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    DAB-CONTAINING SUBSTRATE KIT FOR DYEING USE WHICH IS PRODUCED USING LABELLING ENZYME
    2.
    发明申请
    DAB-CONTAINING SUBSTRATE KIT FOR DYEING USE WHICH IS PRODUCED USING LABELLING ENZYME 审中-公开
    含有DAB的基材包用于使用标签酶的生产使用

    公开(公告)号：US20160010140A1

    公开(公告)日：2016-01-14

    申请号：US14770985

    申请日：2014-02-28

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Toshiyuki KASAMATSU ,  Yuriko KITANO

    IPC分类号： C12Q1/28 ,  G01N33/53

    CPC分类号： C12Q1/28 ,  G01N33/5306

    摘要： Provided is a DAB-containing substrate kit that is capable of enhancing specific staining intensity and reducing precipitation due to DAB aggregation in DAB-containing substrate solution and nonspecific staining, such as background staining, in chromogenic reaction using a peroxidase-labeled antibody, by the use of chromogenic reaction solution containing DAB and its sensitizer imidazole. The kit includes chromophore-containing solution (A) containing a peroxidase chromophore DAB and water; and chromogenic reagent (B) containing: at least one chelating agent selected from the group consisting of EDTA-2Na, EDTA-2NH4, GEDTA, and NTA; POE and/or POP alkyl ether nonionic surfactant; imidazole; hydrogen peroxide; and water. The kit is in a system of two- or more components with at least the chromophore-containing solution (A) and the chromogenic reagent (B) being separately stored.

    摘要翻译： 本发明提供了一种含DAB的底物试剂盒，其能够增强特异性染色强度并减少由于DAB在含有DAB的底物溶液中的聚集引起的沉淀和非特异性染色，例如背景染色，使用过氧化物酶标记的抗体在显色反应中 使用含有DAB及其敏化剂咪唑的显色反应溶液。 该试剂盒包含含有过氧化物酶发色团DAB和水的含发色团溶液（A） 和显色试剂（B），其含有：至少一种选自EDTA-2Na，EDTA-2NH4，GEDTA和NTA的螯合剂; POE和/或POP烷基醚非离子表面活性剂; 咪唑 过氧化氢; 和水。 该试剂盒在两个或多个组分的系统中，至少含有发色团的溶液（A）和显色剂（B）被分开储存。

    Combination product for detecting target marker
    3.
    发明授权
    Combination product for detecting target marker 有权

    公开(公告)号：US10324084B2

    公开(公告)日：2019-06-18

    申请号：US15305461

    申请日：2015-04-23

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Hirokazu Ohbayashi ,  Kayoko Fujita

    IPC分类号： G01N33/53 ,  G01N33/536 ,  C12Q1/68 ,  G01N33/532 ,  G01N33/535 ,  G01N33/58 ,  C12Q1/28 ,  C12Q1/42

    摘要： The present invention relates to a combination product for detecting a target marker simply and with high sensitivity. More specifically, the present invention relates to a combination product for detecting a target marker in a biological sample in combination with a target marker binding molecule which is capable of binding specifically to the target marker in the biological sample, the combination comprising, at least: (a) a first binding agent comprising a first binding molecule which is capable of directly or indirectly binding specifically to the target marker binding molecule, and a labeling substance; (b) a linker molecule which is capable of binding specifically to the first binding agent; and (c) a second binding agent which is capable of binding specifically to the linker molecule, and comprises a second binding molecule and a labeling substance.

    Pretreatment solution for immunohistochemical staining and condensed solution thereof
    5.
    发明授权
    Pretreatment solution for immunohistochemical staining and condensed solution thereof 有权

    公开(公告)号：US09645056B2

    公开(公告)日：2017-05-09

    申请号：US14299166

    申请日：2014-06-09

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Toshiyuki Kasamatsu

    IPC分类号： G01N1/30 ,  G01N33/53

    CPC分类号： G01N1/30 ,  G01N33/5306

    摘要： Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution and a method of immunohistochemical staining using the same. The pretreatment solution contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.

    COMBINATION PRODUCT FOR DETECTING TARGET MARKER
    6.
    发明申请
    COMBINATION PRODUCT FOR DETECTING TARGET MARKER 审中-公开
    用于检测目标标记的组合产品

    公开(公告)号：US20170045502A1

    公开(公告)日：2017-02-16

    申请号：US15305461

    申请日：2015-04-23

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Hirokazu OHBAYASHI ,  Kayoko FUJITA

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5306 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/532 ,  G01N33/535 ,  G01N33/536 ,  G01N33/58 ,  G01N33/581

    摘要： The present invention relates to a combination product for detecting a target marker simply and with high sensitivity. More specifically, the present invention relates to a combination product for detecting a target marker in a biological sample in combination with a target marker binding molecule which is capable of binding specifically to the target marker in the biological sample, the combination comprising, at least: (a) a first binding agent comprising a first binding molecule which is capable of directly or indirectly binding specifically to the target marker binding molecule, and a labeling substance; (b) a linker molecule which is capable of binding specifically to the first binding agent; and (c) a second binding agent which is capable of binding specifically to the linker molecule, and comprises a second binding molecule and a labeling substance.

