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47 条记录 (耗时 0.016 秒)

    Method of selectively determining a fungal biomass
    1.
    发明授权
    Method of selectively determining a fungal biomass 有权
    选择性测定真菌生物量的方法

    公开(公告)号：US06372446B1

    公开(公告)日：2002-04-16

    申请号：US09355790

    申请日：1999-08-03

    申请人： Morten Miller ,  Morten Reeslev

    发明人： Morten Miller ,  Morten Reeslev

    IPC分类号： G01N3353

    CPC分类号： G01N33/573 ,  C12Q1/34 ,  C12Q2334/22 ,  G01N2333/37 ,  G01N2333/924

    摘要： A method for selectively determining a fungal biomass by detection of a fungal enzymatic activity that is present in substantially all fungal species, such as enzymes involved in chitin metabolism (chitinase, chitin synthase, chitosanase, N-acetyl-glucosaminidase and &bgr;-N-acetylhexosamidase). The invention can be used for detecting a fungal biomass in environmental samples, food products, plant material, building materials, industrial fungal cultures or sample from a human being or animal including blood samples. In particular, 4-methylumbelliferyl-N-acetyl-&bgr;-D-glucosaminide is used as substrate for &bgr;-N-acetylhexosamidase (EC 3.2.1.52). This enzyme activity correlates with the amount of fungal biomass present in a sample.

    摘要翻译： 通过检测存在于基本上所有真菌物质中的真菌酶活性（例如参与甲壳素代谢的酶（几丁质酶，几丁质合酶，壳聚糖酶，N-乙酰氨基葡糖苷酶和β-N-乙酰基己糖酰胺酶 ）。 本发明可用于检测环境样品，食品，植物材料，建筑材料，工业真菌培养物或包括血液样品在内的人或动物的样品中的真菌生物量。 特别地，使用4-甲基伞形酮-N-乙酰基-β-D-氨基葡糖苷作为β-N-乙酰基己糖酰胺酶的底物（EC 3.2.1.52）。 该酶活性与样品中存在的真菌生物量的量相关。

    Rapid coliform detection system
    2.
    发明授权
    Rapid coliform detection system 失效
    快速大肠菌群检测系统

    公开(公告)号：US6165742A

    公开(公告)日：2000-12-26

    申请号：US373401

    申请日：1999-08-12

    申请人： Gro .O slashed.fjord ,  Kari Skj.ang.nes ,  Nina Aalen ,  James D. Berg

    发明人： Gro .O slashed.fjord ,  Kari Skj.ang.nes ,  Nina Aalen ,  James D. Berg

    IPC分类号： C12Q1/10 ,  C12Q1/04 ,  C12P1/00 ,  C12Q1/02 ,  C12Q1/54

    CPC分类号： C12Q1/10 ,  C12Q2304/00 ,  C12Q2334/22 ,  G01N2333/245 ,  Y10S435/968

    摘要： A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example skimmed milk. A growth medium containing a fluorogenic substrate is combined with the sample and is incubated for a brief period of about 7-9 hours after which a single fluorescence value is measured. Total or thermotolerant coliform bacteria are determined to be present in the sample if the single fluorescent measurement exceeds a predetermined threshold value.

    摘要翻译： 用于检测液体或液化乳制品样品中大肠杆菌存在或不存在的快速方法，例如脱脂乳。 将含有荧光底物的生长培养基与样品组合，并孵育约7-9小时的短时间，之后测量单个荧光值。 如果单个荧光测量超过预定阈值，则确定总样本或耐热大肠菌群存在于样品中。

    Enzyme substrate
    3.
    发明授权
    Enzyme substrate 失效
    酶底物

    公开(公告)号：US5895819A

    公开(公告)日：1999-04-20

    申请号：US881360

    申请日：1997-06-24

    申请人： Norio Hagi

    发明人： Norio Hagi

    IPC分类号： C12Q1/42 ,  C07F9/06 ,  C07F9/22

    CPC分类号： C12Q1/42 ,  C12Q2334/22

    摘要： An enzyme substrate is disclosed which comprises a phosphoric ester and an organic acid or a salt thereof having a zinc chelating stability factor constant (log K) of from 8 to 14 in an aqueous solution having an ionic strength of 0.1 at 20 to 25.degree. C. A reagent kit for an enzyme activity assay, a reagent kit for an immunoassay, a method for stabilizing a phosphoric ester, a method for producing a stabilized phosphoric ester.

