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    Substituted, non-coding nucleic acid molecule for therapeutic and prophylactic stimulation of the immune system in humans and higher animals
    2.
    发明申请
    Substituted, non-coding nucleic acid molecule for therapeutic and prophylactic stimulation of the immune system in humans and higher animals 有权
    用于治疗和预防性刺激人和高等动物免疫系统的取代的非编码核酸分子

    公开(公告)号：US20070049546A1

    公开(公告)日：2007-03-01

    申请号：US11506766

    申请日：2006-08-18

    Applicant: Bernadette Brzezicha ,  Christiane Juhls ,  Florian Sack ,  Manuel Schmidt ,  Burghardt Wittig ,  Matthias Schroff

    Inventor： Bernadette Brzezicha ,  Christiane Juhls ,  Florian Sack ,  Manuel Schmidt ,  Burghardt Wittig ,  Matthias Schroff

    IPC: A61K48/00 ,  C07H21/04

    CPC classification number: C12N15/117 ,  A61K31/713 ,  A61K39/39 ,  A61K2039/53 ,  A61K2039/55561 ,  C12N2310/351 ,  C12N2310/3513 ,  C12N2310/53

    Abstract: A substituted, non-coding nucleic acid molecule for modulating the activity of the human or animal immune system, a method for the production thereof, and a vaccine containing said substituted, non-coding nucleic acid molecule. The abstract of the disclosure is submitted herewith as required by 37 C.F.R. §1.72(b). As stated in 37 C.F.R. §1.72(b): A brief abstract of the technical disclosure in the specification must commence on a separate sheet, preferably following the claims, under the heading “Abstract of the Disclosure.” The purpose of the abstract is to enable the Patent and Trademark Office and the public generally to determine quickly from a cursory inspection the nature and gist of the technical disclosure. The abstract shall not be used for interpreting the scope of the claims. Therefore, any statements made relating to the abstract are not intended to limit the claims in any manner and should not be interpreted as limiting the claims in any manner.

    Abstract translation: 用于调节人或动物免疫系统的活性的取代的非编码核酸分子，其制备方法和含有所述取代的非编码核酸分子的疫苗。 披露摘要根据37 C.F.R.的要求提交。 §1.72（b）。 如37 C.F.R. §1.72（b）：说明书中技术性披露的简要摘要必须在单独的表格上开始，优选按照权利要求，在“披露摘要”标题下。 摘要的目的是使专利商标局和公众一般能够从粗略检查中快速确定技术披露的性质和要点。 摘要不得用于解释权利要求的范围。 因此，与摘要有关的任何陈述不旨在以任何方式限制权利要求，也不应被解释为以任何方式限制权利要求。

    Circular expression construct for gene therapeutic applications
    3.
    发明申请
    Circular expression construct for gene therapeutic applications 审中-公开
    基因治疗应用的循环表达构建体

    公开(公告)号：US20060183703A1

    公开(公告)日：2006-08-17

    申请号：US11299327

    申请日：2005-12-09

    Applicant: Matthias Schroff ,  Colin Smith

    Inventor： Matthias Schroff ,  Colin Smith

    IPC: A61K48/00 ,  C12P19/34

    CPC classification number: A61K48/005 ,  A61K48/00 ,  A61K2039/53 ,  C07K14/005 ,  C12N15/85 ,  C12N15/88 ,  C12N2730/10122 ,  C12N2810/40 ,  C12N2810/50 ,  C12P19/34

    Abstract: Method for producing a circular minimalist expression construct closed in an annular manner, from a double-strand DNA, an expression construct produced according to said method, and the use of the same in gene therapy and vaccination. The abstract of the disclosure is submitted herewith as required by 37 C.F.R. §1.72(b). As stated in 37 C.F.R. §1.72(b): A brief abstract of the technical disclosure in the specification must commence on a separate sheet, preferably following the claims, under the heading “Abstract of the Disclosure.” The purpose of the abstract is to enable the Patent and Trademark Office and the public generally to determine quickly from a cursory inspection the nature and gist of the technical disclosure. The abstract shall not be used for interpreting the scope of the claims. Therefore, any statements made relating to the abstract are not intended to limit the claims in any manner and should not be interpreted as limiting the claims in any manner.

    Abstract translation: 从双链DNA，根据所述方法制备的表达构建体以环状方式产生环状极简表达构建体的方法及其在基因治疗和疫苗接种中的应用。 披露摘要根据37 C.F.R.的要求提交。 §1.72（b）。 如37 C.F.R. §1.72（b）：说明书中技术性披露的简要摘要必须在单独的表格上开始，优选按照权利要求，在“披露摘要”标题下。 摘要的目的是使专利商标局和公众一般能够从粗略检查中快速确定技术披露的性质和要点。 摘要不得用于解释权利要求的范围。 因此，与摘要有关的任何陈述不旨在以任何方式限制权利要求，也不应被解释为以任何方式限制权利要求。

    Method for making linear, covalently closed DNA constructs
    4.
    发明授权
    Method for making linear, covalently closed DNA constructs 有权
    制备线性共价闭合DNA构建体的方法

    公开(公告)号：US06451563B1

    公开(公告)日：2002-09-17

    申请号：US09503799

    申请日：2000-02-14

    Applicant: Burghardt Wittig ,  Claas Junghans ,  Matthias Schroff

    Inventor： Burghardt Wittig ,  Claas Junghans ,  Matthias Schroff

    IPC: C12P1934

    CPC classification number: C12N15/10 ,  C12N15/66

    Abstract: A process to obtain linear double-stranded covalently closed DNA “dumbbell” constructs from plasmids by restriction digest, subsequent ligation with hairpin oligodesoxyribonucleotides, optionally in the presence of restriction enzyme, and a final digestion with endo- and exonucleolytic enzymes that degrade all contaminating polymeric DNA molecules but the desired construct. The invention also provides a process to obtain said dumbbell constructs employing endonuclease class II enzymes. Furthermore, the invention provides a process to obtain linear, covalently closed DNA molecules, such as plasmids, free from contamination by genomic DNA, by submitting the DNA preparation to a facultative endonucleolytic degradation step and an obligatory exonucleolytic degradation step.

    Abstract translation: 通过限制性消化从质粒中获得线性双链共价闭合的DNA“哑铃”构建体的方法，随后与发夹寡脱氧核糖核苷酸连接，任选地在限制性内切酶存在下进行，并且用内切核酸内切酶和最终消化将所有污染聚合物 DNA分子，但是所需的构建体。 本发明还提供了使用内切核酸酶II类酶获得所述哑铃构建体的方法。 此外，本发明提供了通过将DNA制剂提供到兼性内切核酸酶降解步骤和必需的核酸外解离降解步骤，获得线性，共价闭合的DNA分子（例如质粒，不受基因组DNA污染）的方法。

    Method to separate cells that have been modified with superparamagnetic particles by ballistic transfer
    5.
    发明授权
    Method to separate cells that have been modified with superparamagnetic particles by ballistic transfer 失效
    分离通过弹道转移用超顺磁性粒子修饰的细胞的方法

    公开(公告)号：US06348338B1

    公开(公告)日：2002-02-19

    申请号：US08435388

    申请日：1995-05-05

    Applicant: Burghardt Wittig ,  Matthias Schroff

    Inventor： Burghardt Wittig ,  Matthias Schroff

    IPC: C12N1500

    CPC classification number: C12N13/00 ,  C12N15/87 ,  C12N15/89

    Abstract: The ballistic transfer transfection technology employs a cold gas shock wave to accelerate microprojectiles that carry matter into cells by mechanical force. The present invention relates to a method that enables separation of transfected cells by co-adsorbing polynucleic acids and nanometer-sized superparamagnetic particles onto the microprojectiles. The transfected cells are rendered magnetically susceptible and can thus be separated by retaining them in a strong magnetic field. The use of the technology in a clinical context is facilitated.

    Abstract translation: 弹道转移技术采用冷气冲击波加速通过机械力携带物质进入细胞的微粒子。 本发明涉及通过将多核酸和纳米级超顺磁性颗粒共吸附到微粒上来分离转染细胞的方法。 转染的细胞被磁性敏感，因此可以通过将它们保持在强磁场中来分离。 在临床上使用该技术是有利的。

    Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference
    7.
    发明申请
    Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference 有权
    Dna构建体通过Rna干扰特异性抑制基因表达

    公开(公告)号：US20080311630A1

    公开(公告)日：2008-12-18

    申请号：US12095062

    申请日：2006-11-22

    Applicant: Matthias Schroff ,  Detlef Oswald

    Inventor： Matthias Schroff ,  Detlef Oswald

    IPC: C12N15/63 ,  C07H21/00 ,  C12P19/30

    CPC classification number: C12N15/111 ,  C12N15/113 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/52 ,  C12N2310/53 ,  C12N2330/30

    Abstract: The invention relates to expression constructs for targeted inhibition of gene expression and methods for their production and which, after transfection thereof into eukaryotic cells, are suitable for inhibiting in a targeted manner these cells formation of defined proteins by RNA interference, wherein the method is a three step method requiring no PCR steps and is carried out in one reaction vessel in a few hours and are suitable for multiple gene expression inhibition.

    Abstract translation: 本发明涉及用于靶向抑制基因表达的表达构建体及其生产方法，并且在将其转染入真核细胞后，适于以目标方式抑制这些细胞通过RNA干扰形成所定义的蛋白质，其中所述方法为 三步法不需要PCR步骤，并在几个小时内在一个反应​​容器中进行，适用于多种基因表达抑制。

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