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公开(公告)号：US08597882B2
公开(公告)日：2013-12-03
申请号：US13365419
申请日：2012-02-03
申请人： John Corbett , John Corbett, Sr.
发明人： John Corbett , John Corbett, Sr.
CPC分类号： G01N35/0098 , B01L3/50851 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0803 , B01L2300/1827 , B01L2400/0409 , G01N2035/00366 , G01N2035/0444
摘要： The present invention relates to methods and apparatus for conducting nucleic acid sequencing by pyrosequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilize a first binding partner or selectively immobilize a second binding partner. The method further comprises the steps of binding or immobilizing the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well.
摘要翻译： 本发明涉及通过焦磷酸测序进行核酸测序的方法和装置。 该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对 约束伙伴 该方法还包括以下步骤：将第一或第二结合伴侣结合或固定到支撑表面，并从所述平台外部的点将试剂分配到每个孔中，其中在分配步骤之后，平台被充分旋转，使得任何残留或 从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。
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公开(公告)号：US08921040B2
公开(公告)日：2014-12-30
申请号：US13386834
申请日：2010-07-29
申请人： John Corbett , John Corbett, Sr.
发明人： John Corbett , John Corbett, Sr.
CPC分类号： G01N35/00029 , B01J2219/00326 , B01J2219/0049 , B01J2219/00536 , B01J2219/0061 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00722 , B01L3/50851 , B01L2300/0803 , B01L2300/0864 , B01L2400/0409 , G01N33/5308 , G01N33/582 , G01N35/00069 , G01N2035/00495
摘要： The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilize a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilize a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及进行测定的方法和装置。 特别地，本发明涉及可用于进行测定，特别是多步测定的可旋转平台。 本发明提供了一种可旋转平台，其适于将第一结合伴侣固定在所述平台的表面上的一个或多个离散区域中，或者选择性地将第二结合伴侣固定在所述平台的表面上的一个或多个离散区域中。 本发明还涉及方法，装置，试剂盒以及用于进行测定的可旋转平台的使用。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
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公开(公告)号：US20130203049A1
公开(公告)日：2013-08-08
申请号：US13365419
申请日：2012-02-03
申请人： John Corbett , John Corbett, SR.
发明人： John Corbett , John Corbett, SR.
IPC分类号： G01N33/53
CPC分类号： G01N35/0098 , B01L3/50851 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0803 , B01L2300/1827 , B01L2400/0409 , G01N2035/00366 , G01N2035/0444
摘要： The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及用于进行核酸测序的方法和装置。 该方法包括以下步骤：提供具有至少一个孔的平台，用于容纳至少一个支撑表面，并在每个孔内提供至少一个支撑表面，其中所述支撑表面适于固定第一结合伴侣或选择性地固定第二结合对 约束伙伴 该方法还包括以下步骤：将第一或第二结合配偶体固定或固定到支撑表面，并从所述平台外部的点向试剂分配，其中在分配步骤之后，平台被充分旋转，使得任何残留或 从每个孔和/或每个支撑表面基本上离心地去除未反应的所述试剂，其中在旋转期间每个支撑表面保持在每个孔内。 本发明还涉及用于进行核酸测序的试剂盒和试剂盒的用途。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
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公开(公告)号：US20120282708A1
公开(公告)日：2012-11-08
申请号：US13386834
申请日：2010-07-29
申请人： John Corbett , John Corbett, SR.
发明人： John Corbett , John Corbett, SR.
IPC分类号： G01N33/53
CPC分类号： G01N35/00029 , B01J2219/00326 , B01J2219/0049 , B01J2219/00536 , B01J2219/0061 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00722 , B01L3/50851 , B01L2300/0803 , B01L2300/0864 , B01L2400/0409 , G01N33/5308 , G01N33/582 , G01N35/00069 , G01N2035/00495
摘要： The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
摘要翻译： 本发明涉及进行测定的方法和装置。 特别地，本发明涉及可用于进行测定，特别是多步测定的可旋转平台。 本发明提供了一种可旋转平台，其适于将第一结合伴侣固定在所述平台的表面上的一个或多个离散区域中，或者选择性地将第二结合伴侣固定在所述平台的表面上的一个或多个离散区域中。 本发明还涉及方法，装置，试剂盒以及用于进行测定的可旋转平台的使用。 特别地，本发明主要用于通过焦磷酸测序对核酸进行测序，然而本发明不限于该领域。
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