    摘要翻译： 公开了一种酶底物，其在20〜25℃的离子强度为0.1的水溶液中含有磷酸酯和有机酸或其盐，其螯合稳定因子常数（logK）为8〜14 用于酶活性测定的试剂盒，免疫测定用试剂盒，磷酸酯稳定化方法，稳定化磷酸酯的制造方法。

    Enzyme-capture assay (ECA) for the identification of Escherichia coli in clinical samples
    5.
    发明授权
    Enzyme-capture assay (ECA) for the identification of Escherichia coli in clinical samples 失效
    用于在临床样品中鉴定大肠杆菌的酶 - 捕获测定（ECA）

    公开(公告)号：US5612186A

    公开(公告)日：1997-03-18

    申请号：US263541

    申请日：1994-06-22

    申请人： Shiu W. Huang ,  Jiunn J. Wu ,  Tsung C. Chang

    发明人： Shiu W. Huang ,  Jiunn J. Wu ,  Tsung C. Chang

    IPC分类号： C12Q1/10 ,  G01N33/569

    CPC分类号： C12Q1/10 ,  C12Q2334/22

    摘要： An enzyme-capture assay (ECA) for rapid identification of Escherichia coli (E. coli) in clinical samples comprising contacting a clinical sample which is suspected to contain E. coli with an immobilized antibody against .beta.-D-glucuronidase which then catalyzes an enzyme substrate to produce a fluorescent product is disclosed.

    摘要翻译： 用于在临床样品中快速鉴定大肠杆菌（E.coli）的酶 - 捕获测定（ECA），包括将怀疑含有大肠杆菌的临床样品与抗β-葡糖醛酸糖苷酶的固定化抗体接触，然后催化酶 公开了生产荧光产品的底物。

    Flourescent aquatic bioassay and procedure
    7.
    发明授权
    Flourescent aquatic bioassay and procedure 失效
    鱼类水生生物和程序

    公开(公告)号：US5094944A

    公开(公告)日：1992-03-10

    申请号：US503296

    申请日：1990-04-02

    申请人： Kenneth R. Hayes

    发明人： Kenneth R. Hayes

    IPC分类号： C12Q1/18 ,  C12Q1/02 ,  C12Q1/34 ,  G01N33/18 ,  G01N33/50

    CPC分类号： G01N33/186 ,  C12Q1/025 ,  C12Q1/34 ,  G01N33/5014 ,  C12Q2334/22 ,  G01N2333/924

    摘要： The present invention relates to a novel assay for determining levels of toxicants in aqueous environments, preferably in water supplies. The present invention also relates to a method for utilizing the assay to test the level of toxicants in an aquatic source. Further embodiments of the present invention relate to a test kit embodying the assay of the present invention. More specifically the assay involves the use of enzyme substrates having an umbelliferyl group and multi-cellular organisms having bodies which fluoresce.

    摘要翻译： 本发明涉及一种用于确定水性环境中有毒物质水平的新型测定法，优选在水源中。 本发明还涉及一种利用该测定来测试水源中毒性水平的方法。 本发明的其它实施方案涉及体现本发明测定法的测试试剂盒。 更具体地，该测定涉及使用具有伞形载体的酶底物和具有发荧光的身体的多细胞生物体。

    Real time methylumbelliferone-based assay
    9.
    发明授权
    Real time methylumbelliferone-based assay 有权
    实时甲基伞形酮测定法

    公开(公告)号：US07488721B2

    公开(公告)日：2009-02-10

    申请号：US11129274

    申请日：2005-05-13

    申请人： Don Mahuran ,  Michael Tropak ,  Eric Brown

    发明人： Don Mahuran ,  Michael Tropak ,  Eric Brown

    IPC分类号： A61K31/7068 ,  H01L21/8238

    CPC分类号： C12Q1/34 ,  C12Q2334/22 ,  G01N2333/924 ,  G01N2500/00

    摘要： A method is provided for determining the activity of an enzyme which releases methylumbelliferone (MU) from an MU-containing substrate wherein the enzyme has a pH optimum below the pKa of MU comprising: contacting a sample suspected of containing the enzyme with the MU-containing substrate at a pH suitable for activity of the enzyme to allow release of MU by the enzyme; contacting the sample with light of a wavelength in the range of about 310 nm to about 350 nm; determining fluorescence produced by the released MU, thereby determining the activity of the enzyme. This real time method provides improved diagnostic methods, for example for diseases associated with an abnormal level of activity of a glycosidase. The real time assay also can be used to screen compounds for their ability to modulate enzyme activity using MU-containing substrates.