    摘要翻译： 本发明涉及用于简单且高灵敏度地检测目标标记物的组合产品。 更具体地，本发明涉及一种用于检测生物样品中靶标记物与能够特异性结合生物样品中靶标记物的靶标记结合分子组合的组合产物，该组合包括至少： （a）第一结合剂，其包含能够直接或间接结合靶标标结合分子的第一结合分子和标记物质; （b）能够特异性结合第一结合剂的连接分子; 和（c）能够特异性结合连接分子的第二结合剂，并且包含第二结合分子和标记物质。

    PRETREATMENT SOLUTION FOR IMMUNOHISTOCHEMICAL STAINING AND CONDENSED SOLUTION THEREOF
    7.
    发明申请
    PRETREATMENT SOLUTION FOR IMMUNOHISTOCHEMICAL STAINING AND CONDENSED SOLUTION THEREOF 审中-公开
    免疫组织化学沉淀及浓缩溶液的预处理方法

    公开(公告)号：US20140287429A1

    公开(公告)日：2014-09-25

    申请号：US14299166

    申请日：2014-06-09

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Toshiyuki KASAMATSU

    IPC分类号： G01N1/30

    CPC分类号： G01N1/30 ,  G01N33/5306

    摘要： Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution and a method of immunohistochemical staining using the same. The pretreatment solution contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.

    摘要翻译： 公开了一种用于免疫组织化学染色的预处理溶液，其从包含培养基的组织标本的载玻片洗脱含石蜡包埋培养基，并检索组织标本的抗原性，并且其可用于三次或更多次，以及预处理 溶液浓缩物用于免疫组织化学染色，其允许即时制备预处理溶液和使用其进行免疫组织化学染色的方法。 预处理溶液含有抗原检索剂，特定的非离子表面活性剂和环糊精或其衍生物，余量不小于80质量％的水。 抗原提取剂的含量使得预处理溶液的pH在预定范围内，并且环糊精或其衍生物的含量是特定的量。

    Combination product for detecting target marker
    8.
    发明授权
    Combination product for detecting target marker 有权

    公开(公告)号：US11156602B2

    公开(公告)日：2021-10-26

    申请号：US16399050

    申请日：2019-04-30

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Hirokazu Ohbayashi ,  Kayoko Fujita

    IPC分类号： G01N33/58 ,  G01N33/53 ,  C12Q1/68 ,  G01N33/532 ,  G01N33/535 ,  G01N33/536 ,  C12Q1/28 ,  C12Q1/42

    摘要： The present invention relates to a combination product for detecting a target marker simply and with high sensitivity. More specifically, the present invention relates to a combination product for detecting a target marker in a biological sample in combination with a target marker binding molecule which is capable of binding specifically to the target marker in the biological sample, the combination comprising, at least: (a) a first binding agent comprising a first binding molecule which is capable of directly or indirectly binding specifically to the target marker binding molecule, and a labeling substance; (b) a linker molecule which is capable of binding specifically to the first binding agent; and (c) a second binding agent which is capable of binding specifically to the linker molecule, and comprises a second binding molecule and a labeling substance.

    Automatic tissue staining device and automatic tissue staining method
    9.
    发明授权
    Automatic tissue staining device and automatic tissue staining method 有权

    公开(公告)号：US10684200B2

    公开(公告)日：2020-06-16

    申请号：US16139569

    申请日：2018-09-24

    申请人： NICHIREI BIOSCIENCES INC.

    发明人： Toshiyuki Kasamatsu ,  Noboru Horikoshi

    IPC分类号： G01N1/00 ,  G01N1/31 ,  G01N1/28 ,  G01N1/30 ,  G01N33/48 ,  G01N35/00

    摘要： An automatic tissue staining device includes a supply head that supplies a treatment fluid, a horizontal direction movement unit that moves the supply head in a horizontal direction, and a holding unit that holds a plurality of glass slides on which samples are set. The automatic tissue staining device further includes a control unit that judges an occupancy status of the horizontal direction movement unit in a condition that one or more of the glass slides are situated in a first region prior to supplying the treatment fluid from the supply head to one or more samples in the first region, suspends a start of a treatment for the samples in the first region when the horizontal direction movement unit is occupied, and permits the treatment for the samples in the first region when the horizontal direction movement unit is not occupied.

    TISSUE SLICE SELECTION METHOD, AND EMBEDDED BLOCK PREPARING CASSETTE
    10.
    发明申请
    TISSUE SLICE SELECTION METHOD, AND EMBEDDED BLOCK PREPARING CASSETTE 审中-公开

    公开(公告)号：US20200149999A1

    公开(公告)日：2020-05-14

    申请号：US16609074

    申请日：2018-04-27

    申请人： NIHON UNIVERSITY ,  NICHIREI BIOSCIENCES INC.

    发明人： Shinobu MASUDA ,  Ryohei SUZUKI ,  Yuriko KITANO

    IPC分类号： G01N1/06 ,  G01N1/30 ,  G01N1/36 ,  G01N33/52

    摘要： The present invention is to provide a tissue slice selection method and an embedded block-preparing cassette which are capable of suppressing occurrence of errors in inspection results. The present invention makes it possible to suppress the occurrence of errors in inspection results by including: an embedded block-preparing process of preparing an embedded block by embedding both a biological tissue fragment and a standard substance in the same embedding agent; an embedded tissue slice preparing step of slicing the embedded block to prepare a sheet-like embedded tissue slice having a tissue slice and standard substance slices appearing on a surface thereof; and a tissue slice selecting step of selecting the tissue slice to be inspected, based on a light signal from the standard substance slice.