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    Circulation-active dihydropyridine ethers
    8.
    发明授权
    Circulation-active dihydropyridine ethers 失效
    循环活性二氢吡啶醚

    公开(公告)号：US4886804A

    公开(公告)日：1989-12-12

    申请号：US249391

    申请日：1988-09-20

    申请人： Gerhard Franckowiak ,  Martin Bechem ,  Rainer Gross ,  Siegfried Hebisch ,  Matthias Schramm

    发明人： Gerhard Franckowiak ,  Martin Bechem ,  Rainer Gross ,  Siegfried Hebisch ,  Matthias Schramm

    IPC分类号： A61K31/4427 ,  A61K31/455 ,  A61K31/50 ,  A61P9/00 ,  C07D211/86 ,  C07D211/90 ,  C07D401/12

    CPC分类号： C07D401/12 ,  C07D211/86 ,  C07D211/90

    摘要： Cardioactive novel dihydropyridine ethers of the formula ##STR1## in which R.sup.1 -stands for cyano, nitro or for a -COOR.sup.5 group,whereinR.sup.5 -denotes straight-chain, branched or cyclic alkyl having up to 6 carbon atoms, which can be interrupted by an oxygen in the chain and which can be monosubstituted or polysubstituted by identical or different substituents from the group consisting of halogen, cyano, hydroxyl, phenyl, phenoxy or by a group of the formula ##STR2## R.sup.2 -stands for aryl having 6 to 12 carbon atoms, which can be monosubstituted, disubstituted or trisubstituted by identical or different substituents from the group consisting of halogen, cyano, nitro, trifluoromethyl, trifluoromethoxy, trifluoromethylthio, alkyl having up to 4 carbon atoms or alkoxy having up to 4 carbon atoms,R.sup.3 -stands for aryl having 6 to 12 carbon atoms, or stands for straight-chain, branched or cyclic alkyl having up to 6 carbon atoms andR.sup.4 -stands for straight-chain alkyl having up to 4 carbon atoms or for amino or a physiologically acceptable salt thereof.

    摘要翻译： 其中R 1表示氰基，硝基或-COOR 5基团，其中R 5表示具有至多6个碳原子的直链，支链或环状烷基的式（Ⅰ）的心脏活性新型二氢吡啶醚，其可以 被链中的氧中断，并且可以被与卤素，氰基，羟基，苯基，苯氧基或下式基团相同或不同的取代基单取代或多取代：具有式 6至12个碳原子，可以被卤素，氰基，硝基，三氟甲基，三氟甲氧基，三氟甲硫基，具有至多4个碳原子的烷基或具有至多4个碳原子的烷氧基组成的组中的相同或不同的取代基单取代， 原子，具有6至12个碳原子的芳基的R3-位，或代表具有至多6个碳原子的直链，支链或环状烷基和具有至多4个碳原子的直链烷基的R 4 - 或氨基或其生理上可接受的盐。

    Masking background fluorescence and luminescence in the optical analysis of biomedical assays
    10.
    发明授权
    Masking background fluorescence and luminescence in the optical analysis of biomedical assays 有权

    公开(公告)号：US07615376B2

    公开(公告)日：2009-11-10

    申请号：US12199317

    申请日：2008-08-27

    申请人： Thomas Krahn ,  Wolfgang Paffhausen ,  Andreas Schade ,  Martin Bechem ,  Delf Schmidt

    发明人： Thomas Krahn ,  Wolfgang Paffhausen ,  Andreas Schade ,  Martin Bechem ,  Delf Schmidt

    IPC分类号： G01N21/00 ,  C12Q1/02

    CPC分类号： G01N33/542 ,  G01N33/5005 ,  G01N33/5306 ,  G01N33/54393 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/823 ,  Y10T436/13

    摘要： In a process for the quantitative optical analysis of fluorescently labelled biological cells 5, a cell layer on a transparent support at the bottom 2 of a reaction vessel 1 is in contact with a solution 3 containing the fluorescent dye 4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye 4 already present in addition a masking dye 9, which absorbs the excitation light 6 for the fluorescent dye 4 and/or its emission light 7, is added to the solution 3 and/or if a separating layer 10 permeable to the solution and absorbing and/or reflecting the excitation light 6 or the emission light 7 is applied to the cell layer at the bottom 2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer 10 must in this case be composed such that it has a high power of reflection for the luminescent light 11. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer 12 at the bottom 2 of a reaction vessel 1 is in contact with a solution (supernatant 3) in which a fluorescent or luminescent ligand 13 is dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dye 9 which absorbs the excitation light 6 for the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatant 3. Instead of the masking dye in the solution 3 or optionally as an additional measure, a separating layer 10 permeable to the solution 3 and absorbing and/or reflecting the excitation light 6 and/or the emission light or the luminescent light can be applied to the cell or receptor layer 12 at the bottom 2.