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公开(公告)号：US4753775A
公开(公告)日：1988-06-28
申请号：US722373
申请日：1985-04-12
IPC分类号： G01N33/483 , C12M1/34 , C12Q1/00 , G01N33/48 , G01N33/543 , G01N35/10 , G01N33/551 , G01N35/00
CPC分类号： B01L3/021 , G01N33/48 , B01L2300/0636 , B01L3/508 , G01N2035/1055 , Y10S436/808 , Y10S436/809 , Y10T436/2575
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
摘要翻译： 公开了用于样品中分析物的快速检测和（或）纯化的系统和方法。 该系统利用含有捕获试剂的流体容器和具有两个端口的歧管。 将包含设置在流体容器内的分析物的样品经受捕获试剂并通过将容器的开放端插入一个端口中进行处理。 然后将真空，气体，试剂和洗涤液施加到第二端口以实现捕获试剂和分析物之间的快速相互作用，有效洗涤捕获试剂以及检测和/或收集分析物。 该系统和方法提供了一种基于亲和力复合原理的快速安全，简单的自动化过程手段。 在这一类过程中包括免疫测定，核酸杂交测试和亲和力分离。
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公开(公告)号：US4806313A
公开(公告)日：1989-02-21
申请号：US858628
申请日：1986-05-02
IPC分类号： G01N33/48 , G01N35/10 , G01N33/551 , B01L3/00 , G01N35/00
CPC分类号： G01N33/48 , G01N2035/1055
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
摘要翻译： 公开了用于样品中分析物的快速检测和（或）纯化的系统和方法。 该系统利用含有捕获试剂的流体容器和具有两个端口的歧管。 将包含设置在流体容器内的分析物的样品经受捕获试剂并通过将容器的开放端插入一个端口中进行处理。 然后将真空，气体，试剂和洗涤液施加到第二端口以实现捕获试剂和分析物之间的快速相互作用，有效洗涤捕获试剂以及检测和/或收集分析物。 该系统和方法提供了一种基于亲和力复合原理的快速安全，简单的自动化过程手段。 在这一类过程中包括免疫测定，核酸杂交测试和亲和力分离。
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公开(公告)号：US4775635A
公开(公告)日：1988-10-04
申请号：US858630
申请日：1986-05-02
IPC分类号： G01N33/48 , G01N35/10 , G01N33/543
CPC分类号： G01N33/48 , G01N2035/1055 , Y10S436/807 , Y10S436/818 , Y10T436/2575
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
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公开(公告)号：US4549655A
公开(公告)日：1985-10-29
申请号：US558311
申请日：1983-12-05
CPC分类号： B01L3/502 , G01N33/525
摘要： A container for a layered chemical analysis system includes a first, rigid, member and a second, flexible, member. The flexible member is moveable with respect to the rigid member from a first, unstressed, position to a second, stressed, position in which the flexible member is flexed to generate a reaction force acting in a direction substantially normal to stacked layers to thereby urge the same into intimate contact with each other.
摘要翻译： 用于分层化学分析系统的容器包括第一刚性构件和第二柔性构件。 柔性构件可相对于刚性构件从第一，不受应力的位置移动到第二应力位置，在该位置中柔性构件弯曲以产生作用在基本上垂直于堆叠层的方向上的反作用力，从而促使 相同的彼此亲密接触。
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公开(公告)号：US4214993A
公开(公告)日：1980-07-29
申请号：US892507
申请日：1978-04-03
CPC分类号： B01L3/5021 , G01N30/6091
摘要： An apparatus for separating fluids is formed essentially of three pieces which may be nested together to form a stacked array. The three pieces are a cap, an extraction cartridge housing a separating column, and a primary recovery cup. A secondary recovery cup may also be nested onto the lower end of the waste cup. The primary recovery cup has interior flanges for positioning and protecting the bottom end of the column against damage. Each of the elements has an enlarged upper portion forming an exterior step which facilitates mounting in the swinging bucket of a centrifuge. The column itself may comprise particles of a suitable separatory material with the top and the bottom of the particles retained by porous supports.
摘要翻译： 用于分离流体的装置基本上由三个部件形成，其可以嵌套在一起以形成堆叠的阵列。 这三个部件是盖子，一个容纳分离柱的提取盒和一个主回收杯。 二次回收杯也可以嵌套在废杯的下端。 主回收杯具有用于定位和保护柱的底端的内部法兰以防止损坏。 每个元件具有形成外部台阶的扩大的上部部分，其有利于安装在离心机的摆动桶中。 柱本身可以包括合适的分离材料的颗粒，颗粒的顶部和底部由多孔载体保留。
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公开(公告)号：US4190530A
公开(公告)日：1980-02-26
申请号：US892508
申请日：1978-04-03
IPC分类号： B01D11/02 , B01D15/08 , B01J47/06 , B04B5/02 , B04B5/04 , G01N33/483 , G01N35/00 , B01D15/06
CPC分类号： B04B5/0421 , B01D15/18 , G01N2035/00495
摘要： Samples to be analyzed, such as physiological fluids (blood, urine, etc.) may be processed using centrifugal techniques. The sample is centrifugally passed through a separation column to extract the desired component from the aqueous phase. Next, the extracted component is washed free of extraneous materials and then eluted from the separating column by centrifugally passing a suitable solvent therethrough. The aqueous phase and extracted components are collected in separate containers.To facilitate this method, an apparatus is provided that accommodates switching between fluid flow paths in a centrifuge. The centrifuge utilizes a swinging bucket rotor which contains a separating column, the top end of which employs a fluid reservoir for holding the sample to be extracted. Fluids are passed through the separating column (a first flow path) from a central distributor in the centrifuge rotor while in operation. An outer ring of swinging buckets holds two receptacles (the second and third flow paths), one for the aqueous phase and one for the solvent phase containing the sample extract or physiological component. These two receptacles are placed in radial fluidic alignment with the separating column by the technique of reversing the direction of rotation of the rotor, thus switching between two fluid flow paths.
摘要翻译： 可以使用离心技术处理要分析的样品，例如生理液体（血液，尿液等）。 将样品离心通过分离塔，从水相中提取所需组分。 接下来，将提取的组分洗涤不含外来材料，然后通过离心通过合适的溶剂从分离柱中洗脱。 将水相和提取的组分收集在分开的容器中。 为了方便这种方法，提供一种容纳在离心机中的流体流动路径之间切换的装置。 离心机利用摆动式铲斗转子，该转子包含一个分离柱，其顶端采用一个用于保持要抽出的样品的流体储存器。 在操作中，流体从离心机转子中的中心分配器通过分离塔（第一流动路径）。 摆动桶的外圈容纳两个容器（第二和第三流动路径），一个用于水相，一个用于含有样品提取物或生理组分的溶剂相。 这两个容器通过反转转子旋转方向的技术而与分离塔放置成径向流体对准，从而在两个流体流动路径之间切换。
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