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1.发明申请DEVICE FOR RAPID IDENTIFICATION OF NUCLEIC ACIDS FOR BINDING TO SPECIFIC CHEMICAL TARGETS 审中-公开用于快速鉴定用于结合特定化学目标的核酸的装置公开(公告)号：US20120028811A1
公开(公告)日：2012-02-02
申请号：US13059223
申请日：2009-08-17
申请人： Harold G. Craighead , John T. Lis , Seungmin Park , So Youn Kim , Jiyoung Ahn , Minjoung Jo
发明人： Harold G. Craighead , John T. Lis , Seungmin Park , So Youn Kim , Jiyoung Ahn , Minjoung Jo
CPC分类号： C12Q1/6811 , B01L3/502707 , B01L3/502715 , B01L3/502753 , B01L7/52 , B01L2200/10 , B01L2300/069 , B01L2300/0816 , B01L2300/0861 , B01L2300/0883 , B01L2300/1827 , B01L2400/0487 , C12Q1/686 , C12Q2525/205 , Y10T156/10 , C12Q2565/629 , C12Q2541/101
摘要： The present invention relates to microfluidic chips and their use in SELEX. The microfluidic chip preferably includes a reaction chamber that contains a high surface area material that contains target. One preferred high surface area material is a sol-gel derived material. Methods of making the microfluidic chips are described herein, as are uses of these devices to select aptamers against the target.
摘要翻译： 本发明涉及微流控芯片及其在SELEX中的应用。 微流体芯片优选包括含有包含靶的高表面积材料的反应室。 一种优选的高表面积材料是溶胶 - 凝胶衍生材料。 这里描述了制造微流体芯片的方法，以及这些装置用于选择针对靶的适体的方法。
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公开(公告)号：US10221412B2
公开(公告)日：2019-03-05
申请号：US13877862
申请日：2011-10-05
申请人： So Youn Kim , Ji-Young Ahn , Minjoung Jo , Tae Kyung Kim
发明人： So Youn Kim , Ji-Young Ahn , Minjoung Jo , Tae Kyung Kim
IPC分类号： C12N15/10 , C40B30/04 , C12N15/11 , C12N15/115 , G01N33/53
摘要： The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques. Particularly, when a process for selecting aptamers is performed using the device of the invention together with a high-throughput sequencing method, the number of target binding/elution/amplification rounds can be greatly reduced, and the process can be performed in an automated manner. Thus, the device of the invention is highly useful.
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3.发明授权Sol-gel kit for preparing biochip and method for preparing biochip using the same 有权用于制备生物芯片的溶胶凝胶试剂盒及其制备方法公开(公告)号：US09101902B2
公开(公告)日：2015-08-11
申请号：US13096991
申请日：2011-04-28
申请人： Minjoung Jo , Seram Lee
发明人： Minjoung Jo , Seram Lee
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/0036 , B01J2219/00364 , B01J2219/00385 , B01J2219/00599 , B01J2219/00637 , B01J2219/00644 , B01J2219/00657 , B01J2219/00725
摘要： A method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB1, SolB2 and SolB3, SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing.
摘要翻译： 一种通过凝胶化溶胶组合物制备蛋白质芯片的方法。 在该方法中，依次混合特定硅酸盐单体如SolB1，SolB2和SolB3，SolBH和SolBS，蒸馏水和检测器蛋白的混合物的混合物，使得溶胶组合物的凝胶化速率可以延迟， 从而导致组合物的稳定凝胶化。 此外，生物芯片可以通过使用排液器或诸如移液管的工具通过手动分配溶胶组合物以简单和容易的方式制造。 此外，可以通过将溶胶组合物溶液I（SolBH）和溶液II（缓冲液，SolBS，蒸馏水和检测器蛋白质的混合物）顺序分配到基底上而不需要常规的预处理过程例如 混合。
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4.发明申请MULTIPLEX MICROFLUIDIC DEVICE FOR SELECTING NUCLEIC ACID APTAMERS, AND HIGH THROUGHPUT SELECTION METHOD FOR NUCLEIC ACID APTAMERS USING SAME 审中-公开用于选择核酸APTAMERS的多重微流体装置，以及使用相同的核酸APTAMERS的高通量选择方法公开(公告)号：US20130274113A1
公开(公告)日：2013-10-17
申请号：US13877862
申请日：2011-10-05
申请人： So Youn Kim , Ji-Young Ahn , Minjoung Jo , Tae Kyung Kim
发明人： So Youn Kim , Ji-Young Ahn , Minjoung Jo , Tae Kyung Kim
IPC分类号： C12N15/10
CPC分类号： C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/11 , C12N2320/13 , G01N33/5308 , G01N2500/00
摘要： The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques. Particularly, when a process for selecting aptamers is performed using the device of the invention together with a high-throughput sequencing method, the number of target binding/elution/amplification rounds can be greatly reduced, and the process can be performed in an automated manner. Thus, the device of the invention is highly useful.
摘要翻译： 本发明涉及用于选择核酸适体的多重微流体装置和使用其的核酸适配体的高通量选择方法，更具体地说，涉及一种复合微流体装置（SELEX lap-on-a-chip），其中 使用改进的多路复用平台代替单个靶的适配体的开发，以及使用其的高通量测序的适配体的高通量选择的方法。 根据本发明的多重微流体装置可以同时检测多个靶的适体，并且与传统的多重技术相比，其可大大增加筛选通过量并大大缩短处理时间。 特别地，当使用本发明的装置和高通量测序方法进行选择适体的方法时，可以大大减少目标结合/洗脱/放大倍数的数量，并且可以以自动方式进行处理 。 因此，本发明的装置是非常有用的。
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5.发明申请SOL-GEL KIT FOR PREPARING BIOCHIP AND METHOD FOR PREPARING BIOCHIP USING THE SAME 有权用于制备生物化学品的溶胶凝胶试剂盒及其制备方法公开(公告)号：US20120277108A1
公开(公告)日：2012-11-01
申请号：US13096991
申请日：2011-04-28
申请人： Minjoung JO , Seram Lee
发明人： Minjoung JO , Seram Lee
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/0036 , B01J2219/00364 , B01J2219/00385 , B01J2219/00599 , B01J2219/00637 , B01J2219/00644 , B01J2219/00657 , B01J2219/00725
摘要： A method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB1, SolB2 and SolB3, SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing.
摘要翻译： 一种通过凝胶化溶胶组合物制备蛋白质芯片的方法。 在该方法中，依次混合特定硅酸盐单体如SolB1，SolB2和SolB3，SolBH和SolBS，蒸馏水和检测器蛋白的混合物的混合物，使得溶胶组合物的凝胶化速率可以延迟， 从而导致组合物的稳定凝胶化。 此外，生物芯片可以通过使用排液器或诸如移液管的工具通过手动分配溶胶组合物以简单和容易的方式制造。 此外，可以通过将溶胶组合物溶液I（SolBH）和溶液II（缓冲液，SolBS，蒸馏水和检测器蛋白质的混合物）顺序分配到基底上而不需要常规的预处理过程例如 混合。
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