公开(公告)号：US08632714B2

公开(公告)日：2014-01-21

申请号：US13526708

申请日：2012-06-19

申请人： Masahiko Ogino ,  Akihiro Miyauchi ,  Sigehisa Motowaki ,  Kosuke Kuwabara

发明人： Masahiko Ogino ,  Akihiro Miyauchi ,  Sigehisa Motowaki ,  Kosuke Kuwabara

IPC分类号： B28B11/08 ,  B27N3/18 ,  A01J21/00

CPC分类号： B81C1/0046

摘要： A mold and a pattern transfer method using the same for a nanoprinting technology. The mold can be released from a substrate accurately and easily. The mold, which is used for forming a fine pattern on a substrate using a press machine, comprises a release mechanism.

摘要翻译： 用于纳米印刷技术的模具和图案转印方法。 模具可以准确而容易地从基材上脱模。 使用压制机在基板上形成精细图案的模具包括释放机构。

公开(公告)号：US20120069333A1

公开(公告)日：2012-03-22

申请号：US13303830

申请日：2011-11-23

申请人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

发明人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC分类号： G01J3/28

CPC分类号： G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

摘要： The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

摘要翻译： 本发明能够使用能够填充少量化学溶液的小细胞来测量生物化学物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测靶与探针的结合。

公开(公告)号：US07834998B2

公开(公告)日：2010-11-16

申请号：US12662928

申请日：2010-05-12

申请人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

发明人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC分类号： G01N21/00 ,  G01N21/01

CPC分类号： G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

摘要： The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

摘要翻译： 本发明能够使用能够填充少量化学溶液的小细胞来测量生化物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测靶与探针的结合。

公开(公告)号：US07742165B2

公开(公告)日：2010-06-22

申请号：US11699362

申请日：2007-01-30

申请人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

发明人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC分类号： G01N21/00 ,  G01N21/01

CPC分类号： G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

摘要： The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the reflection spectrum.

摘要翻译： 本发明能够使用能够填充少量化学溶液的小细胞来测量生化物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测靶与探针的结合。

公开(公告)号：US07520742B2

公开(公告)日：2009-04-21

申请号：US10801579

申请日：2004-03-17

申请人： Shigehisa Motowaki ,  Akihiro Miyauchi ,  Masahiko Ogino ,  Kosuke Kuwabara

发明人： Shigehisa Motowaki ,  Akihiro Miyauchi ,  Masahiko Ogino ,  Kosuke Kuwabara

IPC分类号： B29C33/00 ,  B29C59/02

CPC分类号： B82Y10/00 ,  B29C33/58 ,  B29C37/0067 ,  B29C37/0075 ,  B29C59/022 ,  B29C59/025 ,  B29C2059/023 ,  B82Y40/00 ,  G03F7/0002 ,  G11B5/74 ,  G11B5/743 ,  G11B5/82 ,  G11B5/84 ,  H05K3/12 ,  Y10S977/887

摘要： A pillar with a high aspect ratio is transferred by a nanoprinting method. In order to form a fine structure on a substrate, a nanoprinting apparatus heats and presses the substrate and a mold with a fine concave-convex pattern formed thereon, the mold having a mechanism for transferring and applying a mold-releasing agent. A method for transferring a fine structure using the aforementioned nanoprinting apparatus.

摘要翻译： 通过纳米印刷法转移具有高纵横比的支柱。 为了在基板上形成精细结构，纳米印刷装置加热并压制基板和形成有细凹凸图案的模具，该模具具有用于转印和施加脱模剂的机构。 使用上述纳米印刷装置转印精细结构的方法。

公开(公告)号：US20080229948A1

公开(公告)日：2008-09-25

申请号：US12019839

申请日：2008-01-25

申请人： Ryuta Washiya ,  Takashi Ando ,  Masahiko Ogino ,  Hideaki Kataho ,  Akihiro Miyauchi ,  Kosuke Kuwabara

发明人： Ryuta Washiya ,  Takashi Ando ,  Masahiko Ogino ,  Hideaki Kataho ,  Akihiro Miyauchi ,  Kosuke Kuwabara

IPC分类号： B41F1/40

CPC分类号： G03F7/0002 ,  B82Y10/00 ,  B82Y40/00

摘要： An imprint device transfers a micropattern created on a stamper onto a material to be transferred, by bringing the stamper and the material to be transferred into contact with each other. The imprint device has a flow passage for discharging a fluid to a rear surface of the stamper or the material to be transferred, to thereby bend the stamper or the material to be transferred.

