, and uses therefor 有权
    突变表皮生长因子多肽，核酸及其用途

    公开(公告)号：US08247531B2

    公开(公告)日：2012-08-21

    申请号：US11725695

    申请日：2007-03-20

    申请人： Jennifer R. Cochran ,  K. Dane Wittrup

    发明人： Jennifer R. Cochran ,  K. Dane Wittrup

    IPC分类号： C07K14/485 ,  A61K38/18

    CPC分类号： C07K14/485 ,  A61K38/00

    摘要： The present invention is based, in part, on our discovery that EGF can be engineered to generate mutants that bind to the EGF receptor (EGFR) of a cell and that have a desirable effect on the activity of the cell. For example, the mutants can agonize the receptor (i.e., increase a biological activity of the receptor), or antagonize the receptor (i.e., decrease or inhibit a biological activity of the receptor). In turn, the rate at which the cell proliferates, for example, can be changed. Moreover, some of these mutants bind EGFR with a higher affinity than wild-type EGF exhibits. The affinity may increase by about, for example, 2-, 5-, 10-, 15-, 20-, 25-, 30-, 50-, or 100-fold relative to wild-type EGF.

    摘要翻译： 本发明部分基于我们的发现，EGF可以被工程化以产生结合细胞的EGF受体（EGFR）的突变体，并且对细胞的活性具有期望的作用。 例如，突变体可以激动受体（即，增加受体的生物活性）或拮抗受体（即降低或抑制受体的生物活性）。 反过来，细胞增殖的速度例如可以改变。 此外，这些突变体中的一些以比野生型EGF显示更高的亲和力结合EGFR。 相对于野生型EGF，亲和力可以增加约例如2-，5-，10-，15-，20-，25-，30-，50-或100倍。

    Cystine knot peptides binding to alpha IIb beta 3 integrins and methods of use
    7.
    发明授权
    Cystine knot peptides binding to alpha IIb beta 3 integrins and methods of use 有权
    结合αIIbβ3整联蛋白的胱氨酸结结合肽和使用方法

    公开(公告)号：US08778888B2

    公开(公告)日：2014-07-15

    申请号：US12938216

    申请日：2010-11-02

    申请人： Jennifer R. Cochran ,  Adam P. Silverman ,  Mihalis S. Kariolis

    发明人： Jennifer R. Cochran ,  Adam P. Silverman ,  Mihalis S. Kariolis

    IPC分类号： C07K14/00 ,  A61K38/16 ,  A61K38/36

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： Disclosed are peptides having a cystine knot structural motif and comprising a sequence engineered for specificity against αIIbβ3 integrin, found on platelets, and a method of using the same in anti-thrombotic therapies. The present peptides utilize a cystine knot scaffold derived from modified agouti-related protein or agatoxin, An alternate library screening strategy was used to isolate variants of peptides that selectively bound to αIIbβ3 integrin or to both αIIbβ3 and αVβ3 integrins. Unique consensus sequences were identified within the identified peptides suggesting alternative molecular recognition events that dictate different integrin binding specificities. In addition, the engineered peptides prevented human platelet aggregation in a plasma-based assay and showed high binding affinity for αIIbβ3 integrin.

    摘要翻译： 公开了具有胱氨酸结结构基序的肽，并且包含在血小板上发现的针对αIIb和bgr3整联蛋白的特异性工程的序列，以及在抗血栓形成治疗中使用该序列的方法。 本发明的肽利用衍生自修饰的agouti相关蛋白或恶性毒素的胱氨酸结支架。使用替代文库筛选策略来分离选择性结合αIIb和bgr3整合素或αIIb和bgr 3和αV和bgr 3整合素的肽的变体。 在鉴定的肽中鉴定出独特的共有序列，表明替代的分子识别事件决定了不同的整联蛋白结合特异性。 此外，工程化肽可防止基于血浆的测定中的人血小板聚集，并显示出对αIIb和bgr3整合素的高结合亲和力。

