公开(公告)号：US20070134684A1

公开(公告)日：2007-06-14

申请号：US11457713

申请日：2006-07-14

Applicant: Dmitri Petrovykh ,  Lloyd Whitman ,  Michael Tarlov ,  Aric Opdahl ,  Hiromi Kimura-Suda

Inventor： Dmitri Petrovykh ,  Lloyd Whitman ,  Michael Tarlov ,  Aric Opdahl ,  Hiromi Kimura-Suda

IPC: C12Q1/68 ,  C12M1/34 ,  B05D3/00

CPC classification number: B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00572 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01J2219/0074 ,  B01J2219/00743

Abstract: The method provides for attaching nucleic acids to a surface at a controlled grafting density in a controlled conformation by contacting an immobilization solution of nucleic acids containing at least one block of adenine nucleotides to a surface for a sufficient period of time to allow attachment to the surface. Another aspect of the methods described provides for controlling the grafting density of immobilized oligonucleotides by coadsorption/displacement by oligo(dA). Another aspect provides for a method of immobilizing oligonucleotides in complex conformations by varying the number and position of the block(s) of adenine nucleotides in the sequence of said oligonucleotides. Another aspect provides for controlled immobilization of a functional unit, such as a ligand, a molecule, a macromolecule, an aptamer, a lectin, an immunoglobulin, an antibody, a biomolecule, a solid state particle, a vesicle, or a label to a surface via attachment to at least one block of adenine nucleotides.

Abstract translation: 该方法提供以受控构象的受控接枝密度将核酸附着到表面，通过将含有至少一个腺嘌呤核苷酸嵌段的核酸的固定化溶液与表面接触足够的时间以允许附着于表面 。 所述方法的另一方面提供了通过寡聚（dA）的共吸附/置换来控制固定的寡核苷酸的接枝密度。 另一方面提供了通过改变所述寡核苷酸序列中腺嘌呤核苷酸嵌段的数目和位置来固定复合构象中的寡核苷酸的方法。 另一方面提供功能单元如配体，分子，大分子，适体，凝集素，免疫球蛋白，抗体，生物分子，固态颗粒，囊泡或标签的受控固定化 通过连接至至少一个腺嘌呤核苷酸区的表面。

公开(公告)号：US20060040057A1

公开(公告)日：2006-02-23

申请号：US10956596

申请日：2004-09-29

Applicant: Paul Sheehan ,  Lloyd Whitman ,  William King

Inventor： Paul Sheehan ,  Lloyd Whitman ,  William King

IPC: B05D5/00 ,  B05C11/00

CPC classification number: G03F7/0002 ,  G01Q80/00 ,  Y10S977/849 ,  Y10S977/851 ,  Y10S977/855 ,  Y10S977/857

Abstract: The present invention describes an apparatus for nanolithography and a process for thermally controlling the deposition of a solid organic “ink” from the tip of an atomic force microscope to a substrate. The invention may be used to turn deposition of the ink to the substrate on or off by either raising its temperature above or lowing its temperature below the ink's melting temperature. This process may be useful as it allows ink deposition to be turned on and off and the deposition rate to change without the tip breaking contact with the substrate. The same tip can then be used for imaging purposes without fear of contamination. This invention can allow ink to be deposited in a vacuum enclosure, and can also allow for greater spatial resolution as the inks used have lower surface mobilities once cooled than those used in other nanolithography methods.

公开(公告)号：US20060286803A1

公开(公告)日：2006-12-21

申请号：US11424643

申请日：2006-06-16

Applicant: Christina Cole ,  Lloyd Whitman

Inventor： Christina Cole ,  Lloyd Whitman

IPC: H01L21/44

CPC classification number: B81C1/00206 ,  B81B2201/06

Abstract: Provided is a method for controllably activating a surface for stable amine-reactive chemistries. A surface containing nitride is exposed to a plasma having a reactive species containing hydrogen for a period of time sufficient to activate the substrate for amine-reactive chemistries. Amine-reactive chemical processes can then be applied to the activated surface to reliably and controllably bond molecules directly to said surface. The method is designed to create stable primary amines on the nitride substrate, so that any subsequent amine-reactive chemistry may proceed in a controlled manner that is directly proportional to the density of surface amines so created.

Abstract translation: 提供了一种用于可控地激活用于稳定的胺反应性化学物质的表面的方法。 将含有氮化物的表面暴露于具有含氢活性物质的等离子体的时间足以激活用于胺反应性化学物质的底物。 然后可以将胺反应性化学过程施加到活化表面，以可靠和可控地将分子直接结合到所述表面。 该方法被设计成在氮化物衬底上产生稳定的伯胺，使得任何随后的胺反应性化学物质可以以如此产生的表面胺的密度成正比的受控方式进行。