摘要翻译： 压印装置通过使压模和被转印材料彼此接触来将形成在压模上的微图案转印到待转印的材料上。 压印装置具有用于将流体排放到压模的后表面或待转印的材料的流动通道，从而弯曲压模或要转印的材料。

公开(公告)号：US20070212259A1

公开(公告)日：2007-09-13

申请号：US11699362

申请日：2007-01-30

申请人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

发明人： Toru Fujimura ,  Kosuke Kuwabara ,  Tatsuro Ide

IPC分类号： G01N21/00

CPC分类号： G01N21/05 ,  G01N21/31 ,  G01N21/55 ,  G01N33/54373 ,  G01N2021/0346 ,  G01N2201/061 ,  G01N2201/08

摘要： The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

摘要翻译： 本发明能够使用能够填充少量化学溶液的小细胞来测量生物化学物质与高产量和高灵敏度的结合。 使用第一基板和第二基板之间的空间，使得将探针固定在其相互面对的平面上，作为容纳样本溶液的单元。 从第一基板侧照射光，反射光进行光谱分析。 通过反射光谱中的波长偏移来检测靶与探针的结合。

公开(公告)号：US20070190786A1

公开(公告)日：2007-08-16

申请号：US11623817

申请日：2007-01-17

申请人： Kenji Murao ,  Kosuke Kuwabara ,  Masahiko Ogino

发明人： Kenji Murao ,  Kosuke Kuwabara ,  Masahiko Ogino

IPC分类号： H01L21/302 ,  H01L21/461

CPC分类号： B81C1/0046 ,  B29C43/021 ,  B29C2043/025 ,  B81B2201/0214 ,  B81B2203/0361 ,  B81C2201/0153 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y40/00 ,  G03F7/0002

摘要： It is an object of the present invention to provide a method which can easily and selectively modify specific sites on indentations or protrusions of indentation/protrusion structures fabricated by nano-imprinting. Pressing a mold having indentation/protrusion structures onto a polymer substrate comprising at least two layers of different chemical composition exposes the second layer, which has been covered by the outermost layer, in pillars formed as a result of the pressing. Site-specific chemical modification of the pillars can be achieved by formulating a desired chemical composition for the second layer beforehand, or by chemical modification of the exposed second layer cross-sections in the pillars.

摘要翻译： 本发明的一个目的是提供一种方法，其可以容易且选择性地改变通过纳米压印制造的压痕/突起结构的凹陷或凸起上的特定部位。 将具有凹陷/突起结构的模具压到包含至少两层不同化学组成的聚合物基材上，将由最外层覆盖的第二层暴露在由压制形成的柱中。 可以通过预先为第二层配制所需的化学组合物，或者通过化学修饰柱中暴露的第二层横截面来实现柱的特定位点的化学修饰。

公开(公告)号：US20050214935A1

公开(公告)日：2005-09-29

申请号：US10989910

申请日：2004-11-17

申请人： Kosuke Kuwabara ,  Akihiro Miyauchi ,  Masahiko Ogino ,  Takashi Ando

发明人： Kosuke Kuwabara ,  Akihiro Miyauchi ,  Masahiko Ogino ,  Takashi Ando

IPC分类号： C12M1/14 ,  C12M3/00 ,  C12M3/04

CPC分类号： C12M23/20 ,  C12M21/08 ,  C12M23/10

摘要： It is an object of this invention to provide a cell culture vessel which has a simple and convenient structure, can prevent the injury on the cells at the time of peeling, and accelerates the transportation of nutrients and discharge of waste materials. In order to solve the problem mentioned above, the cell culture vessel of this invention has protrusions having a corresponding diameter not smaller than 10 nm and not greater than 10 μm and a height not smaller than 10 nm and not greater than 1 mm on the surface of the cell culture vessel. The protrusions make the culture fluid permeate into the under part of the cells, accelerate the supply of nutrients and discharge of waste materials, and makes the contact between the cells and the vessel as a point-contact and thereby prevents the cells from the injury which the cells undergo at the time of peeling.

摘要翻译： 本发明的目的是提供一种结构简单方便的细胞培养容器，可以防止剥离时对细胞的损伤，加速营养物质的运输和废料排出。 为了解决上述问题，本发明的细胞培养容器具有相对应的直径不小于10nm且不大于10um的突起，并且在表面上具有不小于10nm且不大于1mm的高度 的细胞培养容器。 突起使得培养液渗透到细胞的下部，加速营养物质的供给和排出废物，并使细胞与血管之间的接触作为点接触，从而防止细胞受损， 细胞在剥离时经历。

公开(公告)号：US08132505B2

公开(公告)日：2012-03-13

申请号：US11774216

申请日：2007-07-06

申请人： Kosuke Kuwabara ,  Takashi Ando ,  Masahiko Ogino ,  Hiroshi Yoshida ,  Yasuhiko Tada

发明人： Kosuke Kuwabara ,  Takashi Ando ,  Masahiko Ogino ,  Hiroshi Yoshida ,  Yasuhiko Tada

IPC分类号： B31F1/07

CPC分类号： G03F7/0002 ,  B82Y10/00 ,  B82Y40/00 ,  G11B5/855 ,  H01L21/76817

摘要： The present invention provides an imprint apparatus transferring a surface structure of a stamper to material to be patterned by allowing the stamper come in contact with the material to be patterned. The imprint apparatus includes a holding means that holds the material to be patterned and the stamper with a distance therebetween, a pressure reduction means that reduces pressure of a chamber in which the material to be patterned and the stamper are placed, and an alignment means that aligns the stamper with the material to be patterned.

摘要翻译： 本发明提供一种压印装置，通过允许压模与待图案化的材料接触，将压模的表面结构转移到待图案化的材料。 压印装置包括保持装置，其保持要被图案化的材料和压模之间的距离;减压装置，其减小其中待图案化材料和压模放置的室的压力;以及对准装置， 将压模与待图案化的材料对准。