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公开(公告)号：US5369011A
公开(公告)日：1994-11-29
申请号：US899824
申请日：1992-06-16
IPC分类号： C12M1/34 , C12M1/26 , C12Q1/24 , G01N33/569 , C12Q1/08
CPC分类号： G01N33/56911 , C12M33/14 , C12M47/02 , C12Q1/24
摘要： This invention relates to a method for collecting, concentrating and detecting microorganisms from difficult-to-separate environmental samples e.g. oil well samples and the like, for the purpose of their analysis or identification; and apparatus for performing the method.
摘要翻译： 本发明涉及用于从难以分离的环境样品中收集，浓缩和检测微生物的方法，例如， 油井样品等，用于分析或鉴定; 以及用于执行该方法的装置。
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公开(公告)号：US4753775A
公开(公告)日：1988-06-28
申请号：US722373
申请日：1985-04-12
IPC分类号： G01N33/483 , C12M1/34 , C12Q1/00 , G01N33/48 , G01N33/543 , G01N35/10 , G01N33/551 , G01N35/00
CPC分类号： B01L3/021 , G01N33/48 , B01L2300/0636 , B01L3/508 , G01N2035/1055 , Y10S436/808 , Y10S436/809 , Y10T436/2575
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
摘要翻译： 公开了用于样品中分析物的快速检测和（或）纯化的系统和方法。 该系统利用含有捕获试剂的流体容器和具有两个端口的歧管。 将包含设置在流体容器内的分析物的样品经受捕获试剂并通过将容器的开放端插入一个端口中进行处理。 然后将真空，气体，试剂和洗涤液施加到第二端口以实现捕获试剂和分析物之间的快速相互作用，有效洗涤捕获试剂以及检测和/或收集分析物。 该系统和方法提供了一种基于亲和力复合原理的快速安全，简单的自动化过程手段。 在这一类过程中包括免疫测定，核酸杂交测试和亲和力分离。
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公开(公告)号：US4806313A
公开(公告)日：1989-02-21
申请号：US858628
申请日：1986-05-02
IPC分类号： G01N33/48 , G01N35/10 , G01N33/551 , B01L3/00 , G01N35/00
CPC分类号： G01N33/48 , G01N2035/1055
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
摘要翻译： 公开了用于样品中分析物的快速检测和（或）纯化的系统和方法。 该系统利用含有捕获试剂的流体容器和具有两个端口的歧管。 将包含设置在流体容器内的分析物的样品经受捕获试剂并通过将容器的开放端插入一个端口中进行处理。 然后将真空，气体，试剂和洗涤液施加到第二端口以实现捕获试剂和分析物之间的快速相互作用，有效洗涤捕获试剂以及检测和/或收集分析物。 该系统和方法提供了一种基于亲和力复合原理的快速安全，简单的自动化过程手段。 在这一类过程中包括免疫测定，核酸杂交测试和亲和力分离。
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公开(公告)号：US4775635A
公开(公告)日：1988-10-04
申请号：US858630
申请日：1986-05-02
IPC分类号： G01N33/48 , G01N35/10 , G01N33/543
CPC分类号： G01N33/48 , G01N2035/1055 , Y10S436/807 , Y10S436/818 , Y10T436/2575
摘要： A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
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