公开(公告)号：US5436145A

公开(公告)日：1995-07-25

申请号：US246570

申请日：1994-05-20

申请人： Mari Yasuda ,  Haruyuki Ohkishi ,  Katsutoshi Sato ,  Yuuki Morimoto ,  Toru Nagasawa

发明人： Mari Yasuda ,  Haruyuki Ohkishi ,  Katsutoshi Sato ,  Yuuki Morimoto ,  Toru Nagasawa

IPC分类号： C12P17/06 ,  C12P17/12 ,  C12R1/01 ,  C12R1/06

CPC分类号： C12P17/12 ,  Y10S435/822 ,  Y10S435/824 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/847 ,  Y10S435/85 ,  Y10S435/859 ,  Y10S435/874 ,  Y10S435/876 ,  Y10S435/877 ,  Y10S435/88 ,  Y10S435/881

摘要： 6-Hydroxy nitrogen-containing 6-membered ring compounds of the following general formula (II): ##STR1## wherein R.sup.1 represents carboxy group, carbamoyl group, cyano group, formyl group, C.sub.1 -C.sub.5 hydroxyalkyl group, C.sub.2 -C.sub.6 alkoxycarbonyl group, carboxyvinyl group, carboxymethyl group or oxime group, R.sup.2 represents hydrogen atom or carboxy group, and A represents carbon atom or nitrogen atom, can be prepared by reacting a nitrogen-containing 6-membered ring compounds of the following general formula (I): ##STR2## wherein R.sup.1, R.sup.2 and A are as defined in the general formula (II) above, with a microorganism or physico-chemically treated microorganism in an aqueous medium. Efficiency of the above reaction can be raised by conducting the reaction in the presence of phenazine methosulfate.

摘要翻译： 下述通式（II）的6-羟基含氮6元环化合物：其中R 1表示羧基，氨基甲酰基，氰基，甲酰基，C 1 -C 5羟基烷基，C 2 -C 6 烷氧基羰基，羧基乙烯基，羧甲基或肟基，R2表示氢原子或羧基，A表示碳原子或氮原子，可以通过使下述通式（I）的含氮6元环化合物 ）：其中R 1，R 2和A如上述通式（II）中所定义，其在水性介质中与微生物或物理化学处理的微生物一起使用。 可以通过在吩嗪甲硫酸盐存在下进行反应来提高上述反应的效率。

公开(公告)号：US5700670A

公开(公告)日：1997-12-23

申请号：US630623

申请日：1996-04-10

申请人： Masahiro Yamagishi ,  Makoto Ueda ,  Yukie Takai ,  Mari Yasuda ,  Takashi Mikawa

发明人： Masahiro Yamagishi ,  Makoto Ueda ,  Yukie Takai ,  Mari Yasuda ,  Takashi Mikawa

IPC分类号： C07C69/675 ,  C07C227/16 ,  C07C229/22 ,  C07C255/19 ,  C12P7/62 ,  C12P13/00 ,  C12R1/645

CPC分类号： C12R1/645 ,  C12P7/62 ,  Y10S435/911

摘要： Microbial cells and/or a preparation therefrom of a microorganism is allowed to act on an ester of .gamma.-substituted-acetoacetic acid, and a carbonyl group at its .beta.-position is stereospecifically reduced, wherein the microorganism is selected from the group consisting of those belonging to the genera Yarrowia, Filobasidium, Metschnikowia, Galactomyces, Ambrosiozyma, Trichosporonoides, Aureobasidium, Phaeococcomyces, Rosulomyces, Dothichiza, Emericellopsis, Calonectria, Colletotrichum, and Ceratocystis, preferably, the microorganism is microorganism belonging to the genus Aureobasidium. Thus an optically active ester of .gamma.-substituted-.beta.-hydroxybutyric acid having a high optical purity is produced in a short period of time at a high accumulated concentration and a high yield.

摘要翻译： 微生物细胞和/或其微生物的制剂被允许作用于γ-取代的乙酰乙酸的酯上，其β-位上的羰基被立体选择性地还原，其中微生物选自那些 属于耶氏酵母属（Yarrowia），云杉属（Filobasidium），麦草属（Metschnikowia），半乳杆菌属（Galactomyces），双孢蘑菇（Trichosporonoides），白尾翁属（Aureobasidium），球孢酵母属（Phaeococcomyces），Rosulomyces，Dothichiza，Emericellopsis，Calonectria，Colletotrichum和Ceratocystis，优选为属于Aureobasidium属的微生物。 因此，在高浓度和高产率下，在短时间内产生具有高光学纯度的γ-取代-β-羟基丁酸的光学活性酯。

公开(公告)号：US5841001A

公开(公告)日：1998-11-24

申请号：US808853

申请日：1997-02-28

申请人： Ken Tanaka ,  Mari Yasuda ,  Makoto Ueda

发明人： Ken Tanaka ,  Mari Yasuda ,  Makoto Ueda

IPC分类号： C07C213/04 ,  C07D301/26 ,  C07D303/08 ,  C12P7/22 ,  C12P41/00 ,  C07C209/02 ,  C07C33/20 ,  C07C33/34

CPC分类号： C07C213/04 ,  C07D301/26 ,  C07D303/08 ,  C12P41/004 ,  C12P7/22

摘要： The present invention provides an industrially advantageous process for the preparation of an optically active 2-halo-1-(substituted phenyl)ethanol useful as medicines, agricultural chemicals or as intermediates thereof; and a simple process for the preparation of an optically active substituted styrene oxide or 2-amino-1-(substituted phenyl)ethanol useful as medicines, agricultural chemicals or intermediates thereof. A 2-halo-1-(substituted phenyl)ethanol represented by the following general formula (I) is allowed to contact, in the presence of a carboxylic anhydride, with an enzyme stereoselectively catalyzing ester interchange to produce an optically active 2-halo-1-(substituted phenyl)ethanol: ##STR1## (wherein X represents a chlorine atom or a bromine atom, and R.sup.1, R.sup.2 and R.sup.3 may be the same or different and each represent a hydrogen atom, a halogen atom, a C.sub.1-5 alkyl group, a C.sub.1-5 haloalkyl group, a C.sub.1-5 alkoxy group, a cyano group or a nitro group, with the proviso that when two of R.sup.1, R.sup.2 and R.sup.3 are alkyl groups or alkoxy groups, they may be combined together to form a ring and that all of R.sup.1, R.sup.2 and R.sup.3 are not hydrogen atoms at the same time).

摘要翻译： 本发明提供用于制备用作药物，农药或其中间体的光学活性2-卤代-1-（取代苯基）乙醇的工业上有利的方法; 以及用作制备用作药物，农药或其中间体的光学活性取代的氧化苯乙烯或2-氨基-1-（取代的苯基）乙醇的简单方法。 使由以下通式（I）表示的2-卤代-1-（取代的苯基）乙醇在羧酸酐的存在下与酶立体选择性催化酯交换反应以产生光学活性的2-卤代 - 1-（取代苯基）乙醇：（I）（其中X表示氯原子或溴原子，R 1，R 2和R 3可以相同或不同，表示氢原子，卤素原子， C 1-5烷基，C 1-5卤代烷基，C 1-5烷氧基，氰基或硝基，条件是当R 1，R 2和R 3中的两个是烷基或烷氧基时，它们可以是 组合在一起形成环，并且所有的R 1，R 2和R 3不同时为氢原子）。