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    Method for producing human epidermal growth factor in large volume from yeast
    4.
    发明授权
    Method for producing human epidermal growth factor in large volume from yeast 有权
    从酵母中大量生产人表皮生长因子的方法

    公开(公告)号：US08986956B2

    公开(公告)日：2015-03-24

    申请号：US13883264

    申请日：2011-11-04

    申请人： Jung Hoon Sohn ,  Jung Hoon Bae ,  Mi Jin Kim ,  Hyun Jin Kim ,  Soon Ho Park ,  Kwang Mook Lim

    发明人： Jung Hoon Sohn ,  Jung Hoon Bae ,  Mi Jin Kim ,  Hyun Jin Kim ,  Soon Ho Park ,  Kwang Mook Lim

    IPC分类号： C12P21/04 ,  C12N1/16 ,  C12N15/09 ,  C07H21/04 ,  C07K14/485 ,  C12N15/81 ,  C12R1/645

    CPC分类号： C07K14/485 ,  C12N15/81 ,  C12R1/645

    摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.

    摘要翻译： 本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。 更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。 使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。

    METHOD FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR IN LARGE VOLUME FROM YEAST
    5.
    发明申请
    METHOD FOR PRODUCING HUMAN EPIDERMAL GROWTH FACTOR IN LARGE VOLUME FROM YEAST 有权
    从日本大批量生产人类生殖生长因子的方法

    公开(公告)号：US20130323785A1

    公开(公告)日：2013-12-05

    申请号：US13883264

    申请日：2011-11-04

    申请人： Jung Hoon Sohn ,  Jung Hoon Bae ,  Mi Jin Kim ,  Hyun Jin Kim ,  Soon Ho Park ,  Kwang Mook Lim

    发明人： Jung Hoon Sohn ,  Jung Hoon Bae ,  Mi Jin Kim ,  Hyun Jin Kim ,  Soon Ho Park ,  Kwang Mook Lim

    IPC分类号： C07K14/485 ,  C12R1/645

    CPC分类号： C07K14/485 ,  C12N15/81 ,  C12R1/645

    摘要： The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.

    摘要翻译： 本发明涉及以高浓度和高纯度生产具有与野生型相同的活性的hEGF（人表皮生长因子）的方法。 更具体地，本发明涉及包含编码序列号14的多肽的核酸序列的hEGF表达载体; 其中表达载体已被遗传转化的宿主细胞; 以及生产hEGF的方法，包括其中缺少表达载体并在KEX1基因缺失的酵母中遗传转化的步骤。 使用本发明的方法，可以生产与人源性EGF具有相同大小和活性的大量人源性EGF，并且该EGF可以以各种方式用于医药和化妆品中。