-
公开(公告)号：US20110129924A1
公开(公告)日：2011-06-02
申请号：US12308960
申请日：2006-09-12
IPC分类号： C12N5/071
摘要： Porous polymeric articles, and more specifically, porous polymeric articles for tissue engineering and organ replacement, are described. In some embodiments, methods described herein include use of a polymer-solvent system (e.g., phase inversion) to generate porosity in a structure. The process may include formation of a structure precursor material including a first crosslinkable component and a second component that can be precipitated in a precipitation medium. The structure precursor material may be shaped into a three-dimensional shape by a suitable technique such as three-dimensional printing. Upon shaping of the structure precursor material, at least a portion of the first component may be crosslinked. The structure may then be contacted with a precipitation medium to remove the precursor solvent from the structure, which can cause the second polymer component to precipitate and form a porous structure containing a network of uniform pores. In some embodiments, the porous structure is constructed and arranged for use as a template for ultrafiltration, cell growth, and/or for forming complex, biomimetic, porous biohybrid organs, where living cells can be immobilized and perform their normal physiological functions.
摘要翻译： 描述了多孔聚合物制品，更具体地说，用于组织工程和器官置换的多孔聚合物制品。 在一些实施方案中，本文所述的方法包括使用聚合物 - 溶剂体系（例如相转化）以在结构中产生孔隙。 该方法可以包括形成包含可在沉淀介质中沉淀的第一可交联组分和第二组分的结构前体材料。 结构前体材料可以通过诸如三维印刷之类的合适技术成形为三维形状。 在结构前体材料成型时，第一组分的至少一部分可以是交联的。 然后可以将结构与沉淀介质接触以从结构中除去前体溶剂，这可使第二聚合物组分沉淀并形成含有均匀孔网络的多孔结构。 在一些实施方案中，多孔结构被构造和布置为用作超滤，细胞生长和/或形成复合物，仿生性，多孔生物杂交器官的模板，其中活细胞可被固定并执行其正常的生理功能。
-
2.发明申请THREE DIMENSIONAL FABRICATION OF BIOCOMPATIBLE STRUCTURES IN ANATOMICAL SHAPES AND DIMENSIONS FOR TISSUE ENGINEERING AND ORGAN REPLACEMENT 审中-公开用于组织工程和组织替代的解剖形状和尺寸中生物化学结构的三维制造公开(公告)号：US20100240117A1
公开(公告)日：2010-09-23
申请号：US12749831
申请日：2010-03-30
CPC分类号： A61L27/38 , A61L27/56 , B33Y80/00 , C12N5/0068 , C12N2533/30
摘要： Methods and apparatuses involving biocompatible structures for tissue engineering and organ replacement and, more specifically, biocompatible structures formed by three-dimensional fabrication, are described. In some embodiments, the biocompatible structures are scaffolds for cells that can be used as tissue engineering templates and/or as artificial organs. The structures may be three-dimensional and can mimic the shapes and dimensions of tissues and/or organs, including the microarchitecture and porosities of the tissues and organs. Pores in the structure may allow delivery of molecules across the structure, and may facilitate cell migration and/or generation of connective tissue between the structure and its host environment. Structures of the invention can be implanted into a mammal and/or may be used ex vivo as bioartificial assist devices.
摘要翻译： 描述了涉及用于组织工程和器官置换的生物相容性结构的方法和装置，更具体地，涉及通过三维制造形成的生物相容性结构。 在一些实施方案中，生物相容性结构是可用作组织工程模板和/或作为人造器官的细胞的支架。 结构可以是三维的并且可以模拟组织和/或器官的形状和尺寸，包括组织和器官的微结构和孔隙率。 结构中的孔可以允许跨结构传递分子，并且可以促进细胞迁移和/或结构与其宿主环境之间的结缔组织的产生。 本发明的结构可以植入哺乳动物和/或作为生物人造辅助装置离体使用。
-
公开(公告)号：US07310129B2
公开(公告)日：2007-12-18
申请号：US10948570
申请日：2004-09-24
申请人： Jens Stäcker , Heiko Hommen , Jens Uwe Bruch , Marlene Strobl , Karl Schumacher
发明人： Jens Stäcker , Heiko Hommen , Jens Uwe Bruch , Marlene Strobl , Karl Schumacher
CPC分类号： G03F7/70466 , G03F7/70733
摘要： The mutually associated structure patterns, which are provided on one mask, or a plurality of masks for a double or multiple exposure can be received by the mask substrate holder. The mask substrate holder has two receiving stations one for each of the masks. Alternatively, both structure patterns for the double exposure are formed on one mask. The substrate holder has one receiving station. The substrate holder, is displaced from the section including first structure pattern to the second, between the two exposure operations, without the masks having to be loaded or unloaded, and realigned.
