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公开(公告)号：US20090075333A1
公开(公告)日：2009-03-19
申请号：US12064332
申请日：2006-08-18
摘要： Reduced genome strains of E. coli MGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG155的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US4920686A
公开(公告)日：1990-05-01
申请号：US307099
申请日：1989-02-07
IPC分类号： A01K85/01
CPC分类号： A01K85/01
摘要： A fishing lure incorporating a pocket dimensioned to hold a sinker and/or rattle within the lure body includes an easy access slit formed in the lure material to allow ready exchange of sinker/rattle combinations.
摘要翻译： 钓鱼诱饵包含尺寸适于在诱饵体内保持沉降片和/或拨浪鼓的口袋，包括形成在诱饵材料中的容易进入狭缝，以允许即将更换沉降片/拨浪鼓组合。
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公开(公告)号：US4873783A
公开(公告)日：1989-10-17
申请号：US307823
申请日：1989-02-07
IPC分类号： A01K85/00
CPC分类号： A01K85/00
摘要： A fishing lure including a thin walled pouch for enclosing that portion of a fishing hook which normally extends from a lure body when the lure is threaded on a hook.
摘要翻译： 捕鱼诱饵包括一个薄壁袋，用于封闭钓鱼钩的那部分，当诱饵穿在钩上时，钓鱼钩通常从诱饵体延伸。
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公开(公告)号：US20120219994A1
公开(公告)日：2012-08-30
申请号：US13363848
申请日：2012-02-01
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US08178339B2
公开(公告)日：2012-05-15
申请号：US12064332
申请日：2006-08-18
摘要： Reduced genome strains of E. coli MG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
摘要翻译： 描述了大肠杆菌MG1655的减少的基因组菌株。 在各种实施方案中，与亲本菌株和市售菌株相比，菌株具有相同或改善的生长速率，转化效率，蛋白质表达，DNA产生，DNA产量和/或DNA质量的一种或多种。
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公开(公告)号：US07935251B2
公开(公告)日：2011-05-03
申请号：US12854420
申请日：2010-08-11
申请人： John W. Campbell
发明人： John W. Campbell
IPC分类号： B01D33/70
摘要： A facility for treating solid and liquid waste includes a receiving station, a screening station, a preprocessing station, an alkali mixing station, a press station and a processing station. The screening station is connected to an inlet capable of receiving liquid and solid waste and includes a screen for capturing at least some of the solid waste. The preprocessing station is in fluid communication with the screening station, and includes a degrit chamber for settling out an additional amount of the solids. The alkali mixing station includes an alkali for mixing with the waste to stabilize the waste at a predetermined pH level. The press station receives the stabilized waste and includes a press for separating out an additional portion of the solids. The processing station includes at least one aerobic microorganism generating unit for converting nitrites into nitrogen gas and consuming carbon-based waste material.
摘要翻译： 用于处理固体和液体废物的设备包括接收站，筛选站，预处理站，碱混合站，压力站和处理站。 筛选站连接到能够接收液体和固体废物的入口，并且包括用于捕获至少一些固体废物的筛网。 预处理站与筛选站流体连通，并且包括用于沉淀附加量的固体的除渣室。 碱混合站包括用于与废物混合的碱，以将废物稳定在预定的pH水平。 压力站接收稳定的废物，并包括用于分离附加部分固体的压力机。 处理站包括至少一个用于将亚硝酸盐转化成氮气并消耗碳基废料的好氧微生物产生单元。
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公开(公告)号：US5445952A
公开(公告)日：1995-08-29
申请号：US7559
申请日：1993-01-22
申请人： John W. Campbell , Alex Cheung , Christina K. Eddy
发明人： John W. Campbell , Alex Cheung , Christina K. Eddy
CPC分类号： C12P17/186 , C12N15/52 , C12N15/70
摘要： The present invention relates to a method to enhance a cell's ability to produce biotin precursors and/or biotin by deregulating at least one enzyme of the fatty acid biosynthetic pathway in the cell, preferably an enzyme that carries out an early step in the pathway. Preferably, the biotin biosynthetic pathway is also deregulated. The invention includes biotin-producing cells in which at least one enzyme of the fatty acid biosynthetic pathway is deregulated, preferably by transforming the cells with nucleic acid sequences encoding at least one of those enzymes; methods to produce such cells; and use of such cells to produce biotin.
摘要翻译： 本发明涉及一种增强细胞生产生物素前体和/或生物素的能力的方法，该方法是通过使细胞中的至少一种脂肪酸生物合成途径的酶失调，优选在该途径中进行早期步骤的酶。 优选地，生物素生物合成途径也是失调的。 本发明包括生物素产生细胞，其中至少一种脂肪酸生物合成途径的酶被去调节，优选通过用编码这些酶中的至少一种的核酸序列转化细胞; 生产这种细胞的方法; 并使用这样的细胞产生生物素。
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公开(公告)号：US4214398A
公开(公告)日：1980-07-29
申请号：US846533
申请日：1977-10-28
IPC分类号： A01K83/00
CPC分类号： A01K83/00
摘要： A fishhook having an offset in its shank which acts as a lever to turn the hook as pressure is applied to the line so as to engage the point in the flesh of the fish regardless of the angle at which pressure is applied to the line.
摘要翻译： 具有在其柄部上具有偏移的鱼钩，其作为杠杆以在压力施加到线上时转动钩，以便与鱼的肉接合，而不管施加压力的线的角度如何。
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公开(公告)号：US08765408B2
公开(公告)日：2014-07-01
申请号：US13363848
申请日：2012-02-01
摘要： The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
摘要翻译： 本发明提供了一种具有遗传工程化以小于其天然亲本菌株的基因组的基因组的细菌。 具有较小基因组的细菌可以更有效地产生商业产品。 本发明还提供从细菌基因组中删除基因和其他DNA序列的方法。 该方法提供精确的缺失，很少引入缺失位点周围基因组DNA序列的突变。 因此，该方法可用于在细菌中产生一系列缺失，而不增加基因组内不希望的同源重组的可能性。 此外，本发明提供的一些方法也可用于用期望的DNA序列替代细菌基因组的区域。
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公开(公告)号：US20130011874A1
公开(公告)日：2013-01-10
申请号：US13445672
申请日：2012-04-12
摘要： Reduced genome strains of E. coli MG1 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.
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