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    Systems and methods of polyfocal hyperspectral imaging having a beam splitter with optical channels respectively corresponding to plural image planes
    3.
    发明授权
    Systems and methods of polyfocal hyperspectral imaging having a beam splitter with optical channels respectively corresponding to plural image planes 有权
    具有具有分别对应于多个图像平面的光通道的分束器的多焦点超光谱成像系统和方法

    公开(公告)号：US09429743B2

    公开(公告)日：2016-08-30

    申请号：US14350463

    申请日：2012-10-11

    申请人： Karl Garsha ,  Michael Otter

    发明人： Karl Garsha ,  Michael Otter

    IPC分类号： G02B21/00 ,  G01N21/64 ,  G02B21/36

    CPC分类号： G02B21/367 ,  G01N21/64 ,  G01N21/6458 ,  G02B21/00 ,  G02B21/18 ,  G02B21/361 ,  G02B2207/113

    摘要： A microscope-based system and method for simultaneous imaging of several object planes, of a three-dimensional (3D) sample, associated with different depths throughout the sample. The system includes a polyfocal optical portion, adapted to create a plurality of optical channels each of which is associated with an image of a corresponding object plane, and a spectrally-selective portion, adapted to transform the spectral distribution of the image-forming beam of light to a corresponding spatial distribution. The image, registered by a detector, includes an image of an object plane and an image of the spatially-coded spectral distribution. The method effectuates the simultaneous multispectral imaging of the several object planes. The required data-acquisition time is several fold shorter than that taken by a conventional multispectral microscope-based imaging system.

    摘要翻译： 一种基于显微镜的系统和方法，用于同时成像三维（3D）样品的几个物体平面，与整个样品中的不同深度相关联。 该系统包括多焦点光学部分，其适于创建多个光学通道，每个光学通道与对应的物体平面的图像相关联，以及光谱选择部分，其适于将图像形成光束的光谱分布变换 光照相应的空间分布。 由检测器登记的图像包括物体平面的图像和空间编码的光谱分布的图像。 该方法实现了多个对象平面的同时多光谱成像。 所需的数据采集时间比传统的基于多光谱显微镜的成像系统要短几倍。

    Method for tissue sample fixation
    5.
    发明授权
    Method for tissue sample fixation 有权

    公开(公告)号：US10126216B2

    公开(公告)日：2018-11-13

    申请号：US13372040

    申请日：2012-02-13

    申请人： David Chafin ,  Abbey Pierson Theiss ,  Michael Otter ,  Esteban Roberts

    发明人： David Chafin ,  Abbey Pierson Theiss ,  Michael Otter ,  Esteban Roberts

    IPC分类号： G01N1/30 ,  G01N1/31

    摘要： An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.

    METHOD FOR TISSUE SAMPLE FIXATION
    8.
    发明申请
    METHOD FOR TISSUE SAMPLE FIXATION 审中-公开
    组织样本定位方法

    公开(公告)号：US20120214195A1

    公开(公告)日：2012-08-23

    申请号：US13372040

    申请日：2012-02-13

    申请人： David Chafin ,  Abbey Pierson Theiss ,  Michael Otter ,  Esteban Roberts

    发明人： David Chafin ,  Abbey Pierson Theiss ,  Michael Otter ,  Esteban Roberts

    IPC分类号： G01N1/30

    CPC分类号： G01N1/30 ,  G01N1/31

    摘要： An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.

    摘要翻译： 导致第一温度的醛固定溶液第一次接触组织样品，另外使醛固定液在高于第一温度的第二温度下接触组织样品持续第二时间段。 第一时间段通常为约15分钟至约4小时，第一温度通常为大于0℃至至少15℃。第二温度通常为大于约22℃至 约55℃，第二时间段为约1小时至约4小时。 使用这个过程，改进的组织形态和IHC染色以及翻译后修饰信号的优异保存已经在大约4小时内完成，与室温方案相比，24小时，并且观察到更均匀的形态和抗原保存。

    Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue
    9.
    发明授权
    Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue 有权
    多模态对比和明场上下文渲染，用于增强组织中的病理学确定和多分析物检测

    公开(公告)号：US09310302B2

    公开(公告)日：2016-04-12

    申请号：US13499959

    申请日：2010-10-07

    申请人： Karl Garsha ,  Gary Pestano ,  Michael Otter ,  Alexandra Dea Nagy ,  Ray B. Nagle ,  Phillip Miller ,  Jan Froehlich ,  William Day

    发明人： Karl Garsha ,  Gary Pestano ,  Michael Otter ,  Alexandra Dea Nagy ,  Ray B. Nagle ,  Phillip Miller ,  Jan Froehlich ,  William Day

    IPC分类号： G06K9/36 ,  G01N21/64

    CPC分类号： G01N21/6456

    摘要： Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

    摘要翻译： 可以使用多重模态对比来产生可以组合和渲染的图像，以产生类似于在透射白光照明下观察到的波长吸收染色产生的图像的图像。 使用其他补充对比模式获得的图像可以使用基于用于显示相同解剖结构和组织化学的经典对比度方法的工程配色方案来呈现，从而提供与医学培训和经验相关的图像。 将衍生自折射率和荧光DAPI复染图像的暗视野对比图像组合以产生与用于病理解释的常规H＆E染色获得的图像相似的图像。 这种多模式图像数据可以流式传输用于组织学样本的实时导航，并且可以与遗传DNA探针（FISH），mRNA表达位点（mRNA-ISH）和定位于其上的免疫组织化学（IHC）探针的分子定位相结合 相同的组织切片，用于评估和绘制用于成像质谱法制备的组织切片。

    Adaptive pattern correction for laser scanners
    10.
    发明授权
    Adaptive pattern correction for laser scanners 有权
    激光扫描仪的自适应图案校正

    公开(公告)号：US08057463B2

    公开(公告)日：2011-11-15

    申请号：US11400552

    申请日：2006-04-07

    申请人： Ruben Zadoyan ,  Michael Karavitis ,  Peter Goldstein ,  Mike White ,  Michael Otter

    发明人： Ruben Zadoyan ,  Michael Karavitis ,  Peter Goldstein ,  Mike White ,  Michael Otter

    IPC分类号： A61B18/20

    CPC分类号： G02B26/101 ,  A61F9/00825 ,  A61F2009/00872 ,  A61F2009/00897

    摘要： A system for adaptive laser scanning correction includes a laser scanner coupled to a controller. The controller develops control signals for the laser scanner for a directed scan pattern that is modified to compensate for a characteristic scan-pattern distortion introduced by the laser scanner. The laser scanner responds to the control signals to provide an actual scan pattern approaching a target scan-pattern shape. The system may be useful for ophthalmologic laser surgery and other applications requiring precise control over scan pattern shape and a high scanning speed.

    摘要翻译： 用于自适应激光扫描校正的系统包括耦合到控制器的激光扫描仪。 控制器为激光扫描器开发用于定向扫描图案的控制信号，其被修改以补偿由激光扫描器引入的特征扫描图案失真。 激光扫描器响应于控制信号以提供接近目标扫描图案形状的实际扫描图案。 该系统可用于需要精确控制扫描图案形状和高扫描速度的眼科激光手术和其它应用。