摘要翻译： 提供在一个掩模上的相互关联的结构图案或用于双重或多次曝光的多个掩模可以被掩模基板保持器接收。 掩模基板保持器具有两个用于每个掩模的接收站。 或者，双曝光的两种结构图案都形成在一个掩模上。 基板支架有一个接收台。 在两个曝光操作之间，衬底保持器从包括第一结构图案的部分移动到第二结构图案，而不必将掩模装载或卸载，并重新对准。
-
公开(公告)号：US07248351B2
公开(公告)日：2007-07-24
申请号：US11067191
申请日：2005-02-25
CPC分类号： G03F7/70083 , G01N21/9501 , G01N21/956 , G03F7/70625
摘要： An inspection system includes an illumination source configured to illuminate a blazed phase grating sample, image collection pathways and an imaging system configured to capture an image of a sample point of the blazed phase grating sample, and a controller configured to adjust the illumination source in response to an analysis of the image of the sample point to determine illumination uniformity of the inspection system.
摘要翻译： 检查系统包括：照明源，被配置为照亮闪耀相位光栅样本，图像采集路径和被配置为捕获闪耀相位光栅样本的采样点的图像的成像系统;以及控制器，被配置为响应于调整照明源 对采样点的图像进行分析，以确定检查系统的照明均匀性。
-
5.发明申请Manipulation of chemical and/or biological species using magnetic field modulators 审中-公开使用磁场调制器操纵化学和/或生物物种公开(公告)号：US20070134788A1
公开(公告)日：2007-06-14
申请号：US11297797
申请日：2005-12-08
申请人： Karl Schumacher , Jackie Ying , Jeremy Loh , Shyi Kan
发明人： Karl Schumacher , Jackie Ying , Jeremy Loh , Shyi Kan
CPC分类号： C12N5/0062 , C12N2535/10
摘要： The present invention relates to methods and apparatus for manipulating chemical and/or biological species (e.g., cells) and, more specifically, to methods and apparatus for manipulating chemical and/or biological species using magnetic fields. In one embodiment, a method of manipulating cells involves the patterning of cells using a magnetic field modulator, which can modulate the magnetic field created by a magnetic field source. The cells may be magnetically susceptible in some cases; for instance, they may be tagged with magnetic particles. When a fluid containing cells is brought in contact with a surface, and the magnetic field modulator is positioned proximate the surface, the cells may form a pattern proximate the surface by aligning with portions of the modulated magnetic field. The position of the pattern of cells may be at least partially determined by the position of the magnetic field modulator in relation to the surface. Advantageously, in certain embodiments, the magnetic field modulator is not integrally connected to the surface. Thus, patterns of cells can be formed on various types of surfaces and the magnetic field modulator may be repositioned without altering the surface. In one particular embodiment, moving the magnetic field modulator from a first to a second position enables the formation of a second pattern of cells on the surface. This method can allow the formation of patterns comprising multiple cell types on a single surface. The methods and apparatuses of the present invention can be used in a variety of settings. One such setting involves the patterning of multiple cell types proximate a surface, i.e., for studying cell-cell interactions. Another setting involves the formation of three-dimensional cellular structures (e.g., tissues).
