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1.发明申请METHOD FOR RAPID IDENTIFICATION OF MOLECULES WITH FUNCTIONAL ACTIVITY TOWARDS BIOMOLECULAR TARGETS 审中-公开具有功能活性的生物分子靶标快速鉴定分子的方法公开(公告)号：US20070264661A1
公开(公告)日：2007-11-15
申请号：US11774463
申请日：2007-07-06
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/573
CPC分类号： C12Q1/68 , G01N33/6848 , C12Q2565/627
摘要： The present invention provides mass spectrometry-based methods for high throughput identification of molecules with functional activities such as nuclease, protease, reductase, kinase, phosphatase, or transferase activities towards biomolecular targets. These methods are useful for screening biological samples such as extracts, broths, lysates and natural product mixtures and provide valuable insights into biomolecular interactions of various biological ligands with biomolecular targets.
摘要翻译： 本发明提供基于质谱法的高通量鉴定具有功能活性如分子核酸酶，蛋白酶，还原酶，激酶，磷酸酶或转移酶活性的生物分子靶标的分子。 这些方法可用于筛选生物样品如提取物，肉汤，裂解物和天然产物混合物，并为各种生物配体与生物分子靶标的生物分子相互作用提供有价值的见解。
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2.发明申请Method for rapid identification of molecules with functional activity towards biomolecular targets 审中-公开快速鉴定具有生物分子靶标功能活性的分子的方法公开(公告)号：US20050095622A1
公开(公告)日：2005-05-05
申请号：US10922739
申请日：2004-08-20
IPC分类号： C07H21/02 , C12Q1/68 , G01N20060101 , G01N33/68
CPC分类号： C12Q1/68 , G01N33/6848 , C12Q2565/627
摘要： The present invention provides mass spectrometry-based methods for high throughput identification of molecules with functional activities such as nuclease, protease, reductase, kinase, phosphatase, or transferase activities towards biomolecular targets. These methods are useful for screening biological samples such as extracts, broths, lysates and natural product mixtures and provide valuable insights into biomolecular interactions of various biological ligands with biomolecular targets.
摘要翻译： 本发明提供基于质谱法的高通量鉴定具有功能活性如分子核酸酶，蛋白酶，还原酶，激酶，磷酸酶或转移酶活性的生物分子靶标的分子。 这些方法可用于筛选生物样品如提取物，肉汤，裂解物和天然产物混合物，并为各种生物配体与生物分子靶标的生物分子相互作用提供有价值的见解。
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3.发明申请Systems and method of a gated electrospray interface with variable flow rate for high throughput mass spectrometric analysis 有权具有可变流速的门控电喷雾界面的系统和方法，用于高通量质谱分析公开(公告)号：US20050029447A1
公开(公告)日：2005-02-10
申请号：US10939753
申请日：2004-09-13
CPC分类号： H01J49/04
摘要： The present disclosure is related to improved systems and methods for delivering samples for high-throughput mass spectrometric analysis to an atmospheric-pressure ionization source. In an exemplary embodiment, the system includes a solvent reservoir for storing a solvent solution, a first valve which is coupled to the solvent reservoir, first and second pumps for delivering solvent solution and which are coupled to the first valve and which the delivery flow rate of the first pump is greater than the delivery flow rate of the second pump, an injection system having a sample injector and an second valve which is coupled to the first valve and which is capable of being coupled to can be couple to an electrospray ionization source. In another embodiment, the system can also include an atmospheric-pressure ionization chamber, an atmospheric-pressure ionization sprayer and a nebulizer gas source and a voltage supply source. In yet another embodiment, the system may further include a puffer valve that is coupled to the nebulizer gas source and the atmospheric-pressure ionization sprayer and a gas puffer that is coupled to the puffer valve. A distal end of the gas puffer may be located within the atmospheric-pressure ionization chamber and aligned with the distal end of the atmospheric-pressure ionization sprayer and the puffer valve may control the delivery of the nebulizer gas to the atmospheric-pressure ionization sprayer and the gas puffer.
