公开(公告)号：US10752913B2

公开(公告)日：2020-08-25

申请号：US12517906

申请日：2007-12-07

Applicant: Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven R. Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

Inventor： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven R. Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

IPC: C12N15/82

Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

公开(公告)号：US07888069B2

公开(公告)日：2011-02-15

申请号：US11962924

申请日：2007-12-21

Applicant: Kelley A. Smith ,  Steven R. Webb ,  Steven L. Evans ,  Charles A. Mihaliak ,  Donald J. Merlo ,  Geoffrey J. Letchworth

Inventor： Kelley A. Smith ,  Steven R. Webb ,  Steven L. Evans ,  Charles A. Mihaliak ,  Donald J. Merlo ,  Geoffrey J. Letchworth

IPC: C12N15/40 ,  C12N15/62 ,  C12N5/10 ,  A01H5/00

CPC classification number: C12N15/8258 ,  A61K39/12 ,  A61K2039/55555 ,  A61K2039/55566 ,  C07K14/005 ,  C12N2770/24122 ,  C12N2770/24134 ,  Y02A50/394

Abstract: The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.

Abstract translation: 本申请提供针对西尼罗河病毒（WNV）多肽及其片段的物质的多种组合物，以及如本文所述编码，指导表达或产生WNV多肽的多核苷酸，载体和转化的宿主细胞。 本文还提供了使用多肽和多核苷酸在个体中产生免疫应答或检测WNV特异性或中和抗体的存在的方法。

公开(公告)号：US07179902B2

公开(公告)日：2007-02-20

申请号：US10603524

申请日：2003-06-25

Applicant: Neil M. Cowen ,  Katherine Armstrong ,  Kelley A. Smith

Inventor： Neil M. Cowen ,  Katherine Armstrong ,  Kelley A. Smith

IPC: A01H1/00 ,  C07H21/04 ,  C12N5/14 ,  C12N15/09

CPC classification number: C12N15/8216

Abstract: The present invention provides DNA sequences that function as 3′ untranslated regions (3′UTR) in plants. The 3′UTR's stabilize associated recombinant transcripts such that expression is improved. The invention further provides plant expression cassettes and recombinant plant that comprise a claimed 3′UTR.

Abstract translation: 本发明提供在植物中作为3'非翻译区（3'UTR）起作用的DNA序列。 3'UTR稳定相关的重组转录物，使得表达得到改善。 本发明还提供了包含所要求保护的3'UTR的植物表达盒和重组植物。

公开(公告)号：US08632978B2

公开(公告)日：2014-01-21

申请号：US13558982

申请日：2012-07-26

Applicant: Lauren Clark ,  Kelley A. Smith ,  Yang Wang ,  Ning Zhou

Inventor： Lauren Clark ,  Kelley A. Smith ,  Yang Wang ,  Ning Zhou

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6895 ,  C12Q2600/16

Abstract: Soybean Event pDAB9582.814.19.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The invention provides PCR event detection methods.

Abstract translation: 大豆事件pDAB9582.814.19.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。 本发明提供PCR事件检测方法。

公开(公告)号：US20110107455A1

公开(公告)日：2011-05-05

申请号：US12517906

申请日：2007-12-07

Applicant: Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

Inventor： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

IPC: C12N15/82 ,  C12N5/10 ,  C12N15/63 ,  C12Q1/04 ,  A01H5/10 ,  A01H1/00 ,  C12N9/10 ,  C12N1/21 ,  A01N57/18 ,  C12Q1/68 ,  A01H5/00

Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Abstract translation: 本发明涉及本文称为DSM-2的新基因。 该基因在天竺葵A3中鉴定。 DSM-2蛋白与PAT和BAR密切相关。 本发明还提供编码DSM-2蛋白质的植物优化基因，DSM-2可用作转基因性状，以将植物和植物细胞中的耐受性赋予除草剂草铵膦和双丙二酸。 主题基因的一个优选用途是选择标记。 在细菌系统中使用该基因作为选择标记可以提高植物转化的效率。 使用DSM-2作为唯一选择标记，在克隆过程中不需要额外的药用抗生素标记（如氨苄青霉素抗性）。 根据本发明，各种其他用途也是可能的。

公开(公告)号：US07071385B2

公开(公告)日：2006-07-04

申请号：US10378810

申请日：2003-03-04

Applicant: Beth C. Rubin-Wilson ,  Kelley A. Smith

Inventor： Beth C. Rubin-Wilson ,  Kelley A. Smith

IPC: A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/11

CPC classification number: C12N15/8216 ,  C12N15/8209

Abstract: The promoter from the maize actin depolymerizing factor gene is useful in controlling transgene expression in plants.

