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1.发明授权Method for the production of &rgr;-Hydroxybenzoate in species of pseudomonas and agrobacterium 失效在假单胞菌和农杆菌物种中生产对羟基苯甲酸酯的方法公开(公告)号：US06586229B1
公开(公告)日：2003-07-01
申请号：US09585174
申请日：2000-06-01
申请人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan Ramos , Sima Sariaslani
发明人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan Ramos , Sima Sariaslani
IPC分类号： C12N120
摘要： Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.
摘要翻译： 用pcu基因转化的细菌菌株可用于生产对羟基苯甲酸酯（PHBA）。 申请人提供了使用来自假单胞菌KR-1的对甲酚（pcu）和tmoX基因序列，由这些序列编码的蛋白质，含有此类序列的重组质粒和含有此类质粒或整合序列的细菌宿主细胞。 还公开了使用这些材料制备PHBA的方法。
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公开(公告)号：US07985849B2
公开(公告)日：2011-07-26
申请号：US12051255
申请日：2008-03-19
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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公开(公告)号：US08569469B2
公开(公告)日：2013-10-29
申请号：US12857130
申请日：2010-08-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
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公开(公告)号：US08445659B2
公开(公告)日：2013-05-21
申请号：US12857120
申请日：2010-08-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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5.发明申请REDUCTION OF 2,3-DIHYDROXY-2-METHYL BUTYRATE (DHMB) IN BUTANOL PRODUCTION 审中-公开在丁醇生产中减少2,3-二羟基-2-甲基丁酸（DHMB）公开(公告)号：US20120258873A1
公开(公告)日：2012-10-11
申请号：US13153866
申请日：2011-06-06
申请人： KATHARINE J. GIBSON , Arthur Leo Kruckeberg , Lori Ann Maggio-Hall , Mark J. Nelson , Ranjan Patnaik
发明人： KATHARINE J. GIBSON , Arthur Leo Kruckeberg , Lori Ann Maggio-Hall , Mark J. Nelson , Ranjan Patnaik
CPC分类号： C12Q1/025 , C12N9/0006 , C12P7/16 , C12Q1/26 , Y02E50/10
摘要： The invention relates generally to the field of industrial microbiology and butanol production. More specifically, the invention relates methods of reducing 2,3-dihydroxy-2-methyl butyrate (DHMB) in butanol production. DHMB can be reduced by inhibiting the reduction of acetolactate to DHMB, for example, by knocking out enzymes that catalyze the reduction or by removing DHMB during or after fermentation. Yeast strains, compositions, and methods for reducing DHMB and increasing butanol yield are provided.
摘要翻译： 本发明一般涉及工业微生物学和丁醇生产领域。 更具体地，本发明涉及在丁醇生产中还原2,3-二羟基-2-甲基丁酸酯（DHMB）的方法。 通过抑制乙酰乳酸转化为DHMB可以减少DHMB，例如通过敲除催化还原的酶或在发酵过程中或之后除去DHMB。 提供了用于降低DHMB和提高丁醇产率的酵母菌株，组合物和方法。
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公开(公告)号：US20110021767A1
公开(公告)日：2011-01-27
申请号：US12857120
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
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公开(公告)号：US07410754B1
公开(公告)日：2008-08-12
申请号：US10738052
申请日：2003-12-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B 12 - 12脱水脱水酶的序列。 使用这些12-14位脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀失活的速率。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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公开(公告)号：US20110021768A1
公开(公告)日：2011-01-27
申请号：US12857130
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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9.发明授权Method for the production of p-hydroxybenzoate in species of pseudomonas and agrobacterium 失效在假单胞菌和农杆菌物种中生产对羟基苯甲酸酯的方法公开(公告)号：US07195898B2
公开(公告)日：2007-03-27
申请号：US11484129
申请日：2006-07-11
申请人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan Ramos , Sima Sariaslani
发明人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan Ramos , Sima Sariaslani
摘要： Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.
摘要翻译： 用pcu基因转化的细菌菌株可用于生产对羟基苯甲酸酯（PHBA）。 申请人提供了使用来自假单胞菌KR-1的对甲酚（pcu）和tmoX基因序列，由这些序列编码的蛋白质，含有此类序列的重组质粒和含有此类质粒或整合序列的细菌宿主细胞。 还公开了使用这些材料制备PHBA的方法。
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10.发明授权Method for the production of p-Hydrozybenzoate in species of Pseudomonas and Agrobacterium 失效在假单胞菌和农杆菌物种中生产对羟基苯甲酸酯的方法公开(公告)号：US07083953B2
公开(公告)日：2006-08-01
申请号：US10464952
申请日：2003-06-19
申请人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan L. Ramos , Sima Sariaslani
发明人： Arie Ben-Bassat , Monica Cattermole , Anthony A. Gatenby , Katharine J. Gibson , M. Isabel Ramos-Gonzalez , Juan L. Ramos , Sima Sariaslani
摘要： Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.
摘要翻译： 用pcu基因转化的细菌菌株可用于生产对羟基苯甲酸酯（PHBA）。 申请人提供了使用来自假单胞菌KR-1的对甲酚（pcu）和tmoX基因序列，由这些序列编码的蛋白质，含有此类序列的重组质粒和含有此类质粒或整合序列的细菌宿主细胞。 还公开了使用这些材料制备PHBA的方法。
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