    Surface modification of interpenetrating polymer networks
    9.
    发明授权
    Surface modification of interpenetrating polymer networks 失效
    互穿聚合物网络的表面改性

    公开(公告)号：US08329826B2

    公开(公告)日：2012-12-11

    申请号：US12590288

    申请日：2009-11-05

    申请人： Laura Hartmann ,  Stayce E. Beck ,  Jennifer R Cochran ,  Curtis W Frank

    发明人： Laura Hartmann ,  Stayce E. Beck ,  Jennifer R Cochran ,  Curtis W Frank

    IPC分类号： C08F8/14 ,  C08F8/32

    CPC分类号： C08J3/246 ,  C08G85/004 ,  C08J2300/14

    摘要： Surface modification methods for an interpenetrating polymer network (IPN) hydrogel to provide a basis for cell or tissue attachment are provided. The method involves the activation of functional groups on the surface of the IPN hydrogel. The activated functional groups are then reacted with amine-containing molecules or hydroxyl-containing molecules. The methods (i) can be performed in an aqueous environment and do not require the use of any organic solvent, (ii) do not require UV treatment, thereby avoiding denaturation of the IPN hydrogel or proteins, and/or (iii) can be performed as a one pot reaction.

    摘要翻译： 提供了互穿聚合物网络（IPN）水凝胶的表面改性方法，以提供细胞或组织附着的基础。 该方法涉及IPN水凝胶表面上的官能团的活化。 然后将活化的官能团与含胺分子或含羟基的分子反应。 方法（i）可以在水性环境中进行，不需要使用任何有机溶剂，（ii）不需要UV处理，从而避免IPN水凝胶或蛋白质变性，和/或（iii）可以 作为一锅反应进行。

    CYSTINE KNOT PEPTIDES BINDING TO ALPHA IIb BETA 3 INTEGRINS AND METHODS OF USE
    10.
    发明申请
    CYSTINE KNOT PEPTIDES BINDING TO ALPHA IIb BETA 3 INTEGRINS AND METHODS OF USE 有权
    CYSTINE KNOT PEPDDES与ALPHA结合IIb BETA 3 INTEGRINS AND METHODS OF USE

    公开(公告)号：US20110136740A1

    公开(公告)日：2011-06-09

    申请号：US12938216

    申请日：2010-11-02

    申请人： Jennifer R. Cochran ,  Adam P. Silverman ,  Mihalis S. Kariolis

    发明人： Jennifer R. Cochran ,  Adam P. Silverman ,  Mihalis S. Kariolis

    IPC分类号： A61K38/16 ,  C07K14/00 ,  C07K1/14 ,  A61P7/02

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： Disclosed are peptides having a cystine knot structural motif and comprising a sequence engineered for specificity against αIIbβ3 integrin, found on platelets, and a method of using the same in anti-thrombotic therapies. The present peptides utilize a cystine knot scaffold derived from modified agouti-related protein or agatoxin, An alternate library screening strategy was used to isolate variants of peptides that selectively bound to αIIbβ3 integrin or to both αIIbβ3 and αVβ3 integrins. Unique consensus sequences were identified within the identified peptides suggesting alternative molecular recognition events that dictate different integrin binding specificities. In addition, the engineered peptides prevented human platelet aggregation in a plasma-based assay and showed high binding affinity for αIIbβ3 integrin.

    摘要翻译： 公开了具有胱氨酸结结构基序的肽，并且包含在血小板上发现的针对αIIb和bgr3整联蛋白的特异性工程的序列，以及在抗血栓形成治疗中使用该序列的方法。 本发明的肽利用衍生自修饰的agouti相关蛋白或恶性毒素的胱氨酸结支架。使用替代文库筛选策略来分离选择性结合αIIb和bgr3整合素或αIIb和bgr 3和αV和bgr 3整合素的肽的变体。 在鉴定的肽中鉴定出独特的共有序列，表明替代的分子识别事件决定了不同的整联蛋白结合特异性。 此外，工程化肽可防止基于血浆的测定中的人血小板聚集，并显示出对αIIb和bgr3整合素的高结合亲和力。