摘要翻译： 本公开涉及用于将用于高通量质谱分析的样品递送到大气压电离源的改进的系统和方法。 在示例性实施例中，系统包括用于存储溶剂溶液的溶剂储存器，耦合到溶剂储存器的第一阀，用于输送溶剂溶液的第一和第二泵，并且其耦合到第一阀，并且输送流量 的第一泵的输送流量大于第二泵的输送流量，具有样品注射器的注射系统和耦合到第一阀并且能够耦合的第二阀可以耦合到电喷雾离子源 。 在另一个实施例中，系统还可以包括大气压电离室，大气压电离喷雾器和喷雾器气体源和电压源。 在另一个实施例中，该系统还可以包括与喷雾器气体源和大气压电离喷雾器相连的吹气阀以及耦合到吹气阀的气体吹气器。 气体吹气器的远端可以位于大气压电离室内并与大气压电离喷雾器的远端对准，并且吹气阀可以控制喷雾器气体输送到大气压电离喷雾器， 气体吹气
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4.发明申请Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture 有权用于通过溶液捕获的质谱法快速纯化核酸以进行后续分析的方法公开(公告)号：US20050130196A1
公开(公告)日：2005-06-16
申请号：US10943344
申请日：2004-09-17
申请人： Steven Hofstadler , Lendell Cummins
发明人： Steven Hofstadler , Lendell Cummins
CPC分类号： C12Q1/6806 , C12N15/101 , C12N15/1013 , H01J49/165 , C12Q2563/155 , C12Q2563/143 , C12Q2565/627
摘要： The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
摘要翻译： 本发明提供了一种用于快速溶液捕获纯化核酸的方法，用于随后通过电喷雾质谱法进行分析，其相对于现有方法是有效且成本有效的。 本发明还提供了用于实施核酸的快速溶液捕获的试剂盒，使得纯化的样品处于通过电喷雾质谱分析的条件下。
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5.发明申请Methods for rapid purification of nucleic acids for subsquent analysis by mass spectrometery by solution capture 有权通过溶液捕获通过质谱法快速纯化核酸进行次级分析的方法公开(公告)号：US20050164215A1
公开(公告)日：2005-07-28
申请号：US10844938
申请日：2004-05-12
申请人： Steven Hofstadler , Lendell Cummins
发明人： Steven Hofstadler , Lendell Cummins
CPC分类号： C12N15/101 , C12Q1/6806 , C12Q2563/155 , C12Q2563/143 , C12Q2565/627
摘要： The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
摘要翻译： 本发明提供了一种用于快速溶液捕获纯化核酸的方法，用于随后通过电喷雾质谱法进行分析，其相对于现有方法是有效且成本有效的。 本发明还提供了用于实施核酸的快速溶液捕获的试剂盒，使得纯化的样品处于用于通过电喷雾质谱分析的条件。
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6.发明申请公开(公告)号：US20070048735A1
公开(公告)日：2007-03-01
申请号：US10660997
申请日：2003-09-12
申请人： David Ecker , Richard Griffey , Rangarajan Sampath , Steven Hofstadler , John McNeil , Stanley Crooke , James Hannis
发明人： David Ecker , Richard Griffey , Rangarajan Sampath , Steven Hofstadler , John McNeil , Stanley Crooke , James Hannis
CPC分类号： C12Q1/689 , C12Q1/6813 , C12Q1/6858 , C12Q1/686 , C12Q1/6888 , C12Q2600/156 , Y02A50/59 , Y02A50/60 , Y02A90/24 , C12Q2565/627 , C12Q2545/113 , C12Q2563/167
摘要： The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents.
摘要翻译： 本发明提供了通过识别与生物武器相关的生物标签和恐怖主义或犯罪行为进行快速法医调查的方法。 该方法也可用于通过基因分型生物学的流行病学调查。
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公开(公告)号：US20050270191A1
公开(公告)日：2005-12-08
申请号：US11136134
申请日：2005-05-24
申请人： Steven Hofstadler , Jared Drader , Amy Schink
发明人： Steven Hofstadler , Jared Drader , Amy Schink
CPC分类号： H01J49/0036 , G01N33/6848 , H01J49/0009 , H01J49/0027 , H01J49/02 , H01J49/165 , H01J49/26 , H01J49/40
摘要： The methods described herein generally relate to characterization of large analytes, such as biomolecules, by molecular mass analysis. Specifically, the methods are directed to molecular mass analysis of singly- or multiply-charged ions by selective ion filtering carried out by a digital thresholding process.
