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1.发明申请Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use 有权羧酸还原酶多肽，编码核苷酸序列和使用方法公开(公告)号：US20060199254A1
公开(公告)日：2006-09-07
申请号：US11431321
申请日：2006-05-10
申请人： John Rosazza , Ian Fotheringham , Tao Li , Lacy Daniels , Aimin He
发明人： John Rosazza , Ian Fotheringham , Tao Li , Lacy Daniels , Aimin He
IPC分类号： C12P7/24 , C12N9/02 , C07C49/786
CPC分类号： C12N9/0008 , C07H21/04
摘要： The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.
摘要翻译： 本发明提供了从细菌分离的酶羧酸还原酶的核苷酸序列和氨基酸序列。 还提供了表达盒，载体，转化细胞和变体作为在合成，芳族，脂族和脂环族醛和醇的生产中使用重组生物催化剂的方法。
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2.发明授权公开(公告)号：US07056714B2
公开(公告)日：2006-06-06
申请号：US10386329
申请日：2003-03-11
申请人： John P. Rosazza , Ian Fotheringham , Tao Li , Lacy Daniels , Aimin He
发明人： John P. Rosazza , Ian Fotheringham , Tao Li , Lacy Daniels , Aimin He
CPC分类号： C12N9/0008 , C07H21/04
摘要： The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.
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3.发明授权Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use 有权羧酸还原酶多肽，编码核苷酸序列和使用方法公开(公告)号：US07425433B2
公开(公告)日：2008-09-16
申请号：US11431321
申请日：2006-05-10
申请人： John P. Rosazza , Ian Fotherington , Tao Li , Lacy Daniels , Aimin He
发明人： John P. Rosazza , Ian Fotherington , Tao Li , Lacy Daniels , Aimin He
CPC分类号： C12N9/0008 , C07H21/04
摘要： The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols.
摘要翻译： 本发明提供了从细菌分离的酶羧酸还原酶的核苷酸序列和氨基酸序列。 还提供了表达盒，载体，转化细胞和变体作为在合成，芳族，脂族和脂环族醛和醇的生产中使用重组生物催化剂的方法。
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公开(公告)号：US4933281A
公开(公告)日：1990-06-12
申请号：US26857
申请日：1987-03-17
CPC分类号： C07H13/04 , C07C59/01 , C12P19/44 , Y10S435/822 , Y10S435/874 , Y10S435/875
摘要： A method for large-scale production of rhamnose and 3-hydroxydecanoic acid is disclosed comprising the steps of growing microorganisms of Pseudomonas sp. capable of production of high levels of rhamnolipid in a defined culture medium containing vegetable oil. Additional steps include isolating the rhamnolipid from the culture medium, hydrolyzing the rhamnolipid to produce rhamnose and 3-hydroxydecanoic acid, and separating the rhamnose from the acid. Corn oil is the preferred vegetable oil and Pseudomonas aeruginosa is the preferred Pseudomonas sp. Non-limiting concentrations of nitrogen compounds and magnesium compounds and limiting concentrations of iron compounds are additionally preferably included in the culture medium.
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