公开(公告)号：US09175234B2

公开(公告)日：2015-11-03

申请号：US12645497

申请日：2009-12-23

申请人： Louis Hom ,  Na Trinh ,  Murtaza Alibhai ,  Zhihao Hu ,  Eli Groban ,  Vikranth Arlagadda ,  Elizabeth Clarke

发明人： Louis Hom ,  Na Trinh ,  Murtaza Alibhai ,  Zhihao Hu ,  Eli Groban ,  Vikranth Arlagadda ,  Elizabeth Clarke

IPC分类号： C12N9/18 ,  C12N15/00 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04 ,  C10L1/02 ,  C12N9/16

CPC分类号： C12N9/18 ,  C07H21/04 ,  C10L1/02 ,  C12N9/16 ,  C12N15/00 ,  C12N15/63 ,  C12P21/06

摘要： The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

摘要翻译： 本发明涉及新的突变硫酯酶及其天然存在的等同物，由这些酶制成的组合物和硫酯酶的用途。 特别地，本发明提供突变体硫酯酶，其具有改变的性质，例如改变的底物特异性，改变的活性，改变的选择性和/或在产物混合物中改变的比例产率。 本发明还提供了编码这种突变体硫酯酶的多核苷酸，以及包含这种多核苷酸的载体和宿主细胞。 本发明进一步提供硫酯酶在生产各种脂肪酸衍生物中的新用途，其可用作或作为工业化学品和燃料的组分。

公开(公告)号：US20150361454A1

公开(公告)日：2015-12-17

申请号：US14761299

申请日：2014-01-16

申请人： Mathew RUDE ,  Na TRINH ,  Andreas SCHIRMER ,  Jacob GANO ,  REG LIFE SCIENCES, LLC

发明人： Mathew RUDE ,  Na TRINH ,  Andreas SCHIRMER ,  Jacob GANO

IPC分类号： C12P7/04 ,  C12N9/02

CPC分类号： C12N9/0008 ,  C12P7/04 ,  C12P7/24 ,  C12P7/6409 ,  C12Y102/0108

摘要： The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

摘要翻译： 本公开涉及当在重组宿主细胞中表达时导致脂肪醛和脂肪醇产生改善的酰基-ACP还原酶（AAR）酶变体。 本公开还涉及制备和使用这种AAR变体用于生产具有特定特征的脂肪醇组合物的方法。

公开(公告)号：US20100154293A1

公开(公告)日：2010-06-24

申请号：US12645497

申请日：2009-12-23

申请人： Louis Hom ,  Na Trinh ,  Murtaza Alibhai

发明人： Louis Hom ,  Na Trinh ,  Murtaza Alibhai

IPC分类号： C10L1/188 ,  C12N9/16 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P7/64 ,  C08G63/48

CPC分类号： C12N9/18 ,  C07H21/04 ,  C10L1/02 ,  C12N9/16 ,  C12N15/00 ,  C12N15/63 ,  C12P21/06

摘要： The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

摘要翻译： 本发明涉及新的突变硫酯酶及其天然存在的等同物，由这些酶制成的组合物和硫酯酶的用途。 特别地，本发明提供突变体硫酯酶，其具有改变的性质，例如改变的底物特异性，改变的活性，改变的选择性和/或在产物混合物中改变的比例产率。 本发明还提供了编码这种突变体硫酯酶的多核苷酸，以及包含这种多核苷酸的载体和宿主细胞。 本发明进一步提供硫酯酶在生产各种脂肪酸衍生物中的新用途，其可用作或作为工业化学品和燃料的组分。