公开(公告)号：US09139843B2

公开(公告)日：2015-09-22

申请号：US13478515

申请日：2012-05-23

申请人： Ute Schellenberger ,  Jun-Zhi Wei ,  Genhai Zhu

发明人： Ute Schellenberger ,  Jun-Zhi Wei ,  Genhai Zhu

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07K14/325 ,  A01N25/00 ,  A01H5/10 ,  C12N1/21 ,  C12N15/32 ,  C07K14/66 ,  A01N63/02

CPC分类号： C12N15/8285 ,  A01N63/02 ,  C07K14/325 ,  C07K14/66 ,  Y02A40/164

摘要： The invention provides novel δ-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.

摘要翻译： 本发明提供了从苏云金芽孢杆菌获得的具有针对线虫杀虫活性的新颖的δ-内毒素多肽及其变体和片段。 本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。 这些组合物用于防治害虫，特别是植物寄生线虫的方法。

公开(公告)号：US20130031667A1

公开(公告)日：2013-01-31

申请号：US13541202

申请日：2012-07-03

申请人： Steven D. Gruver ,  Lu Liu ,  Ute Schellenberger ,  Jun-Zhi Wei ,  Wieping Xie ,  Genhai Zhu

发明人： Steven D. Gruver ,  Lu Liu ,  Ute Schellenberger ,  Jun-Zhi Wei ,  Wieping Xie ,  Genhai Zhu

IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/10 ,  C12N15/63 ,  A01H5/00 ,  C12N5/10 ,  C07K14/195

CPC分类号： C07K14/195 ,  A01N37/46 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： The invention provides novel polypeptides, and variants and fragments thereof, having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.

摘要翻译： 本发明提供具有针对线虫杀虫活性的新型多肽及其变体和片段。 本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。 这些组合物用于防治害虫，特别是植物寄生线虫的方法。

公开(公告)号：US20060026708A1

公开(公告)日：2006-02-02

申请号：US11172536

申请日：2005-06-30

申请人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8285 ,  C07K14/415 ,  C12N15/8282 ,  Y02A40/164

摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了保护植物免受病原体，特别是病原真菌或线虫的方法。 还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US08735147B2

公开(公告)日：2014-05-27

申请号：US13439295

申请日：2012-04-04

申请人： Daniel J. Altier ,  Xuehua Hu ,  Daniel L. Siehl ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Daniel J. Altier ,  Xuehua Hu ,  Daniel L. Siehl ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： C12N15/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/8285 ,  A01N37/46 ,  A01N65/00 ,  C07K14/415 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164 ,  A01N65/20

摘要： The invention provides novel cyclotide polypeptides, and variants and fragments thereof, obtained from Butterfly pea and having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant-parasitic pests such as nematodes and insects.

摘要翻译： 本发明提供了从蝴蝶豌豆获得并对线虫具有杀虫活性的新颖的环肽多肽及其变体和片段。 本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。 这些组合物用于防治害虫，特别是植物寄生虫害害虫如线虫和昆虫的方法。

公开(公告)号：US20130029844A1

公开(公告)日：2013-01-31

申请号：US13439295

申请日：2012-04-04

申请人： Daniel J. Altier ,  Xuehua Hu ,  Daniel L. Siehl ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Daniel J. Altier ,  Xuehua Hu ,  Daniel L. Siehl ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： A01N37/18 ,  A01P5/00 ,  C12N15/82 ,  A01C1/06 ,  C07K14/415 ,  C12N15/29

CPC分类号： C12N15/8285 ,  A01N37/46 ,  A01N65/00 ,  C07K14/415 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164 ,  A01N65/20

摘要： The invention provides novel cyclotide polypeptides, and variants and fragments thereof, obtained from Butterfly pea and having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant-parasitic pests such as nematodes and insects.

摘要翻译： 本发明提供了从蝴蝶豌豆获得并对线虫具有杀虫活性的新颖的环肽多肽及其变体和片段。 本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。 这些组合物用于防治害虫，特别是植物寄生虫害害虫如线虫和昆虫的方法。

公开(公告)号：US20080090294A1

公开(公告)日：2008-04-17

申请号：US11874943

申请日：2007-10-19

申请人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8285 ,  C07K14/415 ,  C12N15/8282 ,  Y02A40/164

摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

公开(公告)号：US20080090293A1

公开(公告)日：2008-04-17

申请号：US11874941

申请日：2007-10-19

申请人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8285 ,  C07K14/415 ,  C12N15/8282 ,  Y02A40/164

摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了保护植物免受病原体，特别是病原真菌或线虫的方法。 还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US20080040825A1

公开(公告)日：2008-02-14

申请号：US11874942

申请日：2007-10-19

申请人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Hana Ali ,  Robert Keenan ,  Michael Lassner ,  Mathias Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： A01H5/00 ,  C12N15/00

CPC分类号： C12N15/8285 ,  C07K14/415 ,  C12N15/8282 ,  Y02A40/164

摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

公开(公告)号：US08637739B2

公开(公告)日：2014-01-28

申请号：US13541202

申请日：2012-07-03

申请人： Steven D. Gruver ,  Lu Liu ,  Ute Schellenberger ,  Jun-Zhi Wei ,  Wieping Xie ,  Genhai Zhu

发明人： Steven D. Gruver ,  Lu Liu ,  Ute Schellenberger ,  Jun-Zhi Wei ,  Wieping Xie ,  Genhai Zhu

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/00 ,  C12N15/82

CPC分类号： C07K14/195 ,  A01N37/46 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： The invention provides novel polypeptides, and variants and fragments thereof, having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.

摘要翻译： 本发明提供具有针对线虫杀虫活性的新型多肽及其变体和片段。 本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。 这些组合物用于防治害虫，特别是植物寄生线虫的方法。

公开(公告)号：US07301069B2

公开(公告)日：2007-11-27

申请号：US11172536

申请日：2005-06-30

申请人： Hana S. Ali ,  Robert J. Keenan ,  Michael Lassner ,  Mathias L. Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

发明人： Hana S. Ali ,  Robert J. Keenan ,  Michael Lassner ,  Mathias L. Muller ,  Gowri Shah ,  Jun-Zhi Wei ,  Gusui Wu

IPC分类号： C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8285 ,  C07K14/415 ,  C12N15/8282 ,  Y02A40/164

摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了保护植物免受病原体，特别是病原真菌或线虫的方法。 还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。