公开(公告)号：US07229798B2

公开(公告)日：2007-06-12

申请号：US10332834

申请日：2001-07-05

Applicant: Paul R. August ,  Pamela J. Keagle ,  Henry Long ,  Anna Wiencis Montmayeur ,  Katherine Call ,  Michael Draper

Inventor： Paul R. August ,  Pamela J. Keagle ,  Henry Long ,  Anna Wiencis Montmayeur ,  Katherine Call ,  Michael Draper

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12Q2525/155 ,  C12Q2537/143 ,  C12Q2531/113

Abstract: The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

Abstract translation: 本发明涉及插入到载体中的DNA片段的转座子介导的多重测序的自动化方法。 它更具体地涉及这种自动化方法中提高的效率，其中通过在将转座子插入到载体序列中的那些构建体进行测序之前筛选来获得提高的效率。 这样可以避免在进行反应测序时反而对感兴趣的DNA片段进行测序而浪费时间和资源。

公开(公告)号：US20060030541A1

公开(公告)日：2006-02-09

申请号：US11245147

申请日：2005-10-07

Applicant: Teresa Garcia ,  Sergio Roman Roman ,  Roland Baron ,  Katherine Call ,  Joachim Theilhaber ,  Timothy Connolly ,  Amanda Jackson ,  Steven Bushnell ,  Georges Rawadi

Inventor： Teresa Garcia ,  Sergio Roman Roman ,  Roland Baron ,  Katherine Call ,  Joachim Theilhaber ,  Timothy Connolly ,  Amanda Jackson ,  Steven Bushnell ,  Georges Rawadi

IPC: A61K48/00 ,  C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6883 ,  C07K14/47 ,  C12Q2600/136 ,  C12Q2600/158

Abstract: The present invention relates to methods of diagnosis, therapy, and screening of new therapeutic compounds in the field of osteogenesis, based on the differential expression observed for the genes of the invention, represented by SEQ ID No 2, 7, 22, 25, 33, 35, 37, 38, 42, 47, 64, 69 or 77, 82, 97, 100, 108, 110, 112, 113, 117, 122, 139, 151, 158, 160, 172, 182, 184, 187, 188, 194.

Abstract translation: 本发明涉及基于由SEQ ID No.2,7,22,25,33所示的本发明基因观察到的差异表达，在成骨领域中新治疗化合物的诊断，治疗和筛选方法 ，35,37,38,42,47,64,69或77,82,97,100,108,110,112,113,117,122,139,151,158,160,172,182,184,187 ，188,194。