摘要翻译： 本发明涉及用于操纵化学和/或生物物种（例如细胞）的方法和装置，更具体地涉及使用磁场操纵化学和/或生物物种的方法和装置。 在一个实施例中，一种操纵单元的方法涉及使用磁场调制器对单元进行图案化，该磁场调制器可以调制由磁场源产生的磁场。 在某些情况下细胞可能是磁性敏感的; 例如，它们可以被磁性颗粒标记。 当含有细胞的流体与表面接触并且磁场调制器位于表面附近时，细胞可以通过与调制磁场的部分对准而形成靠近表面的图案。 电池图案的位置可以至少部分地由磁场调制器相对于表面的位置决定。 有利地，在某些实施例中，磁场调制器不一体地连接到表面。 因此，可以在各种类型的表面上形成单元的图案，并且可以在不改变表面的情况下重新定位磁场调制器。 在一个特定实施例中，将磁场调制器从第一位置移动到第二位置使得能够在表面上形成第二图案的单元。 该方法可以允许在单个表面上形成包括多个单元格类型的图案。 本发明的方法和装置可以用于多种设置。 一种这样的设置涉及在表面附近形成多种细胞类型，即用于研究细胞 - 细胞相互作用。 另一种设置涉及三维细胞结构（例如，组织）的形成。
-
公开(公告)号：US06343327B2
公开(公告)日：2002-01-29
申请号：US08968649
申请日：1997-11-12
申请人： Edward Paul Daniels, Jr. , John Kwant , Paul Mitchell , John Rahrig , Karl Schumacher , Clare Woodman
发明人： Edward Paul Daniels, Jr. , John Kwant , Paul Mitchell , John Rahrig , Karl Schumacher , Clare Woodman
IPC分类号： G06F1314
CPC分类号： H04L51/066 , G06Q10/107 , H04L51/00
摘要： A printstream processor separates documents, e.g. in a printstream or batch, into a physical delivery printstream and an electronic delivery printstream based on delivery preferences stored in a database. The documents in the physical delivery printstream are printed and combined with physical inserts for physical delivery, e.g. by the U.S. Postal Server. The documents in the electronic delivery printstream are combined with electronic inserts for electronic delivery, for example, via electronic mail, facsimile, pager, or to a server on World Wide Web.
摘要翻译： 打印流处理器分离文档，例如 在打印流或批处理中，基于存储在数据库中的传送偏好，进入物理传送打印流和电子传送打印流。 打印物理传送打印流中的文档，并将其与用于物理传送的物理插入物组合。 由美国邮政服务器。 电子传送打印流中的文档与例如经由电子邮件，传真机，寻呼机或万维网上的服务器的电子插件组合。
-
公开(公告)号：US06332949B1
公开(公告)日：2001-12-25
申请号：US09269633
申请日：1999-03-30
IPC分类号： B01D100
CPC分类号： C01B17/88 , Y10S159/02 , Y10S159/16 , Y10S159/19
摘要： A process for concentrating spent sulphuric acids particularly sulphuric acids which arise during the nitration of hydrocarbons with nitric acid in the presence of sulphuric acid, wherein single- or multi-stage concentration is effected under vacuum.
摘要翻译： 一种用于在硫酸存在下用硝酸硝化烃中产生的废硫酸，特别是硫酸的方法，其中在真空下进行单级或多级浓缩。
-
8.发明授权High throughput cell-based assays fabricated with integrated silicon and cell culture technologies 有权采用集成硅和细胞培养技术制造的高通量基于细胞的测定公开(公告)号：US08003380B2
公开(公告)日：2011-08-23
申请号：US11325609
申请日：2006-01-04
申请人： Shyi Herng Kan , Jackie Y. Ying , Hanry Yu , Karl Schumacher , Susanne San San Ng , Wanxin Sun
发明人： Shyi Herng Kan , Jackie Y. Ying , Hanry Yu , Karl Schumacher , Susanne San San Ng , Wanxin Sun
IPC分类号： C12M1/12
CPC分类号： C12N5/0068 , B01L3/5085 , B01L2300/069 , B01L2300/0829 , B01L2300/0887 , C12M23/20 , C12M23/34 , C12M25/02 , C12N2533/10 , C12N2533/54 , C12N2535/10
摘要： The present invention relates to articles and methods involving porous materials (e.g., membranes) which may interact with species, such as biological molecules, cells, etc., whereby the species may adhere to or become immobilized with respect to a surface of the porous material or an adhesion layer coating the porous surface. The porous material may be capable of attaching species with control over the positioning and spatial distribution of the species across the surface of the material. Such articles and methods may be useful in, for example, biological assays, biological sensors, or in the culturing of biological cells.
