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公开(公告)号：US20130337497A1
公开(公告)日：2013-12-19
申请号：US14001996
申请日：2012-02-22
IPC分类号： G01N27/74
CPC分类号： G01N27/745 , B01L3/502761 , B01L3/502776 , B01L2200/0652 , B01L2300/0816 , B01L2400/043 , B03C1/0332 , B03C1/034 , B03C1/288 , B03C2201/26 , G01N1/40 , G01N15/1031 , G01N15/1056 , G01N33/54333 , G01N33/569 , G01N33/585 , G01N35/0098
摘要： In a measuring device, a production thereof, and a use thereof for magnetic flow cytometry, a microfluidic channel is disposed along an enrichment route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The enrichment route is thereby implemented with the microfluidic channel on the packaging of the semiconductor chip carrying the sensor. This construction ensures a long enrichment route for high throughput of large sample volumes.
摘要翻译： 在测量装置中，其生产及其用于磁流式细胞术的方法，沿着富集途径设置微流体通道，使得流过微流体通道的磁性标记的细胞样品与由磁性富集的磁导条对准 在通道的地板处的磁体的磁场，并且被引导通过传感器。 因此，在携带传感器的半导体芯片的包装上利用微流体通道实现富集途径。 这种结构确保了大量样品量的高通量的富集途径。
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公开(公告)号：US20140127710A1
公开(公告)日：2014-05-08
申请号：US14128605
申请日：2012-06-12
IPC分类号： G01N15/14 , G01N33/543
CPC分类号： G01N15/1404 , B01L3/502761 , B01L2200/0652 , B01L2400/043 , B03C1/01 , B03C1/0332 , B03C1/034 , B03C1/288 , B03C2201/18 , B03C2201/24 , B03C2201/26 , G01N15/1031 , G01N15/1056 , G01N33/54326 , Y10T29/494
摘要： The invention relates to an apparatus and a method for magnetic flow cytometry, wherein magnetic units (22, 24) are arranged in a flow channel (10) which is configured, with respect to the channel diameter (100) and the surface condition of the channel inner wall, in such a manner that a flow of a complex suspension can be produced in the flow channel (10) with a laminar flow profile (40). The forces (FM) that can be caused by the magnetic units (22, 24) and the forces (FS) that can be caused by the flow, applied to magnetic markers (26) that are not bound to cells, have the effect of holding back said magnetic markers (26) that are not bound to cells in the front channel section (240) and preventing them from continuing to flow along the flow channel (10) via the cell measuring device (20).
摘要翻译： 本发明涉及一种用于磁流式细胞术的装置和方法，其中磁性单元（22,24）布置在流道（10）中，流道（10）相对于通道直径（100）和表面状态 通道内壁，使得可以在流动通道（10）中以层流轮廓（40）产生复杂悬浮液的流动。 可以由磁性单元（22,24）引起的力（FM）和由流动引起的力（FS）施加到不与细胞结合的磁性标记（26）上的力具有 阻止所述磁性标记（26）不与前通道部分（240）中的细胞结合，并且阻止它们经由电池测量装置（20）沿着流动通道（10）继续流动。
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公开(公告)号：US20130004982A1
公开(公告)日：2013-01-03
申请号：US13172070
申请日：2011-06-29
申请人： Ludwig Bär , Oliver Hayden , Michael Johannes Helou , Mischa Megens , Mathias Reisbeck , Manfred Rührig , Sandro Francesco Tedde
发明人： Ludwig Bär , Oliver Hayden , Michael Johannes Helou , Mischa Megens , Mathias Reisbeck , Manfred Rührig , Sandro Francesco Tedde
CPC分类号： G01N33/587 , G01N15/1031 , G01N2015/1062
摘要： A magnetic flow cytometry apparatus for detection of cells labeled with magnetic nanoparticles has at least one pair of oppositely oriented magnets to provide between the magnets a first magnetic field region with a low magnetic field strength and to provide at poles of the magnets second magnetic field regions with a high magnetic field strength. The magnetic labeled cells provided within a flow input into the magnetic flow cytometry apparatus are enriched in at least one of the second magnetic field regions and supplied to the first magnetic field region, where a magnetic field is applied to the enriched magnetic labeled cells to measure the magnetic relaxation of the magnetic labeled cells in response to the applied magnetic field.
摘要翻译： 用于检测用磁性纳米颗粒标记的细胞的磁流式细胞仪装置具有至少一对相反方向的磁体，以在磁体之间提供具有低磁场强度的第一磁场区域，并且在磁体的极点处提供第二磁场区域 具有高磁场强度。 提供在输入到磁流式细胞计量装置的流量内的磁性标记细胞富集在第二磁场区域中的至少一个中，并被提供给第一磁场区域，其中向富集的磁性标记细胞施加磁场以测量 响应于施加的磁场，磁性标记的细胞的磁弛豫。
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公开(公告)号：US20140356891A1
公开(公告)日：2014-12-04
申请号：US14238943
申请日：2012-08-01
CPC分类号： G01N15/1031 , G01N33/54326 , G01N33/585 , G01N33/80 , G01N2015/0065 , G01N2015/1006 , G01R33/1269
摘要： Devices and methods for magnetic flow measurement are provided. Individual analytes are detected in the through-flow, and a dynamic detection of a changing analyte state is carried out, for example with respect to the analyte size or morphology. For this purpose, the analytes to be detected, such as cells for example, are directly marked in the medium surrounding said analytes with magnetic labels and transported through the flow channel of a measuring device comprising at least two magnetic sensors. A characteristic measurement signal is generated by means of the magnetic sensors that are mutually spaced in the flow direction. The magnetic analyte diameter is calculated using the interval between the measurement deflections, and the analyte state can be evaluated using the magnetic analyte diameter.
摘要翻译： 提供磁流测量的装置和方法。 在通流中检测到各种分析物，并且例如相对于分析物的大小或形态，进行改变的分析物状态的动态检测。 为此，待检测的分析物，例如细胞，直接用磁性标记在包围所述分析物的培养基中，并通过包含至少两个磁性传感器的测量装置的流动通道传送。 通过在流动方向上相互间隔的磁性传感器产生特征测量信号。 使用测量偏转之间的间隔计算磁分析物直径，并且可以使用磁性分析物直径来评估分析物状态。
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公开(公告)号：US20130330828A1
公开(公告)日：2013-12-12
申请号：US14002071
申请日：2012-02-28
CPC分类号： G01N15/1404 , G01N1/40 , G01N33/54333 , G01N33/569 , G01N33/585 , G01N35/0098 , H01L43/12
摘要： A measuring device for magnetic flow cytometry has a microfluidic channel disposed along an enriching route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The sensor and the magnetic guide strips are integrated on a semiconductor chip.
摘要翻译： 用于磁流式细胞术的测量装置具有沿着富集途径设置的微流体通道，使得流过微流体通道的磁标记细胞样品与通过通道底部的磁体的磁场富集的导磁条对准， 并经过传感器。 传感器和导磁条集成在半导体芯片上。
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