Abstract translation: 来自玉米肌动蛋白解聚因子基因的启动子可用于控制植物中的转基因表达。

公开(公告)号：US20100261884A1

公开(公告)日：2010-10-14

申请号：US12821815

申请日：2010-06-23

Applicant: William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

Inventor： William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

IPC: C07K14/33

CPC classification number: A61K39/08 ,  A61K2039/552 ,  A61K2039/55577 ,  C07K14/33

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract translation: 本发明部分涉及针对坏死性肠炎（NE）的家禽疫苗的开发。 该疫苗使用作为突变的全长无毒产气荚膜梭菌（Cp）α-毒素蛋白（Mcpa）的保护性抗原。 例如，通过减少NE攻击路径中的病变严重程度来证明该疫苗的效用。 本文还公开了用于大量生产该疫苗的新方法。 一个例举的方法涉及在细菌表达系统中产生NE疫苗（突变的α毒素），优选利用荧光假单胞菌系统，用于商业用于控制家禽业中的NE。 本发明的疫苗可以以几种不同的方式优选施用于鸡。 还公开了来自Cp的鸡分离株的新型VI型α毒素。

公开(公告)号：US07745175B2

公开(公告)日：2010-06-29

申请号：US11925300

申请日：2007-10-26

Applicant: William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

Inventor： William M. Ainley ,  Janna Armstrong ,  Krishna Madduri ,  Donald J. Merlo ,  Kelley A. Smith ,  Mark A. Thompson ,  Steven R. Webb ,  Liu Y. Shen

IPC: A61K8/00 ,  C07H21/04

CPC classification number: A61K39/08 ,  A61K2039/552 ,  A61K2039/55577 ,  C07K14/33

Abstract: This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

Abstract translation: 本发明部分涉及针对坏死性肠炎（NE）的家禽疫苗的开发。 该疫苗使用作为突变的全长无毒产气荚膜梭菌（Cp）α-毒素蛋白（Mcpa）的保护性抗原。 例如，通过减少NE攻击路径中的病变严重程度来证明该疫苗的效用。 本文还公开了用于大量生产该疫苗的新方法。 一个例举的方法涉及在细菌表达系统中产生NE疫苗（突变的α毒素），优选利用荧光假单胞菌系统，用于商业用于控制家禽业中的NE。 本发明的疫苗可以以几种不同的方式优选施用于鸡。 还公开了来自Cp的鸡分离株的新型VI型α毒素。

公开(公告)号：US20130065230A1

公开(公告)日：2013-03-14

申请号：US13558982

申请日：2012-07-26

Applicant: Lauren CLARK ,  Kelley A. SMITH ,  Yang WANG ,  Ning ZHOU

Inventor： Lauren CLARK ,  Kelley A. SMITH ,  Yang WANG ,  Ning ZHOU

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/16

Abstract: Soybean Event pDAB9582.814.19.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The invention provides PCR event detection methods.

Abstract translation: 大豆事件pDAB9582.814.19.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。 本发明提供PCR事件检测方法。

公开(公告)号：US06791013B2

公开(公告)日：2004-09-14

申请号：US09727628

申请日：2000-12-01

Applicant: Katherine Armstrong ,  Timothy D. Hey ,  Otto Folkerts ,  Kelley A. Smith ,  Nicole L. Hopkins

Inventor： Katherine Armstrong ,  Timothy D. Hey ,  Otto Folkerts ,  Kelley A. Smith ,  Nicole L. Hopkins

IPC: A01H500

CPC classification number: C12N15/8222 ,  C12N9/90 ,  C12N15/8234

Abstract: The maize myo-inositol-1-phosphate synthase (MIP synthase) promoter is a novel embryo specific regulatory sequence useful in expressing heterologous nucleic acids in plants.

Abstract translation: 玉米肌醇-1-磷酸合成酶（MIP合酶）启动子是用于在植物中表达异源核酸的新型胚胎特异性调节序列。