Structure and expression of the biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase
    4.
    发明授权
    Structure and expression of the biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase 失效
    异聚乙酰辅酶A羧化酶的生物素羧化酶亚基的结构和表达

    公开(公告)号:US06218600B1

    公开(公告)日:2001-04-17

    申请号:US08662344

    申请日:1996-06-12

    CPC classification number: C12N9/93 C12N15/8243 C12N15/8247

    Abstract: The gene encoding a biotin carboxylase subunit of heteromeric ACCase has been isolated and sequenced. The cDNA sequence and deduced amino acid sequence is set forth in FIG. 1 and has been accorded GenBank Accession No. L38260. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis in plants and seeds which depends on malonyl-CoA in the plastid may also be controlled.

    Abstract translation: 已经分离并测序了编码异聚ACCase的生物素羧化酶亚基的基因。 cDNA序列和推导的氨基酸序列如图1所示。 1,已获得GenBank登录号L38260。 通过控制本发明的基因的表达,可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,通过在正义或反义方向引入本发明基因的构建体,可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,也可以控制依赖于质体中丙二酰辅酶A的植物和种子中的脂肪酸合成。

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