    摘要翻译： 提供了一种用于测定从含MU底物释放甲基伞形酮（MU）的酶的活性的方法，其中所述酶具有低于MU的pKa的最适pH，其包括：将怀疑含有该酶的样品与含MU 底物在适于酶的活性的pH下允许通过酶释放MU; 使样品与约310nm至约350nm的波长的光接触; 确定由释放的MU产生的荧光，从而确定酶的活性。 该实时方法提供了改进的诊断方法，例如与糖苷酶活性异常水平相关的疾病。 实时测定也可用于筛选化合物以使用含MU底物调节酶活性的能力。

    Novel potentially fluorogenic compounds and plating media containing same
    10.
    发明申请
    Novel potentially fluorogenic compounds and plating media containing same 有权
    新型潜在的含氟化合物和含有它们的电镀培养基

    公开(公告)号：US20030032080A1

    公开(公告)日：2003-02-13

    申请号：US10147323

    申请日：2002-05-17

    发明人： Gunter Schabert

    IPC分类号： C12Q001/04 ,  C07H015/00

    CPC分类号： C07F9/65522 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q2334/22 ,  C12Q2334/52 ,  G01N33/533 ,  G01N2333/195 ,  G01N2333/916 ,  Y10S435/968

    摘要： Compounds of formula (I) 1 in which R1,R2,R3,R4 and R5 are hydrogen atoms or chromogenic substituents and X is hydroxyl, OR6 wherein R6 is selected from the group consisting of C1-C4 alkyl, or OnullMenull wherein Menull is a cation derived from an organic or inorganic base; these compounds do not exhibit significant fluorescence but are capable of being cleaved by phosphatidyl-inositol-specific phospholipase C, an enzyme which is indicative of bacterial activity; the umbelliferyl moity resulting from such cleavage is a strong fluorogen thus providing effective test methods for various pathogenic bacteria, such as Listeria, Staphylococcus and Clostridium species. Also disclosed are plating media for detection of microorganisms that are capable of metabolic generation of a phosphatidyl inositol-specific phospholipase C (PInullPLC). The plating medium can be in a dry, liquid, or semi-liquid form, depending upon its water content, and comprise at least one compound capable of forming an aqueous gel when in contact with water; at least one nutrient capable of supporting growth of said microorganism; and at least one indicator compound of formula I and/or IV, notably 4-methylumbelliferyl myo-inositol-1-phosphate or salts thereof and 5-bromo-4-chloro-3-indoxyl myo-inositol-1-phosphate or salts thereof. PInullPLC generated by the microorganisms of interest leads to cleavage of the indicator compounds causing formation of fluorescence and/or color suitable for identification of type and count of such hygienically and pathologically important microorganisms as Listeria species.

    摘要翻译： 其中R 1，R 2，R 3，R 4和R 5是氢原子或显色取代基并且X是羟基的式（I）化合物，其中R 6选自C 1 -C 4烷基或O-Me +，其中Me +是 衍生自有机或无机碱的阳离子; 这些化合物不表现出显着的荧光，但能够被磷脂酰肌醇特异性磷脂酶C（一种指示细菌活性的酶）裂解; 由这种切割产生的伞形花序是强氟，从而为各种病原菌如李斯特菌，葡萄球菌和梭菌属提供有效的测试方法。 还公开了用于检测能够代谢产生磷脂酰肌醇特异性磷脂酶C（PI-PLC）的微生物的电镀培养基。 取决于其含水量，电镀介质可以是干燥，液体或半液体形式，并且包含至少一种当与水接触时能够形成水凝胶的化合物; 至少一种能够支持所述微生物生长的营养物质; 和至少一种式I和/或IV的指示剂化合物，特别是4-甲基伞形酮肌醇-1-磷酸或其盐和5-溴-4-氯-3-吲哚氧基肌醇-1-磷酸酯或其盐 。 由感兴趣的微生物产生的PI-PLC导致指示剂化合物的裂解，导致形成适合于鉴定如利斯特氏菌属的这种卫生和病理重要的微生物的类型和数量的荧光和/或颜色。