摘要翻译： 本发明涉及涉及可与物种如生物分子，细胞等相互作用的多孔材料（例如膜）的制品和方法，由此该物质可能相对于多孔材料的表面粘附或变得固定 或涂覆多孔表面的粘附层。 多孔材料可以能够附着物质以控制物质在材料表面上的定位和空间分布。 这样的制品和方法可用于例如生物测定，生物传感器或生物细胞的培养。
-
9.发明申请Three-dimensional fabrication of biocompatible structures in anatomical shapes and dimensions for tissue engineering and organ replacement 失效用于组织工程和器官置换的解剖学形状和尺寸的生物相容性结构的三维制造公开(公告)号：US20070218544A1
公开(公告)日：2007-09-20
申请号：US11375375
申请日：2006-03-14
CPC分类号： A61L27/38 , A61L27/56 , B33Y80/00 , C12N5/0068 , C12N2533/30
摘要： The present invention relates to methods and apparatuses involving biocompatible structures for tissue engineering and organ replacement and, more specifically, to methods and apparatuses involving biocompatible structures formed by three-dimensional fabrication for tissue engineering and organ replacement. In some embodiments, the biocompatible structures are scaffolds for cells that can be used as tissue engineering templates and/or as artificial organs. The structures may be three-dimensional and can mimic the shapes and dimensions of tissues and/or organs, including the microarchitecture and porosities of the tissues and organs. In some cases, a structure formed by three-dimensional fabrication comprises a wall defining a cavity and a plurality of pores in at least a portion of the wall. The pores may permeate the wall and enable exchange of a component (e.g., a molecule and/or a cell) between a portion interior to the cavity and a portion exterior to the cavity. For instance, pores may allow delivery of molecules, cell migration, and/or generation of connective tissue between the structure and its host environment. Structures of the invention can be implanted into a mammal, or alternatively and/or additionally, can be used ex vivo as bioartificial assist devices.
摘要翻译： 本发明涉及包括用于组织工程和器官置换的生物相容结构的方法和装置，更具体地涉及涉及通过用于组织工程和器官置换的三维制造形成的生物相容性结构的方法和装置。 在一些实施方案中，生物相容性结构是可用作组织工程模板和/或作为人造器官的细胞的支架。 结构可以是三维的并且可以模拟组织和/或器官的形状和尺寸，包括组织和器官的微结构和孔隙率。 在一些情况下，通过三维制造形成的结构包括在壁的至少一部分中限定空腔和多个孔的壁。 孔可以渗透到壁上并且使得能够在空腔内部的部分和腔外部的部分（例如，分子和/或细胞）之间进行交换。 例如，孔可以允许在结构和其宿主环境之间传递分子，细胞迁移和/或结缔组织的产生。 本发明的结构可以植入哺乳动物，或者另外地和/或另外可以作为生物人造辅助装置离体使用。
-
公开(公告)号：US20060193531A1
公开(公告)日：2006-08-31
申请号：US11066638
申请日：2005-02-25
CPC分类号： G03F7/70283 , G03F7/706 , G03F7/70641
摘要： A system for analyzing images of a blazed phase grating sample includes an interface configured to receive images of sample points of a blazed phase grating sample obtained by an inspection system, a memory for storing the images, and a processor. Each image is named according to a sequential naming protocol that associates each image to a location on the blazed phase grating sample. The processor is configured to load the images from the memory, convert image data for each sample point to intensity values by pixel, determine a best focus by azimuth for each sample point based on the intensity values, and calculate parameters from the blazed phase grating sample based on the best focus by azimuth for each sample point.
摘要翻译： 用于分析闪耀相位光栅样本的图像的系统包括被配置为接收由检查系统获得的闪耀相位光栅样本的采样点的图像，用于存储图像的存储器和处理器的接口的接口。 每个图像根据顺序命名协议命名，将每个图像与闪耀的相位光栅样本上的一个位置相关联。 处理器被配置为从存储器加载图像，将每个采样点的图像数据逐个像素转换为强度值，基于强度值确定每个采样点的方位角的最佳焦点，并从闪耀的相位光栅样本中计算参数 基于每个采样点的方位角的最佳焦点。
-
-
-
-
-
-
-
-
-
搜索结果
28 条记录 (耗时 0.018 秒)