公开(公告)号：US20160215254A1

公开(公告)日：2016-07-28

申请号：US14909195

申请日：2014-09-23

申请人： Deirdre MELDRUM ,  Shih-Hui (Joseph) CHAO ,  Thai TRAN ,  Laimonas BAUSKAS ,  Jeff HOUKAL ,  Andrew HATCH ,  Weimin GAO ,  David Wayne RICHARDSON

发明人： Deirdre Meldrum ,  Shih-Hui (Joseph) Chao ,  Thai Tran ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Andrew Hatch ,  Weimin Gao ,  David Richardson

IPC分类号： C12M1/00 ,  G01N1/28 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  B01L3/0293 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M47/06 ,  C12Q1/6841 ,  C12Q1/6848 ,  C12Q1/686 ,  G01N1/286 ,  G01N2001/2886 ,  C12Q2547/101

摘要： The present invention provides a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing the at least one target molecule.

摘要翻译： 本发明提供了一种用于原位裂解个体细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流动的流动，将组织样品的选定区域用 激光，从而从组织样品中释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，以及处理该至少一种靶分子。

公开(公告)号：US09597026B2

公开(公告)日：2017-03-21

申请号：US14538557

申请日：2014-11-11

申请人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

发明人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

IPC分类号： A61B5/1455 ,  A61B5/1459 ,  A61B5/00

CPC分类号： A61B5/1459 ,  A61B5/1455 ,  A61B5/6847

摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.

公开(公告)号：US20150133757A1

公开(公告)日：2015-05-14

申请号：US14538557

申请日：2014-11-11

申请人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

发明人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Roger Johnson ,  Yanqing Tian ,  Cody Youngbull

IPC分类号： A61B5/1459 ,  A61B1/07

CPC分类号： A61B5/1459 ,  A61B5/1455 ,  A61B5/6847

摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.

摘要翻译： 包括使用光纤技术的改进的内窥镜和多个外在光学传感器的新型方法和装置，用于实现同时测定生物分析物在体内的单细胞分辨率的变化率。 这些装置采用动态振荡致动来确定相对于电池的一个或多个方向的感兴趣的分析物的梯度。 感兴趣的分析物的变化率或通量可以很容易地通过应用与流量和浓度梯度相关的第一个菲克定律来确定。 器件的双重工作模式显着提高了测量灵敏度和精度。

公开(公告)号：US11045807B2

公开(公告)日：2021-06-29

申请号：US16479729

申请日：2018-02-26

申请人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Yanqing Tian ,  Honor Glenn ,  Clifford Anderson ,  Kristen Lee ,  Ganquan Song ,  Liqiang Zhang ,  Jeff Houkal ,  Fengyu Su ,  Benjamin Ueberroth ,  Jacob Messner ,  Hong Wang ,  Kimberly Bussey

发明人： Deirdre Meldrum ,  Laimonas Kelbauskas ,  Yanqing Tian ,  Honor Glenn ,  Clifford Anderson ,  Kristen Lee ,  Ganquan Song ,  Liqiang Zhang ,  Jeff Houkal ,  Fengyu Su ,  Benjamin Ueberroth ,  Jacob Messner ,  Hong Wang ,  Kimberly Bussey

IPC分类号： B01L3/00 ,  G01N15/14 ,  G02B21/34 ,  G01N15/10

摘要： An integrated technological platform enabling real-time quantitative multiparameter metabolic profiling, utilizing either or both of extra and intracellular optical sensors, individually or simultaneously. A scalable embedded micropocket array structure, generally fabricated on fused silica substrates, facilitates the integration of multiple, spatially separated extracellular sensors for multiparameter analysis in a container formed with the use of an activation mechanism forming part of a device configured to hold the container during the measurements. The creation of hermetically sealed microchambers is carried out with a pneumatically and/or mechanically and/or electromechanically driven device that is “floating” within the holding device and that is optionally equipped with a vacuum/suction mechanism to hold a component of the container at its surface.

公开(公告)号：US20150126404A1

公开(公告)日：2015-05-07

申请号：US14533349

申请日：2014-11-05

申请人： Yanqing Tian ,  Liqiang Zhang ,  Fengyu Su ,  Deirdre Meldrum ,  Sean Buizer ,  Clifford L. Anderson ,  Laimonas Kelbauskas ,  Kristen Lee

发明人： Yanqing Tian ,  Liqiang Zhang ,  Fengyu Su ,  Deirdre Meldrum ,  Sean Buizer ,  Clifford L. Anderson ,  Laimonas Kelbauskas ,  Kristen Lee

IPC分类号： G01N33/50 ,  G01N21/64 ,  G01N33/84

CPC分类号： G01N33/84 ,  G01N21/6428 ,  G01N33/66

摘要： A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.