摘要翻译： 本文描述的方法通常涉及通过分子量分析来表征大分析物，例如生物分子。 具体地说，该方法涉及通过数字阈值处理进行的选择性离子滤波来对单或多电荷离子进行分子质量分析。
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8.发明申请Molecular interaction sites of 16S ribosomal RNA and methods of modulating the same 审中-公开16S核糖体RNA的分子相互作用位点及其调控方法公开(公告)号：US20050250133A1
公开(公告)日：2005-11-10
申请号：US11070519
申请日：2005-03-01
申请人： David Ecker , Steven Hofstadler , Richard Griffey , Rangarajan Sampath , Kristin Lowery , Christian Massire
发明人： David Ecker , Steven Hofstadler , Richard Griffey , Rangarajan Sampath , Kristin Lowery , Christian Massire
CPC分类号： C12N15/11
摘要： Polynucleotides comprising molecular interaction sites of 16S rRNA that have particular secondary structure are provided. Methods of using such polynucleotides to screen, virtually or actually, combinatorial libraries of compounds that bind thereto are also provided. Method of modulating the activity of 16S rRNA by contacting 16S rRNA or prokaryotic cells containing the same with a compound identified by such virtual or actual screening are also provided.
摘要翻译： 提供了包含具有特定二级结构的16S rRNA的分子相互作用位点的多核苷酸。 还提供了使用这样的多核苷酸筛选，实际上或实际上筛选结合其的化合物的组合文库的方法。 还提供了通过将含有该酶的16S rRNA或原核细胞与通过这种虚拟或实际筛选鉴定的化合物接触来调节16S rRNA活性的方法。
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公开(公告)号：US06656690B2
公开(公告)日：2003-12-02
申请号：US09884317
申请日：2001-06-19
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , B01J2219/00702 , B01J2219/00722 , C12Q1/6806 , C12Q1/6811 , C40B40/06 , H01J49/004 , Y10T436/24 , C12Q2565/627 , C12Q2525/101 , C12Q2565/133 , C12Q2525/121 , C12Q2565/607
摘要： The present invention provides methods for the determination of the structure of biomolecular targets, as well as the site and nature of the interaction between ligands and biomolecular targets. The present invention also provides methods for the determination of the relative affinity of a ligand for the biomolecular target it interacts with. Also provided are methods for screening ligand or combinatorial libraries of compounds against one or more than one biological target molecules. The methods of the invention also allow determination of the relative binding affinity of combinatorial and other compounds for a biomolecular target. The present invention further provides methods for the use of mass modifying tags for screening multiple biomolecular targets. In a preferred embodiment, ligands which have great specificity and affinity for molecular interaction sites on biomolecules, especially RNA can be identified. In preferred embodiments, such identification can be made simultaneously with libraries of ligands.
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10.发明申请Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby 审中-公开定义数据库的二级结构和用于确定未知生物标签的身份和地理起源的方法公开(公告)号：US20050027459A1
公开(公告)日：2005-02-03
申请号：US10439706
申请日：2003-05-16
申请人： David Ecker , Richard Griffey , Rangarajan Sampath , Steven Hofstadler , John McNeil , Stanley Crooke
发明人： David Ecker , Richard Griffey , Rangarajan Sampath , Steven Hofstadler , John McNeil , Stanley Crooke
IPC分类号： A61B20060101 , C12P19/34 , C12Q1/68 , C12Q1/70 , G01N31/00 , G01N33/48 , G01N33/50 , G06F19/00 , G06F19/18
摘要： The present invention relates generally to the field of investigational bioinformatics and more particularly to secondary structure defining databases. The present invention further relates to methods for interrogating a database as a source of molecular masses of known bioagents for comparing against the molecular mass of an unknown or selected bioagent to determine either the identity of the selected bioagent, and/or to determine the origin of the selected bioagent. The identification of the bioagent is important for determining a proper course of treatment and/or irradication of the bioagent in such cases as biological warfare. Furthermore, the determination of the geographic origin of a selected bioagent will facilitate the identification of potential criminal identity.
摘要翻译： 本发明一般涉及研究生物信息学领域，更具体地涉及定义数据库的二级结构。 本发明还涉及询问数据库作为已知生物标签的分子量来源的方法，用于与未知或选择的生物标签的分子量进行比较以确定所选择的生物标签的身份，和/或确定所选生物标记的来源 所选的生物标签。 生物制剂的鉴定对于在诸如生物战争的情况下确定生物制剂的适当治疗过程和/或辐照是重要的。 此外，确定所选择的生物制剂的地理起源将有助于确定潜在的犯罪认同。
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