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公开(公告)号：US08324372B2
公开(公告)日：2012-12-04
申请号：US12172214
申请日：2008-07-11
IPC分类号： C07H21/00
CPC分类号： C12N15/1013 , C07H1/08 , C12Q1/6813
摘要： Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamidoamine (PAMAM(Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.
摘要翻译： 用于处理含多核苷酸的生物样品的方法，以及从这些样品中捕获多核苷酸分子例如RNA和/或DNA的材料。 RNA和/或DNA被结合到表面的聚酰胺胺（PAMAM（代0））捕获，例如磁性颗粒的表面。 所述方法和材料具有高的结合RNA和DNA的效率，并具有释放，从而允许定量测定。
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2.发明申请公开(公告)号：US20080182301A1
公开(公告)日：2008-07-31
申请号：US11985577
申请日：2007-11-14
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/10 , B01L2200/147 , B01L2200/148 , B01L2200/16 , B01L2300/021 , B01L2300/045 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0611 , B01L2400/0677 , B01L2400/0683 , C12Q1/686 , F16K99/0001 , F16K99/003 , F16K99/0032 , F16K99/0044 , F16K99/0061 , F16K2099/0084 , G01N21/6428 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2035/00881
摘要： The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
摘要翻译： 本技术提供了用于检测样品中特别是生物样品中多核苷酸的装置。 该技术更特别涉及在微流体通道内对感兴趣的核苷酸进行PCR并检测这些核苷酸的微流体系统。 该装置包括被配置为接受多个样品并且可以分别对每个样品或一组或多个样品同时进行PCR的微流控盒。
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公开(公告)号：USD565742S1
公开(公告)日：2008-04-01
申请号：US29263444
申请日：2006-07-21
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4.发明授权Integrated system for processing microfluidic samples, and method of using the same 有权用于加工微流体样品的集成系统及其使用方法公开(公告)号：US09040288B2
公开(公告)日：2015-05-26
申请号：US11728964
申请日：2007-03-26
申请人： Kalyan Handique , Sundaresh N. Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
发明人： Kalyan Handique , Sundaresh N. Brahmasandra , Karthik Ganesan , Betty Wu , Nikhil Phadke , Gene Parunak , Jeff Williams
CPC分类号： B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/10 , B01L2200/147 , B01L2200/148 , B01L2200/16 , B01L2300/021 , B01L2300/06 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/18 , B01L2300/1827 , B01L2300/1861 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0611 , B01L2400/0677 , B01L2400/0683 , F16K99/0001 , F16K99/003 , F16K99/0032 , F16K99/0044 , F16K99/0061 , F16K2099/0084 , G01N2035/00881
摘要： This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.
摘要翻译： 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。
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公开(公告)号：US08883490B2
公开(公告)日：2014-11-11
申请号：US11940321
申请日：2007-11-14
CPC分类号： B01L7/52 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0684 , B01L2300/021 , B01L2300/022 , B01L2300/045 , B01L2300/046 , B01L2300/0861 , B01L2300/1805 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2300/1861 , C12Q1/686 , G01N21/6428 , G01N2021/6419 , Y10S435/808 , C12Q2565/629
摘要： The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
摘要翻译： 本技术提供了一种荧光检测器，其被配置为检测针对多核苷酸的特征性探针发射的光。 多核苷酸正在微流体通道中进行扩增，通过该微流体通道，检测器与光通信。 检测器被配置为检测微量的多核苷酸，例如将包含在微流体体积中。 检测器也可以被多路复用以允许多个同时测量多个多核苷酸并发测量。
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6.发明授权公开(公告)号：US07998708B2
公开(公告)日：2011-08-16
申请号：US11985577
申请日：2007-11-14
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/10 , B01L2200/147 , B01L2200/148 , B01L2200/16 , B01L2300/021 , B01L2300/045 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0442 , B01L2400/0481 , B01L2400/0487 , B01L2400/0611 , B01L2400/0677 , B01L2400/0683 , C12Q1/686 , F16K99/0001 , F16K99/003 , F16K99/0032 , F16K99/0044 , F16K99/0061 , F16K2099/0084 , G01N21/6428 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2035/00881
摘要： The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
摘要翻译： 本技术提供了用于检测样品中特别是生物样品中多核苷酸的装置。 该技术更特别涉及在微流体通道内对感兴趣的核苷酸进行PCR并检测这些核苷酸的微流体系统。 该装置包括被配置为接受多个样品并且可以分别对每个样品或一组或多个样品同时进行PCR的微流控盒。
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公开(公告)号：US20100009351A1
公开(公告)日：2010-01-14
申请号：US12172208
申请日：2008-07-11
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006
摘要： Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as DNA from such samples. The DNA is captured by polyethyeleneimine (PEI) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding DNA and of release, and thereby permit quantitative determinations.
摘要翻译： 用于处理含有多核苷酸的生物样品的方法，以及用于从这些样品中捕获多核苷酸分子例如DNA的材料。 DNA被结合到表面的聚乙烯亚胺（PEI）捕获，例如磁性颗粒的表面。 所述方法和材料具有高的结合DNA和释放的效率，从而允许定量测定。
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公开(公告)号：US20080262213A1
公开(公告)日：2008-10-23
申请号：US11579353
申请日：2005-05-03
CPC分类号： C12Q1/686 , B01F5/0646 , B01F5/0647 , B01F13/0059 , B01L3/50273 , B01L3/502738 , B01L3/502753 , B01L3/5082 , B01L3/565 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0442 , B01L2400/0677 , B01L2400/0683 , B01L2400/0694 , C12N15/1006 , C12Q1/6806 , F16K99/0001 , F16K99/0019 , F16K99/0032 , F16K99/0036 , F16K99/0044 , F16K2099/0084 , C12Q2565/629
摘要： Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
摘要翻译： 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下重新获得多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如，较高的温度和/ 或更多的碱性pH）。 处理区域可用于例如浓缩样品的多核苷酸和/或来自多核苷酸的分离的扩增反应抑制剂。 公开了具有处理区域的微流体装置。
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公开(公告)号：US20080149840A1
公开(公告)日：2008-06-26
申请号：US11940321
申请日：2007-11-14
IPC分类号： G01T1/16
CPC分类号： B01L7/52 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0684 , B01L2300/021 , B01L2300/022 , B01L2300/045 , B01L2300/046 , B01L2300/0861 , B01L2300/1805 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2300/1861 , C12Q1/686 , G01N21/6428 , G01N2021/6419 , Y10S435/808 , C12Q2565/629
摘要： The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
摘要翻译： 本技术提供了一种荧光检测器，其被配置为检测针对多核苷酸的特征性探针发射的光。 多核苷酸正在微流体通道中进行扩增，通过该微流体通道，检测器与光通信。 检测器被配置为检测微量的多核苷酸，例如将包含在微流体体积中。 检测器也可以被多路复用以允许多个同时测量多个多核苷酸并发测量。
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公开(公告)号：USD566291S1
公开(公告)日：2008-04-08
申请号：US29257030
申请日：2006-03-27
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