摘要翻译： 三重传感器，用于同时测量葡萄糖，氧气和pH值。 传感器组件处于薄膜状态，例如感测膜或膜，葡萄糖探针与光谱蓝色部分中的辐射相关，与红色光谱中的辐射相关的氧探针，以及pH探针 - 具有绿色部分的光谱。 光学探针被化学接枝或固定在合适的聚合物基质中，减轻了探针从基体中的浸出，改善了薄膜感测的稳定性，并使得能够重复使用相同的传感膜。

公开(公告)号：US20150087007A1

公开(公告)日：2015-03-26

申请号：US14497005

申请日：2014-09-25

申请人： Deirdre Meldrum ,  Roger Johnson ,  Iniyan Soundappa Elango ,  Andrew Shabilla ,  Hong Wang ,  Jakrey Myers ,  Laimonas Kelbauskas ,  Dean Smith ,  Pimwadee Limsirichai

发明人： Deirdre Meldrum ,  Roger Johnson ,  Iniyan Soundappa Elango ,  Andrew Shabilla ,  Hong Wang ,  Jakrey Myers ,  Laimonas Kelbauskas ,  Dean Smith ,  Pimwadee Limsirichai

IPC分类号： G01N33/50 ,  B01L3/00 ,  C23F1/30

CPC分类号： B01L3/502707 ,  B01L3/502761 ,  B01L2300/0645 ,  C12M23/16 ,  C12M41/36 ,  C23F1/02 ,  C23F1/30 ,  C23F1/44

摘要： A microfluidic device useable for performing live cell computed tomography imaging is fabricated with a cover portion including a first wafer with at least one metal patterned thereon, a base portion including a second wafer with at least one metal patterned thereon and negative photoresist defining recesses therein, and a diffusive bonding layer including a negative photoresist arranged to join the cover portion and the base portion. A composition useful in live cell computer topography includes a long-chain polysaccharide at a concentration of from about 0.01% to about 10.0% in cell culture medium for supporting cell life while enabling cell rotation rate to be slowed to a speed commensurate with low light level imaging.

摘要翻译： 用于执行活细胞计算机断层摄影成像的微流体装置制造成具有覆盖部分，所述覆盖部分包括具有图案化的至少一个金属的第一晶片，包括至少一个图案化的金属的第二晶片的基部和其中限定凹部的负光致抗蚀剂， 以及包括负极光致抗蚀剂的漫射粘结层，所述负性光致抗蚀剂被布置成接合盖部分和基部。 用于活细胞计算机地形的组合物包括在细胞培养基中浓度为约0.01％至约10.0％的长链多糖，用于支持细胞寿命，同时使细胞旋转速率降低至与低光级相当的速度 成像。

公开(公告)号：US20160202247A1

公开(公告)日：2016-07-14

申请号：US14996170

申请日：2016-01-14

申请人： Yanqing Tian ,  Hong Wang ,  Ganquan Song ,  Fengyu Su ,  Liqiang Zhang ,  Deirdre Meldrum ,  Laimonas Kelbauskas ,  Benjamin Ueberroth

发明人： Yanqing Tian ,  Hong Wang ,  Ganquan Song ,  Fengyu Su ,  Liqiang Zhang ,  Deirdre Meldrum ,  Laimonas Kelbauskas ,  Benjamin Ueberroth

IPC分类号： G01N33/52 ,  G01N21/64

CPC分类号： G01N33/84 ,  G01N31/221 ,  G01N31/225

摘要： The present disclosure relates to an optical luminescence dual sensor comprising a polymerized form of a probe for sensing pH; a polymerized form of a probe for sensing oxygen; a polymerized form of an internal reference probe; and a matrix. The present disclosure also relates to methods of preparing an optical luminescence dual sensor and methods of using them.

摘要翻译： 本公开涉及一种光学发光双传感器，其包括用于感测pH的探针的聚合形式; 用于感测氧的探针的聚合形式; 内部参考探针的聚合形式; 和矩阵。 本公开还涉及制备光学发光双传感器的方法及其使用方法。

公开(公告)号：US20120231533A1

公开(公告)日：2012-09-13

申请号：US13126700

申请日：2009-10-28

申请人： Mark R. Holl ,  Deirdre R. Meldrum ,  A. Cody Youngbull ,  Haixin Zhu ,  Jeff Houkal ,  Yanqing Tian ,  Shashanka Ashili ,  Laimonas Kelbauskas ,  Roger Johnson ,  Shih-hui Chao ,  Peter Wiktor ,  Alex Jen ,  Lloyd Burgess ,  Sarah McQuaid ,  Ai Brunner ,  Peter Kahn

发明人： Mark R. Holl ,  Deirdre R. Meldrum ,  A. Cody Youngbull ,  Haixin Zhu ,  Jeff Houkal ,  Yanqing Tian ,  Shashanka Ashili ,  Laimonas Kelbauskas ,  Roger Johnson ,  Shih-hui Chao ,  Peter Wiktor ,  Alex Jen ,  Lloyd Burgess ,  Sarah McQuaid ,  Ai Brunner ,  Peter Kahn

IPC分类号： C12M1/34

CPC分类号： C12M23/16 ,  C12M23/12 ,  C12M23/22 ,  C12M23/38 ,  C12M41/46

摘要： A chamber device for analyzing living cell(s). The chamber device includes a base and a lid that when reversibly pressed closed create a chamber. The base is configured with an optically transparent well to contain at least one cell. The lid has a breadth greater than the base and is configured to contain at least one sensor. The lid is further configured with a lip that when pressed between the lid and the base creates an impermeable seal. The base and the lid are configured so that, when closed and in use, the sensor remains spatially apart from the at least one cell.

摘要翻译： 用于分析活细胞的室装置。 腔室装置包括底座和盖子，当可逆地按压时，盖子产生一个腔室。 基底配置有光学透明的孔以容纳至少一个细胞。 盖子的宽度大于基座，并被配置成包含至少一个传感器。 盖子进一步配置有唇部，当在盖子和基座之间被按压时，唇部产生不可渗透的密封。 底座和盖被构造成使得当关闭并且在使用中时，传感器在空间上保持与至少一个单元隔开。