公开(公告)号：US5351118A

公开(公告)日：1994-09-27

申请号：US688545

申请日：1991-06-10

申请人： Max Spinell

发明人： Max Spinell

IPC分类号： G01N15/00 ,  G01N1/38 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N21/64 ,  G01N33/49 ,  G01N21/01 ,  G01N21/05

CPC分类号： G01N15/10 ,  G01N15/1404 ,  G01N1/38 ,  G01N2015/1037 ,  G01N2015/1254 ,  G01N2015/1409 ,  G01N2015/1418 ,  G01N21/64 ,  Y10S436/805 ,  Y10S436/806

摘要： An apparatus and a method for analyzing particles suspended in a liquid are described, which particles are of a type or are prepared so as to emit radiation characteristic of the particles when exposed to radiation. Particular fluorescence-photometrical flow cytometry is described in connection with biological cell populations.The apparatus comprises an elongated flow area in the shape of a channel provided in a cuvette comprising a first body member having a planar facial surface and a second body member having a planar facial surface. The first and the second body members are adapted to be moved relative to one another between a first position, in which the planar facial surfaces are in fluid-tight contact with one another, and a second position, in which the planar facial surfaces are spaced apart. At least one of the planar facial surfaces of one of the body members is provided with an elongated groove. In the first position, the first and second body members together define the cuvette in which the elongated flow area is defined by the elongated groove constituting a capillary passage. An inlet for feeding the liquid to the elongated flow area is provided in one of the body members and an outlet for discharging the liquid from the elongated flow area is provided in the very same or in the other body member. At least one of the body members is transparent to light to which the particles are exposed and further transparent to the radiation emitted by the particles. Two electrodes are further described for carrying out an election of particles suspended in the liquid by means of a measurement of the electrical conductivity of the fluid, which particles are of a specific size for subsequent registration of fluorescence when exposed to radiation.

摘要翻译： PCT No.PCT / DK91 / 00120 Sec。 371日期1991年6月10日 102（e）1991年6月10日PCT PCT 1991年5月6日提交PCT公布。 出版物WO91 / 17422 PCT 描述了一种用于分析悬浮在液体中的颗粒的装置和方法，所述装置和方法用于分析悬浮在液体中的颗粒，所述颗粒是暴露于辐射时颗粒的类型或准备以发射颗粒的辐射特征。 结合生物细胞群体描述了特定的荧光光度流式细胞术。 该装置包括设置在比色皿中的通道形状的细长流动区域，其包括具有平坦面部表面的第一主体构件和具有平坦面部表面的第二主体构件。 第一和第二主体构件适于在平面面彼此流体密封的第一位置和第二位置之间相对于彼此移动，在第二位置，平面面部间隔开 分开 身体构件中的一个的平面面中的至少一个设置有细长凹槽。 在第一位置，第一和第二主体部件一起限定了细小流动区域由构成毛细管通道的细长槽限定的比色皿。 用于将液体供给到细长流动区域的入口设置在主体构件中的一个中，并且用于将液体从细长流动区域排出的出口设置在相同或另一个主体构件中。 身体构件中的至少一个对于暴露颗粒的光是透明的，并且对于由颗粒发射的辐射更透明。 进一步描述了两个电极，用于通过流体的电导率的测量来进行悬浮在液体中的颗粒的选择，该颗粒具有特定尺寸，用于当暴露于辐射时随后的荧光配准。

公开(公告)号：US4591445A

公开(公告)日：1986-05-27

申请号：US618517

申请日：1984-06-08

申请人： Max Spinell ,  Bertil Engstrom

发明人： Max Spinell ,  Bertil Engstrom

IPC分类号： G01N33/48 ,  B04B5/00 ,  B04B11/06 ,  C12M1/00 ,  C12M1/26 ,  C12N1/02 ,  C12Q1/24 ,  B01D21/26

CPC分类号： B04B11/06 ,  C12M33/04 ,  C12M33/10 ,  C12M47/02

摘要： In a method of separating bacteria from a bacteria containing liquid sample by means of a dish-like centrifuge container (21) having an upper opening (25) defined by a radially inwardly extending rim portion (24), the following sequential steps are carried out. The centrifuge container is rotated at a high rotational speed. A volume of a high density component of a density higher than the density of the bacteria is introduced into the centrifuge container, whereupon a volume of a low density component having a density lower than the density of the bacteria is introduced into the container so that a two component gradient separation layer (60) comprising a high density layer (61) and a low density layer (62) is formed within the centrifuge container (21). The liquid sample is introduced through a supply tube (32) into the center of the centrifuge container, and owing to the centrifugal force generated by the rotation of the centrifuge container, the liquid is thrown outwardly from the center of the centrifuge container. The bacteria are forced into the low density component layer (62) and deposited in the interface (63) between the two layers (61) and (62), while the liquid sample is discharged (66) from the upper opening of the centrifuge container through a notch (26). A flushing agent is introduced into the centrifuge container and discharged from the upper opening thereof together with any material adhering at the peripheral surface of the gradient separation layer (60). The centrifuge container (21) is decelerated to a low rotational speed, whereafter a suction pipette (40) is activated and moved from a first position having its tip remote from the inner surface of the peripheral wall of the centrifuge container (21) to a second position having its tip arranged adjacent to said inner surface, while a volume of a transfer liquid is introduced into the centrifuge container so that liquid substance included in the centrifuge container is sucked off therefrom through the pipette tube (41) of the suction pipette (40).