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公开(公告)号：US20060207891A1
公开(公告)日：2006-09-21
申请号：US10553584
申请日：2004-04-16
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N33/5438 , Y10T436/143333 , C12Q2565/607 , C12Q2563/173
摘要： The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reverisbly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.
摘要翻译： 本发明涉及一种用于检测靶多核苷酸的电化学方法。 提供了包括电极表面的电极。 电极表面包括相对于电极表面反复固定的至少一个探针分子。 获得指示相对于电极表面固定的探针分子的量的第一电化学信号。 电极表面与包含靶多核苷酸的液体接触。 在接触步骤中，相对于电极表面固定的至少一些探针分子与其分离。 获得指示相对于电极表面固定的探针分子的量的第二电化学信号。 至少部分地基于第一和第二电化学信号确定靶多核苷酸的存在。
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公开(公告)号：US07820030B2
公开(公告)日：2010-10-26
申请号：US10553584
申请日：2004-04-16
申请人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
发明人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N33/5438 , Y10T436/143333 , C12Q2565/607 , C12Q2563/173
摘要： The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reversibly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.
摘要翻译： 本发明涉及一种用于检测靶多核苷酸的电化学方法。 提供了包括电极表面的电极。 电极表面包括相对于电极表面可逆地固定的至少一个探针分子。 获得指示相对于电极表面固定的探针分子的量的第一电化学信号。 电极表面与包含靶多核苷酸的液体接触。 在接触步骤中，相对于电极表面固定的至少一些探针分子与其分离。 获得指示相对于电极表面固定的探针分子的量的第二电化学信号。 至少部分地基于第一和第二电化学信号确定靶多核苷酸的存在。
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公开(公告)号：US08105477B2
公开(公告)日：2012-01-31
申请号：US12910112
申请日：2010-10-22
申请人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
发明人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N33/5438 , Y10T436/143333 , C12Q2565/607 , C12Q2563/173
摘要： The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reversibly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.
摘要翻译： 本发明涉及一种用于检测靶多核苷酸的电化学方法。 提供了包括电极表面的电极。 电极表面包括相对于电极表面可逆地固定的至少一个探针分子。 获得指示相对于电极表面固定的探针分子的量的第一电化学信号。 电极表面与包含靶多核苷酸的液体接触。 在接触步骤中，相对于电极表面固定的至少一些探针分子与其分离。 获得指示相对于电极表面固定的探针分子的量的第二电化学信号。 至少部分地基于第一和第二电化学信号确定靶多核苷酸的存在。
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4.发明申请公开(公告)号：US20110039345A1
公开(公告)日：2011-02-17
申请号：US12910112
申请日：2010-10-22
申请人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
发明人： John S. Althaus , Lee Kyonghoon , Vijay Namasivayam , Sundaresh N. Brahmasandra , Kalyan Handique
IPC分类号： G01N33/53
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N33/5438 , Y10T436/143333 , C12Q2565/607 , C12Q2563/173
摘要： The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reversibly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.
摘要翻译： 本发明涉及一种用于检测靶多核苷酸的电化学方法。 提供了包括电极表面的电极。 电极表面包括相对于电极表面可逆地固定的至少一个探针分子。 获得指示相对于电极表面固定的探针分子的量的第一电化学信号。 电极表面与包含靶多核苷酸的液体接触。 在接触步骤中，相对于电极表面固定的至少一些探针分子与其分离。 获得指示相对于电极表面固定的探针分子的量的第二电化学信号。 至少部分地基于第一和第二电化学信号确定靶多核苷酸的存在。
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