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    Enhanced probe binding
    1.
    发明授权
    Enhanced probe binding 有权

    公开(公告)号：US10294516B2

    公开(公告)日：2019-05-21

    申请号：US14157136

    申请日：2014-01-16

    申请人： Nabsys 2.0 LLC

    发明人： John S. Oliver

    IPC分类号： C12P19/34 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6858

    摘要： Methods for enhancing the binding of oligonucleotide probes to DNA and RNA are disclosed. The methods make use of thermodynamic and kinetic effects to reduce probe mismatches and failure of complementary probes to bind to DNA and RNA templates. Mapping and sequencing of the probed DNA and RNA samples are contemplated herein.

    DISTANCE MAPS USING MULTIPLE ALIGNMENT CONSENSUS CONSTRUCTION
    2.
    发明申请
    DISTANCE MAPS USING MULTIPLE ALIGNMENT CONSENSUS CONSTRUCTION 审中-公开
    使用多种对齐方式建立的距离

    公开(公告)号：US20170024514A1

    公开(公告)日：2017-01-26

    申请号：US15287504

    申请日：2016-10-06

    申请人： Nabsys 2.0 LLC

    发明人： Peter Goldstein ,  William Heaton ,  Franco Preparata ,  Eli Upfal

    IPC分类号： G06F19/22

    CPC分类号： G16B30/00

    摘要： Techniques for assembly of genetic maps including de novo assembly of distance maps using multiple alignment consensus construction. Multiple map alignment can be performed on a defined bundle of fragment maps corresponding to biomolecule fragments to determine consensus events and corresponding locations. Fragment maps in the bundle can be removed when there is no overhang from the consensus events. When the subset of fragment maps in the bundle is less than a predetermined threshold, one or more additional fragment maps can be added based on fragment signatures, a consensus alignment score, and a pairwise alignment score. Techniques for multiple alignment can include generating a graph with edges and vertices representing each pairwise relation. An ordered set of sets of events best representing a multiple alignment reflecting all pairwise alignments can be generated by repeatedly randomly removing edges and combining vertices to identify a min cut of the graph.

    摘要翻译： 用于组合遗传图谱的技术，包括使用多重对准共识构造的距离图的从头装配。 可以在对应于生物分子片段的确定的片段图上进行多个图谱比对，以确定共有事件和相应的位置。 当共识事件没有悬而未决时，可以删除该分组中的片段映射。 当束中的片段映射子集小于预定阈值时，可以基于片段签名，共有对齐分数和成对比对分数来添加一个或多个附加片段图。 用于多对齐的技术可以包括生成具有表示每个成对关系的边和顶点的图。 可以通过重复随机地去除边和组合顶点以识别图的最小切割来生成最佳表示反映所有成对比对的多重对齐的事件组的有序集合。

    Assay methods using nicking endonucleases
    3.
    发明授权
    Assay methods using nicking endonucleases 有权
    使用切口内切核酸酶的测定方法

    公开(公告)号：US09434981B2

    公开(公告)日：2016-09-06

    申请号：US14199434

    申请日：2014-03-06

    申请人： Nabsys 2.0 LLC

    发明人： John S. Oliver

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q2521/307 ,  C12Q2522/101 ,  C12Q2531/119 ,  C12Q2565/629 ,  C12Q2565/631 ,  C12Q2521/301

    摘要： Assay methods and apparatus for the analysis of biopolymers are disclosed. The assays employ nicking endonucleases to enable the generation of flaps on target biomolecules which are detected in nanopore or fluidic channel devices. Identification of flap locations enables a map of the target biomolecule to be derived.

    摘要翻译： 公开了用于分析生物聚合物的测定方法和设备。 该测定法使用切口内切核酸酶以使得能够在纳米孔或流体通道器件中检测到的靶生物分子上产生襟翼。 识别皮瓣位置使得能够导出目标生物分子的图谱。

    Self-sealing pipette septum
    4.
    发明授权
    Self-sealing pipette septum 有权

    公开(公告)号：US10583441B2

    公开(公告)日：2020-03-10

    申请号：US14852086

    申请日：2015-09-11

    申请人： Nabsys 2.0 LLC

    发明人： Jeffrey H. Stokes ,  Ryan C. Almeida

    IPC分类号： B01L3/00 ,  A61J1/14 ,  A61M39/04 ,  G01N1/28 ,  A61M39/00

    摘要： A liquid injection port including a liquid input block defining a liquid input conduit; a compression block adapted to mate with the liquid input block; and a septum including a deformable material mounted in a septum retainer, the septum defining a central perforation, and forming a seal between the liquid input block and the compression block. The septum defines a conical deformation toward the liquid input conduit. A method for delivering fluid from a pipette tip includes a) introducing the fluid into the pipette tip; b) inserting the pipette tip into a pipette conduit defined by a compression block; c) inserting the pipette tip through a septum mounted in the compression block; and d) releasing the fluid in the pipette tip into a liquid input conduit defined in a liquid input block, the septum defining a conical deformation toward the liquid input conduit while maintaining a seal.

    Devices and methods for determining the length of biopolymers and distances between probes bound thereto
    6.
    发明授权
    Devices and methods for determining the length of biopolymers and distances between probes bound thereto 有权

    公开(公告)号：US09719980B2

    公开(公告)日：2017-08-01

    申请号：US14331629

    申请日：2014-07-15

    申请人： Nabsys 2.0 LLC

    发明人： John S. Oliver

    IPC分类号： G01N33/487 ,  G01N27/327 ,  G01N27/447

    CPC分类号： G01N33/48721 ,  G01N27/3278 ,  G01N27/4473 ,  Y10S977/957

    摘要： Devices and methods for detecting the length of analytes and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained (e.g., as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals.