公开(公告)号：US5834247A

公开(公告)日：1998-11-10

申请号：US811492

申请日：1997-03-05

申请人： Donald G. Comb ,  Francine B. Perler ,  William E. Jack ,  Ming-Qun Xu ,  Robert A. Hodges ,  Christopher J. Noren ,  Shaorong S. C. Chong ,  Eric Adam ,  Maurice Southworth

发明人： Donald G. Comb ,  Francine B. Perler ,  William E. Jack ,  Ming-Qun Xu ,  Robert A. Hodges ,  Christopher J. Noren ,  Shaorong S. C. Chong ,  Eric Adam ,  Maurice Southworth

IPC分类号： C07K1/107 ,  C07K14/47 ,  C12N9/12 ,  C12N9/14 ,  C12N9/22 ,  C12N9/24 ,  C12N9/38 ,  C12N15/62 ,  C12N15/66 ,  C12N15/67 ,  C12P21/02 ,  C07K19/00 ,  C12P21/00 ,  C12P21/04

CPC分类号： C12Y302/01081 ,  C07K1/1075 ,  C07K14/47 ,  C07K14/4716 ,  C12N15/62 ,  C12N15/66 ,  C12N15/67 ,  C12N9/1252 ,  C12N9/14 ,  C12N9/22 ,  C12N9/2471 ,  C12P21/02 ,  C12Y302/01023 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/92 ,  C07K2319/95

摘要： The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into or adjacent a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity. The CIVPS may be used in a number of applications including purification of the target protein in a one-step protocol.

摘要翻译： 本发明涉及修饰的蛋白质及其生产方法。 修饰的蛋白质包含插入或邻近靶蛋白质的可控插入蛋白质序列（CIVPS），CIVPS能够在预定条件下以顺式或反式切割或切割修饰的蛋白质，即温度升高，暴露于 通过去磷酸化解除氨基酸残基，用化学试剂或去糖基化处理。 如果需要，修饰的蛋白质可以经受这些条件。 CIVPS也可以插入基本上灭活靶蛋白活性的区域。 CIVPS可用于许多应用，包括以一步方案纯化靶蛋白。