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公开(公告)号:US20160215254A1
公开(公告)日:2016-07-28
申请号:US14909195
申请日:2014-09-23
申请人: Deirdre MELDRUM , Shih-Hui (Joseph) CHAO , Thai TRAN , Laimonas BAUSKAS , Jeff HOUKAL , Andrew HATCH , Weimin GAO , David Wayne RICHARDSON
发明人: Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
CPC分类号: C12Q1/6806 , B01L3/0293 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M47/06 , C12Q1/6841 , C12Q1/6848 , C12Q1/686 , G01N1/286 , G01N2001/2886 , C12Q2547/101
摘要: The present invention provides a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing the at least one target molecule.
摘要翻译: 本发明提供了一种用于原位裂解个体细胞的系统和方法,包括以下步骤:捕获包含细胞内容物的组织样品,使组织样品经受连续流体流动的流动,将组织样品的选定区域用 激光,从而从组织样品中释放至少一部分细胞含量,从流中的细胞含量回收至少一种靶分子,以及处理该至少一种靶分子。